TBS Powder (1 L of 1×) 

TBS buffer is widely used as washing buffer in immunocytochemistry, FISH or ELISA, and can also be used for antibody dilution and preparation of blocking solution.

MCE TBS Powder (1 L of 1×) consists of NaCl, KCl and Tris. Each pouch can be diluted to 1 L 1× TBS buffer, in which the concentration of NaCl is 137 mM, the concentration of KCl is 2.7 mM and the concentration of Tris is 25 mM. This product provides a convenient way to make TBS buffer and eliminates the need to weigh and mix individual components.

Store at room temperature for 3 years.

1. Add 1 pouch of TBS Powder into the cleaned beaker, dissolve with 600 mL distilled water under a magnetic stirrer.

2. Add distilled water to the solution in step 1 until the total volume is 1 L. The final solution is 1× TBS buffer.

Note: The pH of the 1× TBS buffer is 7.4 ± 0.15 @ 25°C.