AntiFade Mounting Medium
Based on 32 publication(s) in Google Scholar
MCE AntiFade Mounting Medium can slow the fluorescence quenching of various common fluorescent dyes with simple operation and good anti-fluorescence quenching effect.
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Storage :
-20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
MCE AntiFade Mounting Medium is a sealing reagent used to slow the fluorescence quenching. This product can slow the fluorescence quenching of various common fluorescent dyes with simple operation and good anti-fluorescence quenching effect.
Protocol
For adherent cell samples:
1.1 Discard as much liquid as possible after staining.
1.2 Add one drop of Antifade Mounting Medium to the slide and carefully cover the coverslip containing cells. Be careful to avoid bubbles.
1.3 Detect the fluorescence by fluorescence microscope.
For tissue section samples:
2.1 Discard as much liquid as possible after staining.
2.2 Add one drop of Antifade Mounting Medium to the tissue and carefully cover the coverslip. Be careful to avoid bubbles.
2.3 Detect the fluorescence by fluorescence microscope.
For other samples:
Other samples are processed with reference to adherent cell samples or tissue section samples.
Publications
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Journal Impact Factor
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Most Recent
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J Hazard MaterCreating a multifunctional degrader for co-mineralization of p-nitrophenol and 1,2-dichloroethane and its application in wastewater bioremediation. [Abstract]2025 May 5:488:137417. PMID: 39884036
AntiFade Mounting Medium purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 May 5:488:137417. [Abstract]
Observation of GFP fluorescence within cells of strain KTU-PDG by confocal microscope. Red fluorescence produced by cell membrane-bound FM4–64/L stain (10 μM; 37℃ for 45 min;15 μL samples were sealed with 1 μL AntiFade Mounting). Scan bar: 5 μm.
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Plant PhysiolExpansin-like A binds to pectin via electrostatic forces and remodels the plant cell wall. [Abstract]2026 Feb 6;200(2):kiag029. PMID: 41612931
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Free Radic Biol MedResveratrol induces ferroptosis in triple-negative breast cancer through NEDD4L-mediated GPX4 ubiquitination and degradation. [Abstract]2025 Aug 1:235:231-247. PMID: 40316059
AntiFade Mounting Medium purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Aug 1:235:231-247. [Abstract]
Representative result of immunofluorescence assays (the coverslips were mounted onto slides with antifade mounting medium) to detect GPX4 expression in SUM159 cells after treatment with indicated concentrations of RES for 24 h, and the quantitative data is presented.
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Emerg Microbes InfectThree-dimensional mapping of tick-borne encephalitis virus distribution in the mouse brain using a newly engineered TurboGFP reporter virus. [Abstract]2025 Dec;14(1):2542246. PMID: 40743437
AntiFade Mounting Medium purchased from MedChemExpress. Usage Cited in: Emerg Microbes Infect. 2025 Dec;14(1):2542246. [Abstract]
Cell specificity in the different tGFP-TBEV infected brain regions. Fluorescent images of mouse brain tissue sections showing various cell types within infected regions. Tissue slices were immunolabelled with anti-calbindin (red)/anti-NeuN (red)/anti-DCX antibodies/anti-GFAP antibodies/anti-Iba1 antibodies. The immunostained tissue slices were mounted with AntiFade mounting medium and stored at 4℃ until imaging.
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Commun Biol2025 Jan 9;8(1):29. PMID: 39789202
AntiFade Mounting Medium purchased from MedChemExpress. Usage Cited in: Commun Biol. 2025 Jan 9;8(1):29. [Abstract]
IF staining images showing the expression of Pax2a and Pcna in control and lima1a-/- kidneys at 7 dpi. Slides were mounted using an anti-fade mounting medium. White dashed box: Pax2 expressing RPC aggregates, and white arrows: proliferating Pax2-positive cells.
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Int ImmunopharmacolGCN2 activates macrophage autophagy to inhibit NLRP3 inflammasome-mediated acute lung injury. [Abstract]2026 Jan 1;168(Pt 1):115785. PMID: 41176906
AntiFade Mounting Medium purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2026 Jan 1;168(Pt 1):115785. [Abstract]
RAW264.7 cells were transfected with either OE-GCN2 or OE-NC and exposed to 1 μg/mL LPS for 24 h. Immunofluorescent staining of NLRP3 (green) in RAW264.7 cells. Mounted with antifade mounting medium for microscopic imaging.
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Hum CellUSF1-induced RPS6KB2 activation influences aggressive phenotype in B-cell non-Hodgkin lymphoma. [Abstract]2025 Aug 31;38(5):154. PMID: 40886264
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Acta PharmTherapeutic effect of umbilical cord mesenchymal stem cells on renal ischemia-reperfusion injury. [Abstract]2025 Apr 10;75(1):103-118. PMID: 40208785
Storage
-20°C, 1 year
Components
| Components | HY-K1042-5 mL | HY-K1042-25 mL |
|---|---|---|
| AntiFade Mounting Medium | 5 mL | 25 mL |
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