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  6. Bi-27 Serum-free Supplement (50×), Antioxidant-Free

Bi-27 Serum-free Supplement (50×), Antioxidant-Free 

Cat. No.: HY-K3014D
Manual COA Technical Support

MCE Bi-27 Serum-free Supplement (50×), Antioxidant-Free is a customized neuronal culture supplement developed by removing antioxidants components (such as vitamins, superoxide dismutase, catalase, etc.) from the standard Bi-27 formulation. It is specifically designed for investigating the roles of reactive oxygen species (ROS) in aging, cytotoxicity, apoptosis, and chronic neurological diseases. This formulation provides a stable culture environment for mechanistic studies without the interference of exogenous antioxidants.

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50 mL Ask For Quote & Lead Time

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    MedChemExpress Validation
    Immunofluorescence (IF) analysis of SD rat cortical neurons: After 7 d of in vitro culture (10× magnification), three culture conditions were compared: no Bi-27 supplement (W/O), Bi-27 Serum-free Supplement (50×), Antioxidant-Free (HY-K3014D), and Gibco B-27 Supplement, minus antioxidants (Competitor). The results showed that supplementation with B-27 significantly improved neuronal adhesion, increased cell density, and promoted more extensive and structurally developed neurite outgrowth. In contrast, the W/O group exhibited poor overall cell condition, characterized by weak attachment, reduced cell numbers, and limited neurite development. Further comparison indicated that HY-K3014D and Gibco B-27 Supplement, minus antioxidants (Competitor) performed comparably in supporting the growth and differentiation of SD rat cortical neurons, with no statistically significant differences were observed between the two groups. Overall, MCE HY-K3014D demonstrated performance equivalent to the imported competitor in maintaining neuronal morphology, promoting cell growth, and supporting neurite development.

    MedChemExpress Validation
    Analysis of CTG assay and microscopic observation on intestinal cancer cells: After 6 days of in vitro culture, three culture systems including no Bi-27 supplement (W/O), Bi-27 Serum-free Supplement (50×), Antioxidant-Free (HY-K3014D), and Gibco B-27 Supplement, minus antioxidants (Competitor) were compared. Comprehensive evaluation of cell growth status was conducted via microscopic observation (4×, 10×, 20× magnification) and CellTiter-Glo (CTG) luminescence detection. The results showed that compared with the no-Bi-27 control (W/O), supplementation with insulin-free Bi-27 improved overall cell growth, as evidenced by more intact cellular structures, clearer organoid/cell cluster morphology, and a trend toward increased size. CTG quantification further indicated no statistically significant differences (ns) in cell viability among the groups. In particular, HY-K3014D and Gibco B-27 Supplement, minus antioxidants (Competitor) exhibited comparable performance in supporting cell viability, with neither showing a significant difference relative to the control. In summary, within this colorectal cancer cell culture system, HY-K3014D Bi-27 demonstrated performance equivalent to the imported competitor in supporting cell growth and maintaining cell viability, with no significant differences observed.

    • Description

    • Storage

    • Protocol

    • Attention

    • Components

    • Documentation

    Description
    & Advantages

    MCE Bi-27 Serum-free Supplement (50×), Antioxidant-Free is a customized neuronal culture supplement developed by removing antioxidants components (such as vitamins, superoxide dismutase, catalase, etc.) from the standard Bi-27 formulation. It is specifically designed for investigating the roles of reactive oxygen species (ROS) in aging, cytotoxicity, apoptosis, and chronic neurological diseases. This formulation provides a stable culture environment for mechanistic studies without the interference of exogenous antioxidants.

    The recommended working concentration of Bi-27 Supplement is 2% (v/v), corresponding to a 1:50 dilution before use in the culture medium.
    Storage

    -20°C, 2 years

    Keep away from light

    Shipping with dry ice
    Protocol

    Prepare complete Neuronal basal Medium

    1. Thaw MCE Bi-27 Serum-free Supplement (50×), Antioxidant -Free overnight at 4°C.

    2. Aseptically add 2% Bi-27 and 0.5mM L-Glutamine to the Neuronal Basal Medium before use.

    3. Primary Hippocampal Neuron Culture: Add 25 µM of L-glutamate to the complete Neuronal Basal Medium. The medium can be replaced after 4 days of culture, and glutamate should not be added to the medium after this change.

    Note: Once prepared, the complete Neuronal Basal Medium can be stored at 4°C for two weeks, protected from light.
    Attention

    1. This product appears as a light yellow liquid. The color may not be consistent following freezing, but it can fully dissolve in a 37°C water bath.

    2. It is recommended to appropriately aliquot the solution to avoid repeat freeze-thaw cycles.

    3. Pay attention to aseptic operation to avoid contamination.

    4. This product is for R&D use only, not for drug, household, or other uses.

    5. For your safety and health, please wear a lab coat and disposable gloves to operate.
    Components
    Components HY-K3014C-10 mL HY-K3014C-50 mL
    Bi-27 Serum-free Supplement (50×), Antioxidant-Free 10 mL 10 mL × 5
    Documentation

    Manual (1297 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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