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Protein A/G Magnetic Beads

1434 Cited Publications
Customer Review

Based on 1434 publication(s) in Google Scholar

MCE Protein A/G Magnetic Beads provide a fast and convenient method for Immunoprecipitation, Co-Immunoprecipitation and Chromatin Immunoprecipitation. The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

  • Storage :
    4°C, 2 years. Do not centrifuge, dry or freeze the magnetic beads.
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Protein A/G Magnetic Beads Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Description & Advantages

Protein A/G Magnetic Beads provide a fast and convenient method for magnetic isolation of proteins using affinity binding. MCE Protein A/G Magnetic Beads are typically used for isolating antibodies from serum, cell culture supernatant or ascites and for Immunoprecipitation and Co-Immunoprecipitation of antigens from cell or tissue extracts.

1. During immunoprecipitation, only a small amount of magnetic beads are needed.

2. onvenient and time saving.

3. Low non-specific binding.

4. Minimal sample loss.

5. Antibody binding capacity up to 0.7 mg/mL.

6. Stable, one bottle solution.

Protocol

1. Preparation of Magnetic Beads

1.1 Resuspend the Magnetic Beads in the vial (tilt and rotate for 2 minutes or gently pipette for 10 times).

1.2 Transfer 25-50 μL of Protein A/G Magnetic Beads into a 1.5 mL tube (Transfer amount may be adjusted as required).

1.3 Add 400 μL of binding/wash buffer to the beads and gently pipette to mix. Place the tube into a magnetic stand to collect the beads against the side of the tube (Hereinafter referred to as magnetic separation). Remove and discard the supernatant. Repeat this step for 2 times.

2. Binding of Antibody

2.1 Dilute antibody (Ab) to the final concentration of 5-50 μg/mL with binding/wash buffer. The optimal amount of Ab may be adjusted as required.

2.2 Add 400 μL of diluted Ab to the Protein A/G Magnetic Beads. Rotate tube for 30 minutes at room temperature or 2 hours at 4°C.

2.3 Perform magnetic separation. Transfer the supernatant into a new tube for further analysis, if desired. The supernatant is the non-binding fraction.

2.4 Add 400 μL of binding/wash buffer to the beads and gently pipette to mix. Place the tube into a magnetic stand to collect the beads against the side of the tube. Remove and discard the supernatant. Repeat this step for 4 times.

3. Immunoprecipitation of Target Antigen

3.1 Remove the tubes from the magnetic separator and add your sample containing the antigen (Ag) (typically 5-50 μg in 400 μL binding/wash buffer) and gently pipette to resuspend the Protein A/G Magnetic Beads-Ab complex.

3.2 Incubate with rotation for 30 minutes at room temperature or 2 hours at 4°C to allow Ag to bind to the Protein A/G Magnetic Beads-Ab complex.

3.3 Perform magnetic separation. Remove and discard the supernatant.

3.4 Wash the Magbeads-Ab-Ag complex 5 times using 400 μL binding/wash buffer for each wash. Perform magnetic separation between each wash, remove supernatant and resuspend by gentle pipetting.

3.5 Resuspend the Protein A/G Magnetic Beads-Ab-Ag complex in 400 μL binding/wash buffer and transfer the bead suspension into a clean tube. This is recommended to avoid co-elution of the proteins bound to the tube wall.

4. Elution

This is a non-denaturation elution method.

4.1 Perform magnetic separation and remove the supernatant. Add 400 μL of binding/wash buffer into the tube and rotate for 5 minutes. Perform magnetic separation for 1 minute and remove the supernatant. Then add 25-50 μL elution buffer into the tube with magnetic beads-Ab-Ag complex, rotate for 5 minutes.

4.2 Perform magnetic separation, collect the supernatant.

4.3 The final solution can be used as samples for denaturing SDS-PAGE. Or the elution can be adjusted to neutral pH with neutralization buffer immediately and used for further analysis.

Storage

4°C, 2 years.

Do not centrifuge, dry or freeze the magnetic beads.

Components

Components HY-K0202-1 mL HY-K0202-5 mL
Protein A/G Magnetic Beads 1 mL 1 mL × 5

Documentation

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  • Experimental Validation Results for Protein A/G Magnetic Beads

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cell Res. 2025 Mar;35(3):165-185. [Abstract]

    Immunoprecipitation (IP) analysis of binding between Raptin and GRM3 VFT or transmembrane domain (TMD) of GRM3 in hypothalamic GT1-7 neurons. The cell lysate of hypothalamic GT1-7 neurons with human GRM3-VFT or human GRM3-TMD overexpression was incubated with PBS or His-Raptin.

  • Experimental Validation Results for Protein A/G Magnetic Beads

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Sep 26;9(1):253. [Abstract]

    Protein A/G Magnetic Beads (30 µL) were conjugated with antibodies and lysis buffer at 4 °C. Later, the protein samples were incubated with preconjugated beads overnight at 4 °C on a rotating wheel.
    Coimmunoprecipitation of GRP75, ANT2, and UCP1. HEK293T cells were co-transfected with Myc-GRP75, ANT2, and Flag-UCP1. Protein extracts were immunoprecipitated with antibodies against Myc-tag or Flag-tag, followed by immunoblotting with the indicated antibodies.

  • Experimental Validation Results for Protein A/G Magnetic Beads

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2024 Dec;44(12):1391-1413. [Abstract]

    Protein A/G Magnetic Beads (4 h). The interaction between LMP1‐GFP and ALIX‐MYC was detected by a co‐IP assay.

  • Experimental Validation Results for Protein A/G Magnetic Beads

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Nat Metab. 2024 Aug;6(8):1505-1528. [Abstract]

    Protein A/G Magnetic Beads (50 μL; 2 h). Immunoblotting shows Kbhb or Kac of ALDOB in livers from male mice fed a control diet or KD for 5 weeks. Immunoprecipitation was performed with a pan-Kbhb or a pan-Kac antibody from mouse liver whole-cell lysates.

  • Experimental Validation Results for Protein A/G Magnetic Beads

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Neuro Oncol. 2024 Jan 5;26(1):137-152. [Abstract]

    The supernatants were immunoprecipitated with antibodies prebound with Protein A/G Magnetic Beads.
    Co-IP between ADAM22 and ITGB1 in GH3 and AtT20 cells.

  • Experimental Validation Results for Protein A/G Magnetic Beads

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 Apr;43(4):480-502. [Abstract]

    The cell lysate extracted from ccRCC cells was incubated in Protein A/G Magnetic Beads with primary antibodies or IgG overnight at 4°C with shaking. The immune complexes were incubated with protein A/G magnetic beads for 2 h at room temperature followed by washing with PBST (PBS + 0.05% Tween 20) to remove the unbound immune complexes.
    (H) The DBT/YAP‐ANXA2 interaction was determined by co‐IP assays in A498 and CAKI‐1 cells with Myc‐ANXA2 overexpression.

  • Experimental Validation Results for Protein A/G Magnetic Beads

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 May;43(5):582-612. [Abstract]

    Protein A/G Magnetic Beads were washed four times with 0.5% PBS‐Triton X‐100. The antibody solution was then added, and the magnetic beads and antibodies were incubated for 1 h at 25°C on a flip mixer. The antibody‐conjugated magnetic beads were washed four times with 0.5% PBS‐Triton X‐100. Prepared protein supernatant was then added, and the antibodies and proteins conjugated to the magnetic beads were incubated for 2 h at 4°C on a flip mixer. After washing with 0.5% PBS‐Triton X‐100, 1 × SDS‐P

  • Experimental Validation Results for Protein A/G Magnetic Beads

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 Apr;43(4):435-454. [Abstract]

    Cells lysates were prepared by using NP40 lysis buffer containing PMSF and phosphatase inhibitor cocktail. Anti‐SHP1 (1:100) or anti‐Flag (1:100) were pre‐mixed with Protein A/G Magnetic Beads for 2 h at 4°C.
    Co‐IP analysis of SHP1‐interacted STING and TRAF6 under different treatment regimens

  • Experimental Validation Results for Protein A/G Magnetic Beads

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Mar;20(3):292-304. [Abstract]

    Immunoblot analysis of protein immunoprecipitation (IP) with antibodies against FXYD3 or TRAF3 in HaCaT cells treated with IL-17A (100 ng/ml).

  • Experimental Validation Results for Protein A/G Magnetic Beads

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2021 Dec;41(12):1354-1372. [Abstract]

    Immunoprecipitation of MAGE‐C3 and IFNGR1 from digitonin lysates in KYSE30 cells with or without IFN‐γ.

Assay Types
  • Experimental Validation Results for Protein A/G Magnetic Beads
    1/16

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cell Res. 2025 Mar;35(3):165-185. [Abstract]

    Immunoprecipitation (IP) analysis of binding between Raptin and GRM3 VFT or transmembrane domain (TMD) of GRM3 in hypothalamic GT1-7 neurons. The cell lysate of hypothalamic GT1-7 neurons with human GRM3-VFT or human GRM3-TMD overexpression was incubated with PBS or His-Raptin.

  • Experimental Validation Results for Protein A/G Magnetic Beads
    1/16

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Sep 26;9(1):253. [Abstract]

    Protein A/G Magnetic Beads (30 µL) were conjugated with antibodies and lysis buffer at 4 °C. Later, the protein samples were incubated with preconjugated beads overnight at 4 °C on a rotating wheel.
    Coimmunoprecipitation of GRP75, ANT2, and UCP1. HEK293T cells were co-transfected with Myc-GRP75, ANT2, and Flag-UCP1. Protein extracts were immunoprecipitated with antibodies against Myc-tag or Flag-tag, followed by immunoblotting with the indicated antibodies.

  • Experimental Validation Results for Protein A/G Magnetic Beads
    1/16

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2024 Dec;44(12):1391-1413. [Abstract]

    Protein A/G Magnetic Beads (4 h). The interaction between LMP1‐GFP and ALIX‐MYC was detected by a co‐IP assay.

  • Experimental Validation Results for Protein A/G Magnetic Beads
    1/16

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Nat Metab. 2024 Aug;6(8):1505-1528. [Abstract]

    Protein A/G Magnetic Beads (50 μL; 2 h). Immunoblotting shows Kbhb or Kac of ALDOB in livers from male mice fed a control diet or KD for 5 weeks. Immunoprecipitation was performed with a pan-Kbhb or a pan-Kac antibody from mouse liver whole-cell lysates.

  • Experimental Validation Results for Protein A/G Magnetic Beads
    1/16

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Neuro Oncol. 2024 Jan 5;26(1):137-152. [Abstract]

    The supernatants were immunoprecipitated with antibodies prebound with Protein A/G Magnetic Beads.
    Co-IP between ADAM22 and ITGB1 in GH3 and AtT20 cells.

  • Experimental Validation Results for Protein A/G Magnetic Beads
    1/16

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 Apr;43(4):480-502. [Abstract]

    The cell lysate extracted from ccRCC cells was incubated in Protein A/G Magnetic Beads with primary antibodies or IgG overnight at 4°C with shaking. The immune complexes were incubated with protein A/G magnetic beads for 2 h at room temperature followed by washing with PBST (PBS + 0.05% Tween 20) to remove the unbound immune complexes.
    (H) The DBT/YAP‐ANXA2 interaction was determined by co‐IP assays in A498 and CAKI‐1 cells with Myc‐ANXA2 overexpression.

  • Experimental Validation Results for Protein A/G Magnetic Beads
    1/16

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 May;43(5):582-612. [Abstract]

    Protein A/G Magnetic Beads were washed four times with 0.5% PBS‐Triton X‐100. The antibody solution was then added, and the magnetic beads and antibodies were incubated for 1 h at 25°C on a flip mixer. The antibody‐conjugated magnetic beads were washed four times with 0.5% PBS‐Triton X‐100. Prepared protein supernatant was then added, and the antibodies and proteins conjugated to the magnetic beads were incubated for 2 h at 4°C on a flip mixer. After washing with 0.5% PBS‐Triton X‐100, 1 × SDS‐P

  • Experimental Validation Results for Protein A/G Magnetic Beads
    1/16

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2023 Apr;43(4):435-454. [Abstract]

    Cells lysates were prepared by using NP40 lysis buffer containing PMSF and phosphatase inhibitor cocktail. Anti‐SHP1 (1:100) or anti‐Flag (1:100) were pre‐mixed with Protein A/G Magnetic Beads for 2 h at 4°C.
    Co‐IP analysis of SHP1‐interacted STING and TRAF6 under different treatment regimens

  • Experimental Validation Results for Protein A/G Magnetic Beads
    1/16

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Mar;20(3):292-304. [Abstract]

    Immunoblot analysis of protein immunoprecipitation (IP) with antibodies against FXYD3 or TRAF3 in HaCaT cells treated with IL-17A (100 ng/ml).

  • Experimental Validation Results for Protein A/G Magnetic Beads
    1/16

    Protein A/G Magnetic Beads purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2021 Dec;41(12):1354-1372. [Abstract]

    Immunoprecipitation of MAGE‐C3 and IFNGR1 from digitonin lysates in KYSE30 cells with or without IFN‐γ.

  • Protein A/G Magnetic Beads Product Image
    1/16
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Experimental Validation Results for Protein A/G Magnetic Beads
  • Protein A/G Magnetic Beads Product Image