TBE Powder (1 L of 1×) 

TBE buffer is widely used in biological experiments, especially in agarose gel electrophoresis of nucleic acid. DNA fragments (< 1 kb) can be separated effectively under TBE buffer. TBE buffer is also suitable for long-time electrophoresis.

MCE TBE Powder (1 L of 1×) consists of Tris, Boric Acid and EDTA. Each pouch can be diluted to 1 L 1× TBE buffer, in which the concentration of Tris is 89 mM, the concentration of Boric Acid is 89 mM and the concentration of EDTA is 2 mM. This product provides a convenient way to make TBE solution and eliminates the need to weigh and mix individual components.

Store at room temperature for 3 years.

1.   Add 1 pouch of TBE Powder into the cleaned beaker, dissolve with 600 mL distilled water under a magnetic stirrer.

2.   Add distilled water to the solution in step 1 until the total volume is 1 L. The final solution is 1× TBE buffer.

Note:

a.   The pH of the 1× TBE buffer is 8.35 ± 0.15 @ 25°C.

b.   The usual working concentration of TBE buffer is 0.5×, please dilute before use.