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Carbonic Anhydrase 2 Antibody (YA3362)(PBS only)

(Synonyms: CA2; Carbonic anhydrase 2; Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II)

Carbonic Anhydrase 2 Antibody (YA3362) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Carbonic Anhydrase 2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Carbonic Anhydrase 2 Antibody (YA3362)(PBS only)
  • Carbonic Anhydrase 2 Antibody (YA3362)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Carbonic Anhydrase 2 Antibody (YA3362) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Carbonic Anhydrase 2.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 29 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 29 kDa
Immunogen

A synthesized peptide derived from human Carbonic anhydrase 2

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Carbonic Anhydrase 2 Antibody (YA3362)(PBS only)
    Western blot analysis was performed on extracts from A431 (lane 1, 20 μg), Mouse kidney (lane 2, 20 μg), and Rat kidney (lane 3, 20 μg) using Carbonic Anhydrase 2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Carbonic Anhydrase 2 catalyzes the reversible hydration of carbon dioxide. Can also hydrate cyanamide to urea. Stimulates the chloride-bicarbonate exchange activity of SLC26A6. Essential for bone resorption and osteoclast differentiation. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53].

  • Subcellular Localization

    Cytoplasm; Cell membrane

  • Subunit

    Interacts with SLC4A4 (PubMed:14567693, PubMed:15218065). Interaction with SLC4A7 regulates SLC4A7 transporter activity (PubMed:14736710). Interacts with SLC26A6 isoform 4 (via C-terminus cytoplasmic domain) (PubMed:15990874)

  • SwissProt ID

    P00918

  • Gene ID
    760 [NCBI]
  • Synonyms

    CA2; Carbonic anhydrase 2; Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II

  • Research Field

    Cardiovascular

Carbonic Anhydrase 2 Antibody (YA3362)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from A431 (lane 1, 20 μg), Mouse kidney (lane 2, 20 μg), and Rat kidney (lane 3, 20 μg) using Carbonic Anhydrase 2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Carbonic Anhydrase 2 Antibody (YA3362)(PBS only) Product Image
    1/16
  • Experimental Validation Results for Carbonic Anhydrase 2 Antibody (YA3362)(PBS only)
  • Carbonic Anhydrase 2 Antibody (YA3362)(PBS only) Product Image
  • Experimental Validation Results for Carbonic Anhydrase 2 Antibody (YA3362)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from A431 (lane 1, 20 μg), Mouse kidney (lane 2, 20 μg), and Rat kidney (lane 3, 20 μg) using Carbonic Anhydrase 2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Carbonic Anhydrase 2 Antibody (YA3362)(PBS only)
  • Carbonic Anhydrase 2 Antibody (YA3362)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from A431 (lane 1, 20 μg), Mouse kidney (lane 2, 20 μg), and Rat kidney (lane 3, 20 μg) using Carbonic Anhydrase 2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit/Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Carbonic Anhydrase 2 Antibody (YA3362)(PBS only) Product Image WB