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PEI Transfection Reagent

65 Cited Publications
Customer Review

Based on 65 publication(s) in Google Scholar

MCE PEI Transfection Reagent is designed based on 25 kDa PEI. It has high-efficiency, low-toxicity, strong-stability, and is suitable for many cell types, such as HEK-293、HEK-293T、CHO-K1、COS-1、COS-7、NIH/3T3、Sf9、HepG2 and HeLa et, even some hard-to-transfect cells. It can also be applied to large-scale recombinant protein expression and virus production. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

  • Storage :

    -20°C, 1 year.

    Avoid repetitive freeze-thaw cycles.

  • Experimental Validation Results for PEI Transfection Reagent
  • Experimental Validation Results for PEI Transfection Reagent
  • Experimental Validation Results for PEI Transfection Reagent
  • Experimental Validation Results for PEI Transfection Reagent
  • PEI Transfection Reagent Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Description & Advantages

PEI is a commonly used polymer carrier in gene transfection. It consists of cationic polymers and can introduce nucleic acids into eukaryotic cells. In polymer-based transfection, exogenous DNA forms complexes with cationic polymers that enter host cells by endocytosis.

MCE PEI Transfection Reagent is designed based on 25 kDa PEI. It is modified by introducing functional genes to enhance the binding ability of DNA, and reduced the cytotoxicity. In addition, the cell uptake and the endosome escape functions of PEI modified was enhanced significantly.

MCE PEI Transfection Reagent is high-efficiency, low-toxicity, strong-stability, and suitable for many cell types, such as HEK-293、HEK-293T、CHO-K1、COS-1、COS-7、NIH/3T3、Sf9、HepG2 and HeLa et, even some hard-to-transfect cells. It can also be applied to large-scale recombinant protein expression and virus production.

Protocol

1. Cell Preparation

Seed cells one day before transfection so that they are in good growth condition at the time of transfection. Optimize cell density according to the cell line.

Parameter Recommended starting condition
Cell density Approximately 50%-80% confluence at transfection
Plasmid DNA 1 μg
PEI Transfection Reagent 2.5 μL

2. Preparation of DNA/PEI Complexes

1. Dilute plasmid DNA and PEI Transfection Reagent separately in serum-free medium or another suitable serum-free diluent.

2. Mix gently and incubate each solution for approximately 5 min at room temperature.

3. Add the diluted PEI Transfection Reagent to the diluted DNA, mix gently, and incubate for approximately 15 min at room temperature to form transfection complexes.

3. Transfection

1. Add the DNA/PEI complexes dropwise to the cell culture medium and gently rock the vessel to distribute the complexes evenly.

2. Continue culture under normal cell-culture conditions.

3. Depending on cell type and experimental purpose, replace with fresh complete medium approximately 4-6 h after transfection, or leave the medium unchanged if the cells tolerate the transfection well.

4. Analyze target-gene expression approximately 24-48 h after transfection. Longer culture may be used for recombinant protein production or virus packaging according to the experimental design.

Note: In MCE validation, 1 μg GFP plasmid with 2.5 μL PEI Transfection Reagent showed good transfection performance. Optimize the DNA-to-reagent ratio for each cell type.

Storage

-20°C, 1 year.

Avoid repetitive freeze-thaw cycles.

Components

Components HY-K2014-1 mL HY-K2014-5 mL HY-K2014-10 mL
PEI Transfection Reagent 1 mL 1 mL × 5 1 mL × 10

Documentation

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100 mg

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  • Experimental Validation Results for PEI Transfection Reagent

    PEI Transfection Reagent purchased from MedChemExpress. Usage Cited in: Nature. 2025 Aug;644(8076):537-546. [Abstract]

    hyBE4max-eGFP and sgRNA expressing plasmid (10 μg) were co-transfected into 6 cm dish using PEI Transfection Reagent. After 72 h, SKOV3 transduced with lentiviral vectors encoding PPP2R1A WT and P179R mutant were selected by sorting GFP positive cells.

  • Experimental Validation Results for PEI Transfection Reagent

    PEI Transfection Reagent purchased from MedChemExpress. Usage Cited in: Nat Aging. 2025 Jun;5(6):1097-1113. [Abstract]

    Transfection was carried out using PEI Transfection Reagent for HEK cells. Swedish mutant APP stable cell line 20E2 was cultured and treated with exendin-4 with or without compound C to inhibit AMPK activity. The 20E2 cell line is a Swedish mutant APP695 stable HEK293 cell line cultured in complete DMEM with 50 µg/mL geneticin. For the treatment, HEK cells were transfected in a 6 cm master plate and then split into 4 × 35 mm plates 4 h after transfection, maintained overnight (16 h) before treatment.

  • Experimental Validation Results for PEI Transfection Reagent

    PEI Transfection Reagent purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2025 Aug 29. [Abstract]

    Lenti-CRISPR viruses were generated via triple-plasmid transfection. Briefly, murine LRIG1 sgRNAs were designed with the Synthego CRISPR design tool. The oligos were synthesized, annealed, and cloned and inserted into the LentiCRISPR-LRIG1 vector, followed by Sanger sequencing verification. HEK293T cells were cotransfected with LentiCRISPR-LRIG1 or LentiCRISPR-Control along with the lentiviral second-generation helper plasmids psPAX2 and pCMV-VSV-G via PEI transfection reagent. The culture supernatants harvested at 48 h and 72 h posttransfection were filtered, concentrated with PEG8000 and finally frozen at −80 °C. For transduction, intestinal organoids at a low passage number and 2d $ost-passaging were incubated with pretreated lentivirus (LRIG1−/− or control) in culture medium containing polybrene. The suspension was centrifuged at 37 °C and 1000 ×g for 30 min and incubated at 37 °C for 4 h. After transduction, the organoids were cultured in medium supplemented with 1 μg/mL Puromycin for selection.

Assay Types
  • Experimental Validation Results for PEI Transfection Reagent
    1/16

    MedChemExpress Validation
    Experimental conditions:
    Cell density: 50% of the density during cell transfection
    Dosage: 1 μg of GFP plasmid + 2.5 μL of transfection reagent
    Detection scheme: Detection 48 h after transfection
    The results showed that PEI Transfection Reagent (HY-K2014) showed better transfection performance for GFP plasmid transfection in 293T, CHO, HCT116, HT29, and U2OS cells.

  • Experimental Validation Results for PEI Transfection Reagent
    1/16

    PEI Transfection Reagent purchased from MedChemExpress. Usage Cited in: Nature. 2025 Aug;644(8076):537-546. [Abstract]

    hyBE4max-eGFP and sgRNA expressing plasmid (10 μg) were co-transfected into 6 cm dish using PEI Transfection Reagent. After 72 h, SKOV3 transduced with lentiviral vectors encoding PPP2R1A WT and P179R mutant were selected by sorting GFP positive cells.

  • Experimental Validation Results for PEI Transfection Reagent
    1/16

    PEI Transfection Reagent purchased from MedChemExpress. Usage Cited in: Nat Aging. 2025 Jun;5(6):1097-1113. [Abstract]

    Transfection was carried out using PEI Transfection Reagent for HEK cells. Swedish mutant APP stable cell line 20E2 was cultured and treated with exendin-4 with or without compound C to inhibit AMPK activity. The 20E2 cell line is a Swedish mutant APP695 stable HEK293 cell line cultured in complete DMEM with 50 µg/mL geneticin. For the treatment, HEK cells were transfected in a 6 cm master plate and then split into 4 × 35 mm plates 4 h after transfection, maintained overnight (16 h) before treatment.

  • Experimental Validation Results for PEI Transfection Reagent
    1/16

    PEI Transfection Reagent purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2025 Aug 29. [Abstract]

    Lenti-CRISPR viruses were generated via triple-plasmid transfection. Briefly, murine LRIG1 sgRNAs were designed with the Synthego CRISPR design tool. The oligos were synthesized, annealed, and cloned and inserted into the LentiCRISPR-LRIG1 vector, followed by Sanger sequencing verification. HEK293T cells were cotransfected with LentiCRISPR-LRIG1 or LentiCRISPR-Control along with the lentiviral second-generation helper plasmids psPAX2 and pCMV-VSV-G via PEI transfection reagent. The culture supernatants harvested at 48 h and 72 h posttransfection were filtered, concentrated with PEG8000 and finally frozen at −80 °C. For transduction, intestinal organoids at a low passage number and 2d $ost-passaging were incubated with pretreated lentivirus (LRIG1−/− or control) in culture medium containing polybrene. The suspension was centrifuged at 37 °C and 1000 ×g for 30 min and incubated at 37 °C for 4 h. After transduction, the organoids were cultured in medium supplemented with 1 μg/mL Puromycin for selection.

  • PEI Transfection Reagent Product Image
    1/16
  • Experimental Validation Results for PEI Transfection Reagent
  • Experimental Validation Results for PEI Transfection Reagent
  • Experimental Validation Results for PEI Transfection Reagent
  • Experimental Validation Results for PEI Transfection Reagent
  • PEI Transfection Reagent Product Image