Copy Link Print Information

Basement Membrane Matrix (Phenol Red)

21 Cited Publications
Customer Review

Based on 21 publication(s) in Google Scholar

MCE Basement Membrane Matrix (Phenol Red) is primarily composed of a natural basement membrane matrix derived from mouse tumors. This product is mainly for studies of tumor invasion, angiogenesis, and organoid culture.

  • Storage :
    -20°C, 2 years. Avoid repeated freezing and thawing. Shipping with dry ice.
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
  • Basement Membrane Matrix (Phenol Red) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Description & Advantages

MCE Basement Membrane Matrix (Phenol Red) is a natural basement membrane matrix extracted from mouse tumors and is composed mainly of various growth factors and extracellular matrix components. The main extracellular matrix components are: Laminin, Col-IV, Entactin, Heparan sulphate proteoglycans, etc. The main growth factor components are: insulin-like growth factor (IGF-1), transforming growth factor beta (TGF-β), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), fibroblast growth factor (bFGF), etc.

MCE Basement Membrane Matrix (Phenol Red) is suitable for studies of tumor invasion, angiogenesis, and organoid culture.

Protocol

Recommended Application Methods

MCE Standard Basement Membrane Matrix (Phenol Red) can be used in the following four application methods.

Application Method Primary Function Common Applications
Thin Gel Forms an approximately 0.5 mm gel layer Primary cell culture, cell migration and invasion assays
Thin Coating Does not form a gel; forms an ECM coating layer only Primary cell culture, iPSC culture, and cell adhesion assays
Thick Gel Forms a 1-2 mm three-dimensional gel structure Angiogenesis assays and aortic ring assays
Gel Embedding Cells are completely embedded within the matrix Organoid culture and tumor spheroid invasion assays

Preparation Before Experiment

• Slight variations in protein concentration may occur between different batches. Please refer to the corresponding Certificate of Analysis (COA) for specific information.

• To achieve optimal experimental reproducibility, it is recommended to calculate the required volume based on the actual protein concentration and dilute the matrix using pre-chilled serum-free medium.

• Maintain low temperature conditions (ice bath or 4°C) throughout the entire procedure to prevent premature gelation of the basement membrane matrix.

Storage

-20°C, 2 years.

Avoid repeated freezing and thawing.

Shipping with dry ice.

Components

Components HY-K6001-1 mL HY-K6001-5 mL HY-K6001-10 mL
Basement Membrane Matrix (Phenol Red) 1 mL 5 mL 10 mL

Documentation

Keywords

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)

    Basement Membrane Matrix (Phenol Red) purchased from MedChemExpress. Usage Cited in: ACS Omega. 2024 Jan 16;9(4):4974-4985. [Abstract]

    Angiogenesis images after HUs culturing with different Cu2O nanoparticle concentrations.

  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)

    Basement Membrane Matrix (Phenol Red) purchased from MedChemExpress. Usage Cited in: ACS Omega. 2024 Jan 16;9(4):4974-4985. [Abstract]

    Transwell results after HUs culturing with different Cu2O nanoparticle concentrations.

  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)

    Basement Membrane Matrix (Phenol Red) purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 May 1;10(9):e30603. [Abstract]

    After overexpression of RBMS3 and activation of the Wnt/β-catenin signalling pathway, the migratory activity of ID8 cells was detected by transwell.

  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)

    Basement Membrane Matrix (Phenol Red) purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 May 1;10(9):e30603. [Abstract]

    After overexpression of RBMS3 and activation of the Wnt/β-catenin signalling pathway, the invasive activity of ID8 cells was detected by transwell.

Assay Types
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
    1/16

    MedChemExpress Validation
    Cell type: HUVECs (immortalized cell line, mixed with primary cells at passages P10-P18)
    Seeding density: 8,000 cells/well (1.6 × 105 cells/mL)
    Matrix gel: 8 μL
    Complete culture medium: 50 μL
    Under identical tube formation assay conditions, MCE matrix gel demonstrated superior tube-forming performance compared with competitor brands at both undiluted and 2:1 dilution conditions. The tubular structures formed with MCE Matrigel (Basement Membrane Matrix (Phenol Red), HY-K6001) were more complete and continuous, with clearer and more stable network architecture.

  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
    1/16

    MedChemExpress Validation
    Cell type: HUVECs (immortalized cell line, mixed with primary cells at passages P10-P18)
    Seeding density: 8,000 cells/well (1.6 × 105 cells/mL)
    Matrix gel: 8 μL
    Complete culture medium: 50 μL
    Under identical tube formation assay conditions, the tube-forming performance of MCE matrix gel and competitor brands was compared at both undiluted and 2:1 dilution conditions. The tube-forming capacity of different production batches of MCE matrix gel (Basement Membrane Matrix (Phenol Red), HY-K6001) was consistent, with complete and continuous tubular structures and clear, stable network architecture. The overall tube formation performance was stable and reproducible, with minimal batch-to-batch variation, demonstrating good batch consistency.

  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
    1/16

    MedChemExpress Validation
    Under identical tube formation assay conditions, the tube-forming performance of MCE matrix gel (Basement Membrane Matrix (Phenol Red), HY-K6001) and competitor brands was compared at both undiluted and 2:1 dilution conditions. The tube-forming capacity of different production batches of MCE matrix gel was consistent, with complete and continuous tubular structures and clear, stable network architecture. The overall tube formation performance was stable and reproducible, with minimal batch-to-batch variation, demonstrating good batch consistency.

  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
    1/16

    MedChemExpress Validation
    MCE Basement Membrane Matrix (Phenol Red) (HY-K6001) supported cell invasion at a level comparable to that of the competitor matrix.

  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
    1/16

    Basement Membrane Matrix (Phenol Red) purchased from MedChemExpress. Usage Cited in: ACS Omega. 2024 Jan 16;9(4):4974-4985. [Abstract]

    Angiogenesis images after HUs culturing with different Cu2O nanoparticle concentrations.

  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
    1/16

    Basement Membrane Matrix (Phenol Red) purchased from MedChemExpress. Usage Cited in: ACS Omega. 2024 Jan 16;9(4):4974-4985. [Abstract]

    Transwell results after HUs culturing with different Cu2O nanoparticle concentrations.

  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
    1/16

    Basement Membrane Matrix (Phenol Red) purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 May 1;10(9):e30603. [Abstract]

    After overexpression of RBMS3 and activation of the Wnt/β-catenin signalling pathway, the migratory activity of ID8 cells was detected by transwell.

  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
    1/16

    Basement Membrane Matrix (Phenol Red) purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 May 1;10(9):e30603. [Abstract]

    After overexpression of RBMS3 and activation of the Wnt/β-catenin signalling pathway, the invasive activity of ID8 cells was detected by transwell.

  • Basement Membrane Matrix (Phenol Red) Product Image
    1/16
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
  • Experimental Validation Results for Basement Membrane Matrix (Phenol Red)
  • Basement Membrane Matrix (Phenol Red) Product Image