Rapid Protein Transfer Buffer, No Ice Bath Required (10×)
MCE Rapid Protein Transfer Buffer, No Ice Bath Required (10×) is formulated with an optimized rapid protein transfer system for both wet transfer and semi-dry transfer applications. It enables the rapid and efficient transfer of proteins from PAGE gels onto PVDF or nitrocellulose (NC) membranes for Western blot analysis.
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Storage :
Room Temperature, 1 year.
- Manual (2032KB)
- COA
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
MCE Rapid Protein Transfer Buffer, No Ice Bath Required (10×) is formulated with an optimized rapid protein transfer system for both wet transfer and semi-dry transfer applications. It enables the rapid and efficient transfer of proteins from PAGE gels onto PVDF or nitrocellulose (NC) membranes for Western blot analysis.
Features of MCE Rapid Protein Transfer Buffer, No Ice Bath Required (10×)
1. High efficiency: Protein transfer can be completed in 15–40 min using the wet transfer method. Compared with conventional transfer buffers, substantially less heat is generated during transfer, eliminating the need for an ice bath.
2. Safe and convenient: Simply dilute the buffer with deionized water and absolute ethanol (or methanol) before use. No preparation of transfer buffer containing toxic reagents (e.g., formaldehyde) is required.
3. Broad compatibility: Suitable for transferring proteins from a wide range of PAGE gels, providing efficient transfer of both low- and high-molecular-weight proteins.
Protocol
1. Preparation of Transfer Buffer (1×)
Mix 100 mL of Rapid Protein Transfer Buffer, No Ice Bath Required (10×) with 800 mL of deionized water and 100 mL of absolute ethanol or methanol. Mix thoroughly before use.
Note: a. It is recommended to dilute the concentrate with deionized water first, followed by the addition of absolute ethanol or methanol.
b. The use of absolute ethanol is optional. If ethanol of a different concentration is used, adjust the volume accordingly to achieve the equivalent final ethanol concentration.
2. Protein Transfer
The transfer conditions below are provided as general guidelines and may be optimized according to the protein molecular weight, gel composition, and electrophoresis/transfer system used.
1) Standard PAGE Gels
Transfer efficiency depends on the gel concentration.
a) 1 mm Gels: Transfer at a constant current of 400 mA. Under the conditions listed in Table, proteins with molecular weights up to 150 kDa can be completely transferred. For proteins larger than 150 kDa, extend the transfer time by 5–10 min.
b) 1.5 mm Gels: Transfer at a constant current of 400 mA. Increase the transfer time by 5–10 min compared with the conditions recommended for 1 mm gels.
c) 0.75 mm Gels: Transfer at a constant current of 400 mA. Reduce the transfer time by 5–10 min compared with the conditions recommended for 1 mm gels.
2) Gradient PAGE Gels
Using a 4–15% gradient PAGE gel (1.5 mm thickness) and a 3-Color Prestained Protein Marker (10–190 kDa, HY-K1011), complete transfer of all protein bands can be achieved in 30 min at a constant current of 400 mA.
Storage
Room Temperature, 1 year.
Attention
1. Absolute ethanol is recommended for preparing the working transfer buffer.
2. If methanol is required, HPLC-grade or equivalent high-purity methanol is recommended. Analytical-grade methanol may also be used.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.
Components
| Components | HY-K1200-500 mL | HY-K1200-3 L |
|---|---|---|
| Rapid Protein Transfer Buffer, No Ice Bath Required (10×) | 500 mL | 500 mL × 6 |
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