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Ultra High Sensitivity ECL Kit

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MCE Ultra High Sensitivity ECL Kit is an ultra-sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) labeled antibodies on western blots. The 100 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

  • Storage :

    Stored at 4°C, and is stable for 1 year.

    Protect from light.

  • Experimental Validation Results for Ultra High Sensitivity ECL Kit
  • Experimental Validation Results for Ultra High Sensitivity ECL Kit
  • Experimental Validation Results for Ultra High Sensitivity ECL Kit
  • Ultra High Sensitivity ECL Kit Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Description & Advantages

MCE Ultra High Sensitivity ECL Kit enables detection at femtogram range of antigen by oxidizing luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualized on X-ray film or digital imaging systems. MCE Ultra High Sensitivity ECL Kit produces a strong, long-lived signal, which, combined with very low background levels, allows for long exposure times enabling the detection of low-abundance proteins.

Protocol

1. Take MCE Ultra High Sensitivity ECL Kit out of the refrigerator and allow it to equilibrate at room temperature for about 20 minutes.

2. Prepare the ECL working solution.

Note: Approximately 0.1 mL of ECL Reagent is required per cm2 of membrane area.

a). Mix solutions A and B in a 1:1 ratio. Use a in sufficient volume to ensure that the blot is completely wetted with the ECL working solution.

b). It is recommended to prepare the working solution just before use, although the mixed reagent solution is stable for several hours at room temperature.

3. Place the blot, protein-side up, in a clean container, and add ECL working solution.

4. Incubate the blot for 1-2 minutes at room temperature.

5. Drain the excess substrate and place the blot in a plastic membrane protector. Remove all air bubbles between the blot and the surface of the membrane protector.

6. Visualize the blot using X-ray film or a CCD-based imaging system.

Storage

Stored at 4°C, and is stable for 1 year.

Protect from light.

Components

Components HY-K1005-100 mL HY-K1005-500 mL
Solution A 50 mL 50 mL × 5
Solution B 50 mL 50 mL ×5

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  • Experimental Validation Results for Ultra High Sensitivity ECL Kit
  • Experimental Validation Results for Ultra High Sensitivity ECL Kit
  • Experimental Validation Results for Ultra High Sensitivity ECL Kit
  • Ultra High Sensitivity ECL Kit Product Image