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  5. Basement Membrane Matrix
  6. Basement Membrane Matrix (Phenol Red)

Basement Membrane Matrix (Phenol Red) 

Cat. No.: HY-K6001
Manual COA SDS Technical Support
Organoid Culture

MCE Basement Membrane Matrix (Phenol Red) is primarily composed of a natural basement membrane matrix derived from mouse tumors. This product is mainly for studies of tumor invasion, angiogenesis, and organoid culture.

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    Basement Membrane Matrix (Phenol Red) purchased from MCE. Usage Cited in: ACS Omega. 2024 Jan 16;9(4):4974-4985.

    Transwell results after HUs culturing with different Cu2O nanoparticle concentrations.

    Basement Membrane Matrix (Phenol Red) purchased from MCE. Usage Cited in: ACS Omega. 2024 Jan 16;9(4):4974-4985.

    Angiogenesis images after HUs culturing with different Cu2O nanoparticle concentrations.

    Basement Membrane Matrix (Phenol Red) purchased from MCE. Usage Cited in: Heliyon. 2024 May 1;10(9):e30603.  [Abstract]

    After overexpression of RBMS3 and activation of the Wnt/β-catenin signalling pathway, the migratory activity of ID8 cells was detected by transwell.

    Basement Membrane Matrix (Phenol Red) purchased from MCE. Usage Cited in: Heliyon. 2024 May 1;10(9):e30603.  [Abstract]

    After overexpression of RBMS3 and activation of the Wnt/β-catenin signalling pathway, the invasive activity of ID8 cells was detected by transwell.

      MedChemExpress Validation
      Cell type: HUVECs (immortalized cell line, mixed with primary cells at passages P10-P18)
      Seeding density: 8,000 cells/well (1.6 × 105 cells/mL)
      Matrix gel: 8 μL
      Complete culture medium: 50 μL
      Under identical tube formation assay conditions, MCE matrix gel (Basement Membrane Matrix (Phenol Red), HY-K6001) demonstrated superior tube-forming performance compared with competitor brands at both undiluted and 2:1 dilution conditions. The tubular structures formed with MCE matrix gel were more complete and continuous, with clearer and more stable network architecture.

      MedChemExpress Validation
      Cell type: HUVECs (immortalized cell line, mixed with primary cells at passages P10-P18)
      Seeding density: 8,000 cells/well (1.6 × 105 cells/mL)
      Matrix gel: 8 μL
      Complete culture medium: 50 μL
      Under identical tube formation assay conditions, the tube formation effects of different batches of MCE Matrigel (Basement Membrane Matrix (Phenol Red), HY-K6001) were compared under both undiluted and 2:1 diluted conditions.Different batches of MCE Matrigel exhibited consistent tube formation ability. The formed tubular structures were intact and continuous, with clear and stable network structures.The overall tube formation effect was stable and reproducible, with minor differences between batches, indicating good batch-to-batch stability.

      MedChemExpress Validation
      Cell type: HUVECs (immortalized cell line, mixed with primary cells at passages P10-P18)
      Seeding density: 8,000 cells/well (1.6 × 105 cells/mL)
      Matrix gel: 8 μL
      Complete culture medium: 50 μL
      Under identical tube formation assay conditions, the tube formation effects of MCE Matrigel (Basement Membrane Matrix (Phenol Red), HY-K6001) and competitor brand Matrigel were compared under both undiluted and 2:1 diluted conditions. MCE Matrigel exhibited superior tube formation ability, with more intact and continuous tubular structures and clearer, more stable network structures.

      • Description

      • Storage

      • Protocol

      • Components

      • Documentation

      Description
      & Advantages

      MCE Basement Membrane Matrix (Phenol Red) is a natural basement membrane matrix extracted from mouse tumors and is composed mainly of various growth factors and extracellular matrix components. The main extracellular matrix components are: Laminin, Col-IV, Entactin, Heparan sulphate proteoglycans, etc. The main growth factor components are: insulin-like growth factor (IGF-1), transforming growth factor beta (TGF-β), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), fibroblast growth factor (bFGF), etc.

      MCE Basement Membrane Matrix (Phenol Red) is suitable for studies of tumor invasion, angiogenesis, and organoid culture.
      Storage

      -20°C, 2 years.

      Avoid repeated freezing and thawing.

      Shipping with dry ice
      Protocol

      a. Use a pre-cooled pipette to blow the matrix gel evenly after thawing.

      b. Use pre-cooled serum-free medium to dilute according to experimental requirements. Refer to the Experimental Conditions table for final concentrations.

      c. Place the culture plate on ice and inject the substrate gel dilution slowly against the wall to avoid air bubbles. Refer to the Experimental Conditions table for the volume injected.

      d. Transfer the culture plate to 37℃ incubator gently. After gelling, aspirate off excess supernatant. Refer to the Experimental Conditions table for gel time.

       

      Note: When thawing the product for the first time, immerse the vial in crushed ice and place it in a 4°C to melt. After melting, mix thoroughly before aliquoting, and then store at -20°C.
      Components
      Components HY-K6001-5 mL HY-K6001-10 mL
      Basement Membrane Matrix (Phenol Red) 5 mL 10 mL
      Documentation

      Manual (108 KB) SDS (105 KB)
      Help & FAQs
      • Do most proteins show cross-species activity?

        Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

      Keywords:

      Matrix-Gel，Matrigel，Extracellular matrix，scaffolds

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