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  6. Protein A/G Magnetic Beads

Protein A/G Magnetic Beads 

Cat. No.: HY-K0202
Manual COA SDS Technical Support

MCE Protein A/G Magnetic Beads provide a fast and convenient method for Immunoprecipitation, Co-Immunoprecipitation and Chromatin Immunoprecipitation.

Protein A/G Magnetic Beads
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Protein A/G Magnetic Beads Featured Recommendations:

733 Publications Citing Use of MCE Protein A/G Magnetic Beads

IP

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Mar;20(3):292-304.  [Abstract]

    Immunoblot analysis of protein immunoprecipitation (IP) with antibodies against FXYD3 or TRAF3 in HaCaT cells treated with IL-17A (100 ng/ml).

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cell Res. 2025 Jan 29.  [Abstract]

    Immunoprecipitation (IP) analysis of binding between Raptin and GRM3 VFT or transmembrane domain (TMD) of GRM3 in hypothalamic GT1-7 neurons. The cell lysate of hypothalamic GT1-7 neurons with human GRM3-VFT or human GRM3-TMD overexpression was incubated with PBS or His-Raptin.

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2024 Oct 14.  [Abstract]

    Protein A/G Magnetic Beads (4 h). The interaction between LMP1‐GFP and ALIX‐MYC was detected by a co‐IP assay.

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Sep 26;9(1):253.  [Abstract]

    Protein A/G Magnetic Beads (30 µL) were conjugated with antibodies and lysis buffer at 4 °C. Later, the protein samples were incubated with preconjugated beads overnight at 4 °C on a rotating wheel.
    Coimmunoprecipitation of GRP75, ANT2, and UCP1. HEK293T cells were co-transfected with Myc-GRP75, ANT2, and Flag-UCP1. Protein extracts were immunoprecipitated with antibodies against Myc-tag or Flag-tag, followed by immunoblotting with the indicated antibodies.

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 Apr 2.  [Abstract]

    Protein A/G Magnetic Beads were washed four times with 0.5% PBS‐Triton X‐100. The antibody solution was then added, and the magnetic beads and antibodies were incubated for 1 h at 25°C on a flip mixer. The antibody‐conjugated magnetic beads were washed four times with 0.5% PBS‐Triton X‐100. Prepared protein supernatant was then added, and the antibodies and proteins conjugated to the magnetic beads were incubated for 2 h at 4°C on a flip mixer. After washing with 0.5% PBS‐Triton X‐100, 1 × SDS‐P

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 Mar 1.  [Abstract]

    The cell lysate extracted from ccRCC cells was incubated in Protein A/G Magnetic Beads with primary antibodies or IgG overnight at 4°C with shaking. The immune complexes were incubated with protein A/G magnetic beads for 2 h at room temperature followed by washing with PBST (PBS + 0.05% Tween 20) to remove the unbound immune complexes.
    (H) The DBT/YAP‐ANXA2 interaction was determined by co‐IP assays in A498 and CAKI‐1 cells with Myc‐ANXA2 overexpression.

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 Feb 28.  [Abstract]

    Cells lysates were prepared by using NP40 lysis buffer containing PMSF and phosphatase inhibitor cocktail. Anti‐SHP1 (1:100) or anti‐Flag (1:100) were pre‐mixed with Protein A/G Magnetic Beads for 2 h at 4°C.
    Co‐IP analysis of SHP1‐interacted STING and TRAF6 under different treatment regimens

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2021 Dec;41(12):1354-1372.  [Abstract]

    Immunoprecipitation of MAGE‐C3 and IFNGR1 from digitonin lysates in KYSE30 cells with or without IFN‐γ.

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Nat Metab. 2024 Aug 12.  [Abstract]

    Protein A/G Magnetic Beads (50 μL; 2 h). Immunoblotting shows Kbhb or Kac of ALDOB in livers from male mice fed a control diet or KD for 5 weeks. Immunoprecipitation was performed with a pan-Kbhb or a pan-Kac antibody from mouse liver whole-cell lysates.

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Neuro Oncol. 2024 Jan 5;26(1):137-152.  [Abstract]

    The supernatants were immunoprecipitated with antibodies prebound with Protein A/G Magnetic Beads.
    Co-IP between ADAM22 and ITGB1 in GH3 and AtT20 cells.

    MCE Biological Validation

    IP

      Protein A/G Magnetic Beads purchased from MCE. Usage Cited in: Signal Transduct Target Ther. 2024 Sep 26;9(1):253.  [Abstract]

      Customer Validation
      Protein A/G Magnetic Beads (30 µL).Coimmunoprecipitation of GRP75, ANT2, and UCP1. HEK293T cells were co-transfected with Myc-GRP75, ANT2, and Flag-UCP1. Protein extracts were immunoprecipitated with antibodies against Myc-tag or Flag-tag, followed by immunoblotting with the indicated antibodies.

      Protein A/G Magnetic Beads purchased from MCE. Usage Cited in: Cell Res. 2025 Jan 29.  [Abstract]

      Customer Validation
      Immunoprecipitation (IP) analysis of binding between Raptin and GRM3 VFT or transmembrane domain (TMD) of GRM3 in hypothalamic GT1-7 neurons. The cell lysate of hypothalamic GT1-7 neurons with human GRM3-VFT or human GRM3-TMD overexpression was incubated with PBS or His-Raptin.

      Protein A/G Magnetic Beads purchased from MCE. Usage Cited in: Cancer Commun (Lond). 2024 Oct 14.  [Abstract]

      Customer Validation
      Protein A/G Magnetic Beads (4 h). The interaction between LMP1‐GFP and ALIX‐MYC was detected by a co‐IP assay.

      Protein A/G Magnetic Beads purchased from MCE. Usage Cited in: Cancer Commun (Lond). 2023 Apr 2.  [Abstract]

      Customer Validation
      MDA‐MB‐231 and SKBRS parent cell lysates were immunoprecipitated with anti‐YAP1, anti‐VPS4B, or anti‐CHMP2B antibodies, and YAP1 binding to VPS4B and CHMP2B was examined with WB analysis.

      Protein A/G Magnetic Beads purchased from MCE. Usage Cited in: Cancer Commun (Lond). 2023 Mar 1.  [Abstract]

      Customer Validation
      The cell lysate extracted from ccRCC cells was incubated in Protein A/G Magnetic Beads with primary antibodies or IgG overnight at 4°C with shaking. The immune complexes were incubated with protein A/G magnetic beads for 2 h at room temperature followed by washing with PBST (PBS + 0.05% Tween 20) to remove the unbound immune complexes.
      (H) The DBT/YAP‐ANXA2 interaction was determined by co‐IP assays in A498 and CAKI‐1 cells with Myc‐ANXA2 overexpression.

      • Description

      • Storage

      • Protocol

      • Components

      • Documentation

      Description
      & Advantages

      Protein A/G Magnetic Beads provide a fast and convenient method for magnetic isolation of proteins using affinity binding. MCE Protein A/G Magnetic Beads are typically used for isolating antibodies from serum, cell culture supernatant or ascites and for Immunoprecipitation and Co-Immunoprecipitation of antigens from cell or tissue extracts.

      1. During immunoprecipitation, only a small amount of magnetic beads are needed.

      2. onvenient and time saving.

      3. Low non-specific binding.

      4. Minimal sample loss.

      5. Antibody binding capacity up to 0.7 mg/mL.

      6. Stable, one bottle solution.
      Storage

      4°C, 2 years.

      Do not centrifuge, dry or freeze the magnetic beads.
      Protocol

      1. Preparation of Magnetic Beads

      1.1 Resuspend the Magnetic Beads in the vial (tilt and rotate for 2 minutes or gently pipette for 10 times).

      1.2 Transfer 25-50 μL of Protein A/G Magnetic Beads into a 1.5 mL tube (Transfer amount may be adjusted as required).

      1.3 Add 400 μL of binding/wash buffer to the beads and gently pipette to mix. Place the tube into a magnetic stand to collect the beads against the side of the tube (Hereinafter referred to as magnetic separation). Remove and discard the supernatant. Repeat this step for 2 times.

      2. Binding of Antibody

      2.1 Dilute antibody (Ab) to the final concentration of 5-50 μg/mL with binding/wash buffer. The optimal amount of Ab may be adjusted as required.

      2.2 Add 400 μL of diluted Ab to the Protein A/G Magnetic Beads. Rotate tube for 30 minutes at room temperature or 2 hours at 4°C.

      2.3 Perform magnetic separation. Transfer the supernatant into a new tube for further analysis, if desired. The supernatant is the non-binding fraction.

      2.4 Add 400 μL of binding/wash buffer to the beads and gently pipette to mix. Place the tube into a magnetic stand to collect the beads against the side of the tube. Remove and discard the supernatant. Repeat this step for 4 times.

      3. Immunoprecipitation of Target Antigen

      3.1 Remove the tubes from the magnetic separator and add your sample containing the antigen (Ag) (typically 5-50 μg in 400 μL binding/wash buffer) and gently pipette to resuspend the Protein A/G Magnetic Beads-Ab complex.

      3.2 Incubate with rotation for 30 minutes at room temperature or 2 hours at 4°C to allow Ag to bind to the Protein A/G Magnetic Beads-Ab complex.

      3.3 Perform magnetic separation. Remove and discard the supernatant.

      3.4 Wash the Magbeads-Ab-Ag complex 5 times using 400 μL binding/wash buffer for each wash. Perform magnetic separation between each wash, remove supernatant and resuspend by gentle pipetting.

      3.5 Resuspend the Protein A/G Magnetic Beads-Ab-Ag complex in 400 μL binding/wash buffer and transfer the bead suspension into a clean tube. This is recommended to avoid co-elution of the proteins bound to the tube wall.

      4. Elution

      This is a non-denaturation elution method.

      4.1 Perform magnetic separation and remove the supernatant. Add 400 μL of binding/wash buffer into the tube and rotate for 5 minutes. Perform magnetic separation for 1 minute and remove the supernatant. Then add 25-50 μL elution buffer into the tube with magnetic beads-Ab-Ag complex, rotate for 5 minutes.

      4.2 Perform magnetic separation, collect the supernatant.

      4.3 The final solution can be used as samples for denaturing SDS-PAGE. Or the elution can be adjusted to neutral pH with neutralization buffer immediately and used for further analysis.
      Components
      Components HY-K0202-1 mL HY-K0202-5 mL
      Protein A/G Magnetic Beads 1 mL 1 mL × 5
      Documentation

      Manual (246 KB) SDS (108 KB) COA (142 KB)
      Help & FAQs
      • Do most proteins show cross-species activity?

        Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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