TBE Powder (1 L of 1×)
Based on 1 Customer Validation
MCE TBE Powder (1 L of 1×) consists of Tris, Boric Acid and EDTA, and is widely used in agarose gel electrophoresis of nucleic acid. The 10 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.
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Storage :
Store at room temperature for 3 years.
Description & Advantages
TBE buffer is widely used in biological experiments, especially in agarose gel electrophoresis of nucleic acid. DNA fragments (< 1 kb) can be separated effectively under TBE buffer. TBE buffer is also suitable for long-time electrophoresis.
MCE TBE Powder (1 L of 1×) consists of Tris, Boric Acid and EDTA. Each pouch can be diluted to 1 L 1× TBE buffer, in which the concentration of Tris is 89 mM, the concentration of Boric Acid is 89 mM and the concentration of EDTA is 2 mM. This product provides a convenient way to make TBE solution and eliminates the need to weigh and mix individual components.
Protocols
1. Add 1 pouch of TBE Powder into the cleaned beaker, dissolve with 600 mL distilled water under a magnetic stirrer.
2. Add distilled water to the solution in step 1 until the total volume is 1 L. The final solution is 1× TBE buffer.
Note:
a. The pH of the 1× TBE buffer is 8.35 ± 0.15 @ 25°C.
b. The usual working concentration of TBE buffer is 0.5×, please dilute before use.
Storage
Store at room temperature for 3 years.
Components
| Components | HY-K1016-10 pouches | HY-K1016-50 pouches |
|---|---|---|
| TBE Powder (1 L of 1×) | 10 pouches | 5 × 10 pouches |