1. Recombinant Proteins
  2. Recombinant Protein Expression Service

Recombinant Protein Expression Service

Recombinant protein is a protein encoded by a recombinant DNA that has been cloned in a system that supports the expression of the gene and translation of mRNA. The host cells used for recombinant protein production can be derived from bacteria, mammalian cells, insects and yeast. Recombinant proteins have been widely used in most hot research areas such as immune checkpoints, antibody-drug targets, CAR-T cell therapy targets, Fc receptors, influenza viral proteins and cytokines.

MedChemExpress (MCE) has professional protein expression and purification technology with customized services of high-purity active protein from mg to g scale. MCE multiple protein expression systems include bacteria, yeast, insect and mammalian expression systems. MCE focuses on high-quality protein production and seeks to partner with scientists all over the world to accelerate scientific discovery.

Advantages

  • One-stop service from gene design to protein preparation
  • Independent research and development of a variety of
    high expression vectors and strains
  • Professional R & D team of recombinant protein technology
  • Providing customized services of high-purity protein from
    mg to g
  • Large-scale protein production platform

Service Process

MCE provides a one-stop service from genes to proteins. Customers can flexibly choose services or personalized services according to their own needs.

Service Process

Codon Optimization Gene Synthesis Construction of
Expression Vector
Strain Screening Protein Expression Protein Purification Protein Delivery
  • The specific process of recombinant protein expression service

Service Types

MCE has five technical service platforms: E.coli expression system, yeast expression system, mammalian expression system, insect expression system and inclusion body protein renaturation.

Project Content Cycle Deliverable
E.coli Expression System Construction of Expression Vector 1-2 weeks Expression Plasmid /
Construction Report
Protein Expression 1 week Expression Report
Flask Fermentation and Protein Purification 3 weeks All Qualified Proteins /
Purification Report
Inclusion Body Protein Renaturation Construction of Expression Vector 1-2 weeks Sequencing Report
Protein Expression 1 week Expression Report
Small-scale Protein Renaturation 2-3 weeks Refolding Protein
Mass Protein Production 2-4 weeks mg-g Level Refolding Protein
Yeast Expression System Construction of Expression Vector 1-2 weeks Construction Report
Electrotransformation 2 weeks 5 Positive Strains
Screening of High Copy Strains 1 week 1 High Copy Strain
Protein Expression 3 weeks Expression Report
Protein Purification 3 weeks Protein/ Purification Report
Mammalian Expression System Construction of Expression Vector 1-2 weeks Construction Report
HEK293/CHO Cell Transfection and Expression Detection 3 weeks Expression Report
1L HEK293/CHO Cell Transfection and Protein Purification 3 weeks All Qualified Proteins /
Purification Report
Insect Expression System Construction of Expression Vector 2 weeks Construction Report
Preparation of Bacmid 1 week Preparation Report
Cell Transfection and Expression Detection 4-5 weeks Expression Report
Protein Purification 4 weeks Protein / Purification Report