ECM Gentle Dissociation Solution 

MCE ECM Gentle Dissociation Solution is a gentle ECM-degrading enzyme mixture derived from marine bacteria and Bacillus species, specifically formulated for efficient and low-damage digestion of in-vitro cell systems. It selectively degrades extracellular matrix components while minimizing disruption to the cell membrane and intercellular junctions, thereby significantly reducing mechanical stress during dissociation.

This product is compatible with a wide range of cell types, including stem cell colonies, primary cells, neural cells, and organoids, and is particularly well suited for gentle yet effective dissociation of brain organoids and other complex 3D structures. Compared with conventional proteases or trypsin, this mild dissociation solution provides more precise control over the digestion process, better preserves cell viability and functionality, and serves as an ideal tool for stem cell research, organoid culture, and regenerative medicine applications.

-20°C, 2 years.

Avoid repeated freeze–thaw cycles.

1. Wash the cells once with DPBS (MCE Cat. No.: HY-K3008).

2. Add 1 mL of the ECM Gentle Dissociation Solution to each well.

3. Incubate at 37°C for 7-9 min.

4. Add 500 μL FBS (MCE Cat. No.: HY-T1000) to terminate dissociation.

5. Centrifuge at 200 g for 3 min and discard the supernatant.

6. Resuspend the pellet in 500 μL DPBS, keep the cells on ice, and proceed to primary antibody incubation and subsequent steps.

1. Pre-dissociation organoids with 0.5% papain at 37°C for 15 min.

2. Add the ECM Gentle Dissociation Solution and continue dissociation for 30-45 min.

3. Transfer the mixture to a 15-mL conical tube, add 5 mL DPBS, and gently triturate to facilitate dissociation.

4. Filter the suspension through a 45-μm cell strainer.

5. Centrifuge at 300 g for 10 min, discard the supernatant.

6. Resuspend the cells in 1 mL DPBS for downstream single-cell sequencing or other applications.

1. Wash the cells once with DPBS (MCE Cat. No.: HY-K3008).

2. Add 6 mL of ECM Gentle Dissociation Solution to a 10-cm dish and digest for 3-5 min.

3. Add 3 mL FBS (half the dissociation volume) to stop the reaction.

4. Centrifuge at 300 g for 3 min and discard the supernatant.

5. Resuspend the cell pellet and passage at a 1:2-1:4 split ratio.

1. This product is sterile and should be handled using aseptic techniques. It is recommended to aliquot and store to avoid contamination.

2. This product is for R&D use only, not for drug, household, or other uses.

3. For your safety and health, please wear a lab coat and disposable gloves to operate.