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  5. Lentivirus
  6. Lentivirus Concentration Solution (5×)

Lentivirus Concentration Solution (5×) 

Cat. No.: HY-K2031
Manual COA SDS Technical Support

MCE Lentivirus Concentration Solution (5×) is a high-efficiency polymer solution optimized for lentiviral particle enrichment, providing a simple, rapid, and effective method for lentivirus concentration.

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100 mL In-stock
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    MedChemExpress Validation
    Viral supernatant was concentrated 10-fold using Lentivirus Concentration Solution (5×) (HY-K2031). Different volumes (20, 10, 5, and 2 μL) 10× concentrated virus and unconcentrated lentivirus were used to infect approximately 5 × 104 HEK293T cells. At 48 h post-infection, EGFP (green fluorescence) and mCherry (red fluorescence) expressions were assessed. As little as 2 μL of concentrated virus achieved fluorescence signals comparable to 20 μL of unconcentrated virus. The 10-fold concentration increased infection efficiency by more than one order of magnitude, substantially reducing viral usage and lowering the risk of cytotoxicity to target cells.

    • Description

    • Storage

    • Application

    • Attention

    • Documentation

    Description
    & Advantages

    The lentiviral vector is a gene therapy vehicle derived from HIV-1, capable of stably integrating exogenous genes into the host cell genome. It features a broad host range, enables efficient infection of both dividing and non-dividing cells, and ensures long-term and stable expression of transgenes. Therefore, it has become a powerful tool for gene delivery, widely used in gene overexpression, RNA interference, microRNA studies, and in vivo animal experiments across various cell lines.

    MCE Lentivirus Concentration Solution (5×) is a high-efficiency polymer solution optimized for lentiviral particle enrichment, providing a simple, rapid, and effective method for lentivirus concentration. To use, simply mix the lentiviral supernatant with the reagent at the recommended ratio, incubate briefly, and centrifuge using a standard laboratory centrifuge to obtain a visible lentiviral pellet.

     

    Features of MCE Lentivirus Concentration Solution (5×)

    1. Simple, rapid, and efficient: No ultracentrifugation required; the entire process can be completed within 4 h.

    2. Excellent concentration performance: Increases viral titer by more than 10-100-fold.

    3. High recovery and preserved activity: Achieves up to 90% viral recovery while maintaining viral infectivity and biological activity during the concentration process.

    4. Broad applicability: Compatible with all types of lentiviral particles for downstream applications.
    Storage

    -20°C, 1 year
    Application
    Attention

    1. All lentivirus-related proceduresmust be conducted in a BSL-2 (biosafety level 2) biosafety cabinet to ensure safe handling and containment.

    2. This product is for R&D use only, not for drug, household, or other uses.

    3. For your safety and health, please wear a lab coat and disposable gloves to operate.
    Documentation

    Manual (2336 KB) SDS (251 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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