Basement Membrane Matrix GFR 

•   MCE Basement Membrane Matrix GFR is a natural basement membrane matrix extracted from mouse tumors and is composed mainly of various growth factors and extracellular matrix components. The main extracellular matrix components are: Laminin, Col-IV, Entactin, Heparan sulphate proteoglycans, etc. The main growth factor components are: insulin-like growth factor (IGF-1), transforming growth factor beta (TGF-β), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), fibroblast growth factor (bFGF), etc. The percentage of each of these growth factors is lower than that of standard basement membrane matrix.

•   MCE Basement Membrane Matrix GFR (Phenol Red) reduces the background signal induced by growth factors and is suitable for organoid culture. It is also for research applications with high requirements for basement membrane preparation and has no effect on subsequent experiments requiring color detection.

-20°C, 2 years.

Avoid repeated freezing and thawing.

a. Use a pre-cooled pipette to blow the matrix gel evenly after thawing.

b. Use pre-cooled serum-free medium to dilute according to experimental requirements. Refer to the Experimental Conditions table for final concentrations.

c. Place the culture plate on ice and inject the substrate gel dilution slowly against the wall to avoid air bubbles. Refer to the Experimental Conditions table for the volume injected.

d. Transfer the culture plate to 37℃ incubator gently. After gelling, aspirate off excess supernatant. Refer to the Experimental Conditions table for gel time.