2. MCE Kits
  3. Protein Biology
  4. Protein Electrophoresis & WB
  5. Chemiluminescent Detection
  6. Ultra High Sensitivity ECL Kit

Ultra High Sensitivity ECL Kit 

Cat. No.: HY-K1005
Manual COA SDS Technical Support
Expression Purification

MCE Ultra High Sensitivity ECL Kit is an ultra-sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) labeled antibodies on western blots. The 100 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

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500 mL In-stock
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165 Publications Citing Use of MCE Ultra High Sensitivity ECL Kit

WB

    Ultra High Sensitivity ECL Kit purchased from MCE. Usage Cited in: Autophagy. 2025 Feb;21(2):352-373.  [Abstract]

    Western blot showing protein levels of LAMP2A, TP53, CDKN1A, and CDKN2A in NPC treated with DMSO, TNF (20 ng/mL, 48 h), or ATRA (10 μM, 48 h).

    Ultra High Sensitivity ECL Kit purchased from MCE. Usage Cited in: Theranostics. 2024 Aug 12;14(12):4874-4893.  [Abstract]

    Evaluation of EV marker protein (Alix, HSP70, TSG01, and Cytc) expression levels.

    Ultra High Sensitivity ECL Kit purchased from MCE. Usage Cited in: Small. 2024 Jan;20(1):e2303425.  [Abstract]

    Western blot analysis of NV-positive markers (Alix, TSG101, and HSP70) (left panel) and proteins (IL-10, SOD2, and VEGF) (right panel) in MSCs and MSC-NVs.

    • Description

    • Storage

    • Protocol

    • Components

    • Documentation

    Description
    & Advantages

    MCE Ultra High Sensitivity ECL Kit enables detection at femtogram range of antigen by oxidizing luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualized on X-ray film or digital imaging systems. MCE Ultra High Sensitivity ECL Kit produces a strong, long-lived signal, which, combined with very low background levels, allows for long exposure times enabling the detection of low-abundance proteins.

    Storage

    Stored at 4°C, and is stable for 1 year.

    Protect from light.
    Protocol

    1. Take MCE Ultra High Sensitivity ECL Kit out of the refrigerator and allow it to equilibrate at room temperature for about 20 minutes.

    2. Prepare the ECL working solution.

    Note: Approximately 0.1 mL of ECL Reagent is required per cm2 of membrane area.

    a). Mix solutions A and B in a 1:1 ratio. Use a in sufficient volume to ensure that the blot is completely wetted with the ECL working solution.

    b). It is recommended to prepare the working solution just before use, although the mixed reagent solution is stable for several hours at room temperature.

    3. Place the blot, protein-side up, in a clean container, and add ECL working solution.

    4. Incubate the blot for 1-2 minutes at room temperature.

    5. Drain the excess substrate and place the blot in a plastic membrane protector. Remove all air bubbles between the blot and the surface of the membrane protector.

    6. Visualize the blot using X-ray film or a CCD-based imaging system.
    Components
    Components HY-K1005-100 mL HY-K1005-500 mL
    Solution A 50 mL 50 mL × 5
    Solution B 50 mL 50 mL ×5
    Documentation

    Manual (160 KB) SDS (251 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Super ECL Detection Reagentfluorescent ECL detection reagentWestern blotting developer

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