PEI Transfection Reagent

Cat. No.: HY-K2014: Manual COA SDS
FAQs
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MCE PEI Transfection Reagent is designed based on 25 kDa PEI. It has high-efficiency, low-toxicity, strong-stability, and is suitable for many cell types, such as HEK-293、HEK-293T、CHO-K1、COS-1、COS-7、NIH/3T3、Sf9、HepG2 and HeLa et, even some hard-to-transfect cells. It can also be applied to large-scale recombinant protein expression and virus production. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Size	Stock	Quantity
Free Sample (100 μL)	Apply Now
1 mL	In-stock	Estimated Time of Arrival: December 31
5 mL	In-stock	Estimated Time of Arrival: December 31
10 mL	In-stock	Estimated Time of Arrival: December 31

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PEI Transfection Reagent Featured Recommendations:

54 Publications Citing Use of MCE PEI Transfection Reagent

: PEI Transfection Reagent purchased from MCE. Usage Cited in: Nature. 2025 Aug;644(8076):537-546. [Abstract]; hyBE4max-eGFP and sgRNA expressing plasmid (10 μg) were co-transfected into 6 cm dish using PEI Transfection Reagent. After 72 h, SKOV3 transduced with lentiviral vectors encoding PPP2R1A WT and P179R mutant were selected by sorting GFP positive cells.

: PEI Transfection Reagent purchased from MCE. Usage Cited in: Cell Mol Immunol. 2025 Aug 29. [Abstract]; Lenti-CRISPR viruses were generated via triple-plasmid transfection. Briefly, murine LRIG1 sgRNAs were designed with the Synthego CRISPR design tool. The oligos were synthesized, annealed, and cloned and inserted into the LentiCRISPR-LRIG1 vector, followed by Sanger sequencing verification. HEK293T cells were cotransfected with LentiCRISPR-LRIG1 or LentiCRISPR-Control along with the lentiviral second-generation helper plasmids psPAX2 and pCMV-VSV-G via PEI transfection reagent. The culture supernatants harvested at 48 h and 72 h posttransfection were filtered, concentrated with PEG8000 and finally frozen at −80 °C. For transduction, intestinal organoids at a low passage number and 2d $ost-passaging were incubated with pretreated lentivirus (LRIG1^−/− or control) in culture medium containing polybrene. The suspension was centrifuged at 37 °C and 1000 ×g for 30 min and incubated at 37 °C for 4 h. After transduction, the organoids were cultured in medium supplemented with 1 μg/mL Puromycin for selection.

: PEI Transfection Reagent purchased from MCE. Usage Cited in: Nat Aging. 2025 Jun;5(6):1097-1113. [Abstract]; Transfection was carried out using PEI Transfection Reagent for HEK cells. Swedish mutant APP stable cell line 20E2 was cultured and treated with exendin-4 with or without compound C to inhibit AMPK activity. The 20E2 cell line is a Swedish mutant APP695 stable HEK293 cell line cultured in complete DMEM with 50 µg/mL geneticin. For the treatment, HEK cells were transfected in a 6 cm master plate and then split into 4 × 35 mm plates 4 h after transfection, maintained overnight (16 h) before treatment.

: MedChemExpress Validation
Experimental conditions:
Cell density: 50% of the density during cell transfection
Dosage: 1 μg of GFP plasmid + 2.5 μL of transfection reagent
Detection scheme: Detection 48 h after transfection
The results showed that PEI Transfection Reagent (HY-K2014) showed better transfection performance for GFP plasmid transfection in 293T, CHO, HCT116, HT29, and U2OS cells.

Description
Storage
Components
Documentation

Description
& Advantages

PEI is a commonly used polymer carrier in gene transfection. It consists of cationic polymers and can introduce nucleic acids into eukaryotic cells. In polymer-based transfection, exogenous DNA forms complexes with cationic polymers that enter host cells by endocytosis.

MCE PEI Transfection Reagent is designed based on 25 kDa PEI. It is modified by introducing functional genes to enhance the binding ability of DNA, and reduced the cytotoxicity. In addition, the cell uptake and the endosome escape functions of PEI modified was enhanced significantly.

MCE PEI Transfection Reagent is high-efficiency, low-toxicity, strong-stability, and suitable for many cell types, such as HEK-293、HEK-293T、CHO-K1、COS-1、COS-7、NIH/3T3、Sf9、HepG2 and HeLa et, even some hard-to-transfect cells. It can also be applied to large-scale recombinant protein expression and virus production.

Storage

-20°C, 1 year.

Avoid repetitive freeze-thaw cycles.

Components

Components	HY-K2014-1 mL	HY-K2014-5 mL	HY-K2014-10 mL
PEI Transfection Reagent	1 mL	1 mL × 5	1 mL × 10

Documentation

Manual (1082 KB) SDS (177 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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PEI Transfection Reagent

PEI Transfection Reagent Featured Recommendations:

54 Publications Citing Use of MCE PEI Transfection Reagent

Description

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Documentation

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