HEPES (1 M, Cell Culture Grade)
Based on 1 Customer Validation
MCE HEPES (1 M, Cell Culture Grade) is prepared using high-purity HEPES and sterilized by 0.22 μm membrane filtration. It can be added directly to sterile cell culture media for cell culture and other related applications.
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Storage :
4°C, 2 years. Protect from light.
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Description & Advantages
HEPES (N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)) is a zwitterionic organic buffering agent with a pKa of 7.5 at 25°C and an effective buffering range of pH 6.8–8.2. It is one of the most widely used biological buffers in life science research. HEPES exhibits several advantageous properties, including low membrane permeability, minimal interference with biochemical reactions, excellent chemical and enzymatic stability, and low absorbance in the visible and ultraviolet regions, making it well suited for use in cell culture media and a variety of other biological applications.
MCE HEPES (1 M, Cell Culture Grade) is prepared using high-purity HEPES and sterilized by 0.22 μm membrane filtration. It can be added directly to sterile cell culture media for cell culture and other related applications.
Protocol
According to the experimental requirements, add HEPES (1 M, Cell Culture Grade) to sterile cell culture medium at an appropriate ratio and mix thoroughly before use.
Note: When used as a buffering agent for cell culture media, the recommended final concentration of HEPES is 10–25 mM.
Storage
4°C, 2 years.
Protect from light.
Attention
1. This product has been sterile-filtered. Pay attention to aseptic operation to avoid contamination.
2. High concentrations of HEPES may be cytotoxic to certain cell types. Select an appropriate working concentration according to your experimental requirements.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.
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