Restriction Endonuclease

MCE Restriction Endonucleases are a class of high-performance enzymatic reagents prepared via genetic engineering recombination technology, specifically designed for the precise modification and construction of plasmid DNA, PCR products, and genomic DNA. While maintaining high fidelity and specificity, this product series enables rapid digestion, significantly reducing experimental operation time. It effectively minimizes the risk of star activity, ensuring clear and reliable cleavage results under mild reaction conditions.

Cat. No.: HY-KE7001 ApaL I

MCE ApaL I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Alw44 I, Vne I.

Cat. No.: HY-KE7006 Bgl II

MCE Bgl II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7007 Bsa I

MCE Bsa I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Eco31 I, Bso31 I, BspTN I.

Cat. No.: HY-KE7008 BstB I

MCE BstB I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Asu II, Bpu14 I, Bsp119 I, BspT104 I, Nsp V, Sfu I.

Cat. No.: HY-KE7010 Cla I

MCE Cla I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: BspD I, Bsa29 I, BseC I, Bsu15 I, BsuTU I.

Cat. No.: HY-KE7009 BstE II

MCE BstE II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: BstP I, Eco91 I, EcoO65 I, PspE I.

Cat. No.: HY-KE7003 Asc I

MCE Asc I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: PalA I, Sgs I.

Cat. No.: HY-KE7002 Avr II

MCE Avr II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: AspA2 I, Bln I, XmaJ I.

Cat. No.: HY-KE7004 BamH I

MCE BamH I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7005 Bcl I

MCE Bcl I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Fba I, Ksp22 I.

Cat. No.: HY-KE7045 Xho I

Xho I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: PaeR7 I, Sfr274 I, Sla I.

Cat. No.: HY-KE7046 Nru I

Nru I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Bsp68 I, BtuM I, Rru I.

Cat. No.: HY-KE7047 Age I

Age I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: AsiG I, CspA I, BshT I, PinA I.

Cat. No.: HY-KE7048 BspQ I

BspQ I is one of the endonucleases of Type IIs that recognize non-palindromic sequences and cut outside of the recognition sequence, and is commonly used in Golden Gate assembly. Isoschizomers: Sap I, Lgu I, PciS I.

Cat. No.: HY-KE7049 Sge I

Sge I restriction enzyme recognizes and cleaves DNA targets containing 5-methylcytosine on one or both DNA strands, and cuts best at 37℃.

Cat. No.: HY-KE7050 BsmB I

BsmB I is one of the endonucleases of Type IIs that recognize non-palindromic sequences and cut outside of the recognition sequence, and is commonly used in Golden Gate assembly. Isoschizomers: Esp3 I, BstGZ53 I, Esp16 I, Esp23 I.

Cat. No.: HY-KE7052 BsmB I, ADCF

BsmB I, ADCF is animal derived component free, and has good redundancy of enzyme activity, which can easily cope with excessive substrate or difficult template enzyme cutting. Isoschizomers: Esp3 I, BstGZ53 I, Esp16 I, Esp23 I.

Cat. No.: HY-KE7053 EcoR I, ADCF

EcoR I, ADCF is animal derived component free, and has good redundancy of enzyme activity, which can easily cope with excessive substrate or difficult template enzyme cutting. Isoschizomers: Fun II.

Cat. No.: HY-KE7054 Xba I, ADCF

Xba I, ADCF is animal derived component free, and has good redundancy of enzyme activity, which can easily cope with excessive substrate or difficult template enzyme cutting.

Cat. No.: HY-KE7011 Dpn I

MCE Dpn I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Mal I.

Cat. No.: HY-KE7012 Dpn II

MCE Dpn II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Mbo I, Sau3A I, Bsp143 I, BstMB I, Nde II, BstKT I.

Cat. No.: HY-KE7013 Eag I

MCE Eag I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: BseX3 I, BstZ I, EclX I, Eco52 I.

Cat. No.: HY-KE7014 EcoR I

MCE EcoR I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Fun II.

Cat. No.: HY-KE7015 EcoR V

MCE EcoR V is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Eco32 I.

Cat. No.: HY-KE7016 Esp3 I

MCE Esp3 I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: BsmB I.

Cat. No.: HY-KE7017 Fsp I

MCE Fsp I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Acc16 I，Nsb I.

Cat. No.: HY-KE7018 Hind III

MCE Hind III is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7019 Hinf I

MCE Hinf I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7020 Hpa I

MCE Hpa I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: KspA I.

Cat. No.: HY-KE7024 Mnl I

MCE Mnl I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7025 Nco I

MCE Nco I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Bsp19 I.

Cat. No.: HY-KE7026 Nde I

MCE Nde I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: FauND I.

Cat. No.: HY-KE7027 Nhe I

MCE Nhe I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Bmt I.

Cat. No.: HY-KE7028 Not I

MCE Not I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: CciN I.

Cat. No.: HY-KE7022 Kpn I

MCE Kpn I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Asp718 I, Acc65 I.

Cat. No.: HY-KE7023 Mlu I

MCE Mlu I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7029 Nsi I

MCE Nsi I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: EcoT22 I, Mph1103 I, Zsp2 I.

Cat. No.: HY-KE7030 Pac I

MCE Pac I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7031 Pst I

MCE Pst I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: BspMA I.

Cat. No.: HY-KE7032 Pvu II

MCE Pvu II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7033 Sac I

MCE Sac I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Psp124B I, Sst I, Ecl136 II, EcoICR I.

Cat. No.: HY-KE7034 Sac II

MCE Sac II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Cfr42 I, Ksp I, Sfr303 I, SgrB I.

Cat. No.: HY-KE7035 Sal I

MCE Sal I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7036 Sbf I

MCE Sbf I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Sda I, Sse8387 I.

Cat. No.: HY-KE7037 Sfi I

MCE Sfi I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7038 Sma I

MCE Sma I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Cfr9 I, Xma I.

Cat. No.: HY-KE7039 Spe I

MCE Spe I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Ahl I, Bcu I.

Cat. No.: HY-KE7040 Sph I

MCE Sph I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Pae I.

Cat. No.: HY-KE7041 Ssp I

MCE Ssp I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7042 Stu I

MCE Stu I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Eco147 I, Pce I, SseB I.

Cat. No.: HY-KE7043 Taq I

Taq I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7044 Xba I

Xba I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7057 O-Glycosidase

O-Glycosidase is highly specific and can release Galβ1-3GalNAc from serine, threonine residues or as part of a glycopeptide or glycoprotein. Applied to glycoprotein biosynthesis analysis, O-glycan bioactive protein O-glycosylation detection and binding site analysis.

Cat. No.: HY-KE7056 RNase III

RNase III is a specific exonuclease expressed in E.coli that can cleave dsRNA into 12-15 bp dsRNA fragments.

Cat. No.: HY-KE7055 RNase R

RNase R is a magnesium ion-dependent 3'-5' exoribonuclease that can increase the abundance of circular RNA by degrading linear RNA in mixed RNA, thereby enriching circular RNA.

Cat. No.: HY-KE7058 RNase H

RNase H is an endoribonuclease expressed and purified from Escherichia coli and can specifically hydrolyze RNA in DNA-RNA hybrid strands.

Cat. No.: HY-KE7066 FnCas12a

FnCas12a(Cpf1), is an RNA-guided, DNA-editable recombinant endonuclease that can be used for gene editing and detection.

Cat. No.: HY-KE7067 Lambda DNA

Lambda DNA (λ DNA) is the DNA in λ phages and is used as a substrate for restriction endonucleases. This product is methylated Lambda DNA.

Cat. No.: HY-KE8012 Protein Deglycosylation Kit(for O-linked Glycans)

Protein Deglycosylation Kit (for O-linked Glycans)contains O-Glycosidase and a2-3,6,8,9 Neuraminidase.Both enzymes are crucial tool enzymes for glycosylation research and are used in combination to enzymatically release O-linked glycans from glycoproteins.O-Glycosidase is capable of cleaving and releasing Core 1 (Gal-B(1-3)-GalNAc-)and Core 3(GIcNAc-B(1-3)-GalNAc-)O-linked disaccharides,which are linked to the hydroxyl groups of Ser or Thr residues in glycoproteins.a2-3,6,8,9 Neuraminidase can remove a(2,3)-,a(2,6)-,a(2,8)-,and a(2,9)-sialic acid residues from the non-reducing termini of O-linked glycans.

Cat. No.: HY-KE8014 Protein Deglycosylation Kit (for N-linked & Complex O-linked Glycans)

Protein Deglycosylation Kit (for N-linked & Complex O-linked Glycans) includes the enzymes and reagents reuired for the removal of all N-linked and some complex O-linked glycans. The enzyme reagent in this kit is a mixture of five glycosidases, namely PNGase F, O-Glycosidase, α2-3,6,8,9 Neuraminidase, β1-4 Galactosidase, and β-N-Acetylhexosaminidase. All enzymes in this kit are recombinant enzymes expressed in Escherichia coli BL21 and subsequently purified. The molecular weights of these enzymes are as follows: PNGase F is 36 kDa, O-Glycosidase is 14 kDa,α2-3,6,8,9 Neuraminidase is 66 kDa, β1-4 Galactosidase is 94 kDa, and β-N-Acetylhexosaminidase is 55 kDa.