Cardiomyocyte Dissociation Solution
Based on 1 Customer Validation
MCE Cardiomyocyte Dissociation Solution is specifically designed for the dissociation of cardiomyocytes following PSC differentiation and selection. It contains highly active collagenase, dispase, and neutral protease, which work synergistically with a low concentration of recombinant serine protease to efficiently and gently dissociate densely adherent cardiomyocytes into a single-cell suspension.
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Storage :
-20°C, 2 years. Avoid repeated freeze/thaw cycles. Shipping with dry ice.
- Manual (1587KB)
- COA
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Description & Advantages
MCE Cardiomyocyte Dissociation Solution is specifically designed for the dissociation of cardiomyocytes following PSC differentiation and selection. It contains highly active collagenase, dispase, and neutral protease, which work synergistically with a low concentration of recombinant serine protease to efficiently and gently dissociate densely adherent cardiomyocytes into a single-cell suspension. Following dissociation, digestion can be directly terminated using Cardiomyocytes (iCMs) Maturation Medium without the need to add a protease inhibitor. The resulting cardiomyocytes maintain high viability and functional activity, while preserving membrane potential integrity and physiological rhythmicity to the greatest extent possible.
This product is recommended for use in conjunction with the Human iPSC/ESC Cardiomyocyte Induction and Differentiation Kit (Cat. No. HY-K6303).
Protocol
For detailed procedures, please refer to the Replating and Confocal Imaging Identification section of the Human iPSC/ESC Cardiomyocyte Induction Differentiation Kit (Cat. No.: HY-K6303)– iCMs protocol.
1. Reagent Preparation: Thaw the cardiomyocyte dissociation solution overnight in a refrigerator at 4 °C prior to use.
Note: After the initial thawing, it is recommended to aliquot the solution into appropriate volumes for storage to avoid repeated freeze-thaw cycles.
2. Cell Dissociation: Remove the original culture medium and wash the cells three times with DPBS. Add 500 μL of cardiomyocyte dissociation solution to each well and incubate at 37°C for 12–15 min, until the intercellular gaps enlarge and the cell bodies retract.
3. Termination of Dissociation: Centrifuge at 150× g for 3 min and discard the dissociation solution. Gently resuspend the cells in cardiomyocyte progenitor differentiation medium supplemented with CEPT Cocktail Plus.
4. Cell Seeding and Confocal Imaging Identification.
Storage
-20°C, 2 years. Avoid repeated freeze/thaw cycles.
Shipping with dry ice.
Attention
1. This product is sterile and should be handled using aseptic techniques. It is recommended to aliquot and store to avoid contamination.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.
Components
| Components | HY-K6023-100 mL | Storage |
|---|---|---|
| Cardiomyocyte Dissociation Solution | 100 mL | -20°C, 2 years. |
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