Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×) 

MCE Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×) mainly consists of fish gelatin protein. Each pouch can be diluted to 100 mL 1× blocking buffer, in which the concentration of the protein is 5%. Containing Tween 20, this product is widely used for the antibody blocking step in Western Blot or ELISA within 15 minutes.

This product provides a convenient way to make blocking buffer and eliminates the need to weigh and mix individual components.

Store at room temperature for 3 years.

1. Prepare the working buffer

a. Add 1 pouch of Rapid Blocking Buffer (TBS-T) Powder into the cleaned beaker, dissolve with 50 mL distilled water under a magnetic stirrer.

b. Add distilled water to the solution in step 1 until the total volume is 100 mL. The final solution is 1× working buffer.

2. Blocking step

a. Place the membrane into the incubator box, add 10-20 mL 1× working buffer and incubate in the shaker incubator at room temperature for about 10 minutes.

b. The blocked membrane can be used for the subsequent experiments.