2. MCE Kits
  3. Protein Biology
  4. Protein Purification Protein Electrophoresis & WB
  5. Binding/Washing Buffer
  6. TBS-T Powder (1 L of 1×)

TBS-T Powder (1 L of 1×) 

Cat. No.: HY-K1025
Manual COA SDS Technical Support
Expression Purification

MCE TBS-T Powder (1 L of 1×) consists of NaCl, KCl, Tris and Tween 20, and is widely used as washing buffer in immunocytochemistry, FISH or ELISA, and can also be used for antibody dilution and preparation of blocking solution. The 20 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Size Price Stock Quantity
Free Sample (2 pouches)   Apply Now  
20 pouches In-stock
100 pouches In-stock
or Bulk Inquiry

* Please select Quantity before adding items.

    TBS-T Powder (1 L of 1×) purchased from MCE. Usage Cited in: Cancer Biother Radiopharm. 2025 Jun;40(5):323-338.  [Abstract]

    Detection of relative expression of KIF4A using Western blot assay.

    TBS-T Powder (1 L of 1×) purchased from MCE. Usage Cited in: Oral Surg Oral Med Oral Pathol Oral Radiol. 2025 Sep 17:S2212-4403(25)01212-X.

    FTH silencing efficiency in SCC15 cells, as assessed Western blot (GAPDH loading control).

    TBS-T Powder (1 L of 1×) purchased from MCE. Usage Cited in: Autophagy. 2023 Jun;19(6):1745-1763.  [Abstract]

    Immunoblotting of the ATG8 protein level in total proteins extracted from transgenic MdATG8i OE#1, OE#7, and WT leaves infected by V. Mali. The non-lipidated and lipidated forms of ATG8 are indicated by ATG8 and ATG8–PE, respectively; hpt, hours post-treatment. Ponceau S staining of the membrane is shown as a loading control.

    • Description

    • Storage

    • Protocol

    • Components

    • Documentation

    Description
    & Advantages

    TBS-T buffer is widely used as washing buffer in immunocytochemistry, FISH or ELISA, and can also be used for antibody dilution and preparation of blocking solution.

    MCE TBS-T Powder (1 L of 1×) consists of NaCl, KCl, Tris and Tween 20. Each pouch can be diluted to 1 L 1× TBS-T buffer, in which the concentration of NaCl is 137 mM, the concentration of KCl is 2.7 mM, the concentration of Tris is 25 mM and the concentration of Tween 20 is 0.05%. This product provides a convenient way to make TBS-T buffer and eliminates the need to weigh and mix individual components.

    Storage

    Store at room temperature for 3 years.

    Protocol

    1. Add 1 pouch of TBS-T Powder into the cleaned beaker, dissolve with 600 mL distilled water under a magnetic stirrer.

    2. Add distilled water to the solution in step 1 until the total volume is 1 L. The final solution is 1× TBS-T buffer.

    Note: The pH of the 1× TBS-T buffer is 7.4 ± 0.15 @ 25°C.

    Components
    Components HY-K1025-20 pouches HY-K1025-100 pouches
    TBS-T Powder (1 L of 1×) 20 pouches 5 × 20 pouches
    Documentation

    Manual (93 KB) SDS (252 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TBS-T Instant GranulesWestern blotWBTBS-T buffer

    Your Recently Viewed Products:

    Bulk Inquiry

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    TBS-T Powder (1 L of 1×)
    Cat. No.:
    HY-K1025
    Quantity:
    MCE Japan Authorized Agent:

    Request for COA Report

    Please fill out this form to request the COA report. We will send it to your email within 3 working days. Your information is safe with us. * Required Fields.

    .

    Product Name

    		  

    Lot # *

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Remarks

    Request for COA Report

    We have received your request and will respond to you as soon as possible.