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  2. Molecular Biology
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  4. Ligase
  5. T4 DNA Ligase (Fast)

T4 DNA Ligase (Fast) 

Cat. No.: HY-K1049
Manual SDS

MCE T4 DNA Ligase (Fast) is produced by Escherichia coli carrying a T4 phage, catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

T4 DNA Ligase (Fast)
Size Price Stock Quantity
1000 U USD 82 In-stock

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  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

MCE T4 DNA Ligase (Fast) is produced by Escherichia coli carrying a T4 phage, catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme joins DNA fragments with either cohesive or blunt termini, repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, but it is inactive against single-stranded nucleic acids. It is suitable for labeling RNA 3'-ends, cyclizing RNA and DNA oligonucleotides and cloning cDNA. The T4 DNA Ligase requires ATP as a cofactor, completes viscous end connections in just 10 min at room temperature. Simple handling for fast and efficient connections.

Storage

-20℃

1 year

Components
Components HY-K1049-1000U
T4 DNA Ligase (Fast) (5U/μL) 200 μL
10 × T4 DNA Ligase Buffer 1 mL
50% PEG 1 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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T4 DNA Ligase (Fast)
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HY-K1049
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