2. MCE Kits
  3. Protein Biology
  4. Protein Electrophoresis & WB
  5. Blocking Buffer
  6. Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×)

Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×) 

Cat. No.: HY-K1027
Manual SDS

MCE Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×) mainly consists of fish gelatin protein, and is widely used for the antibody blocking step in Western Blot or ELISA within 15 minutes.

Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×)
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10 pouches USD 50 In-stock
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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

MCE Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×) mainly consists of fish gelatin protein. Each pouch can be diluted to 100 mL 1× blocking buffer, in which the concentration of the protein is 5%. Containing Tween 20, this product is widely used for the antibody blocking step in Western Blot or ELISA within 15 minutes.

This product provides a convenient way to make blocking buffer and eliminates the need to weigh and mix individual components.

Storage

Store at room temperature for 3 years.

Protocol

1.   Prepare the working buffer

a.   Add 1 pouch of Rapid Blocking Buffer (TBS-T) Powder into the cleaned beaker, dissolve with 50 mL distilled water under a magnetic stirrer.

b.   Add distilled water to the solution in step 1 until the total volume is 100 mL. The final solution is 1× working buffer.

2.    Blocking step

a.   Place the membrane into the incubator box, add 10-20 mL 1× working buffer and incubate in the shaker incubator at room temperature for about 10 minutes.

b.   The blocked membrane can be used for the subsequent experiments.

Components
Components HY-K1027-10 pouches
Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×) 10 pouches
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×)
Cat. No.:
HY-K1027
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