Animal Tissue Direct PCR Kit (with Dye) 

MCE Animal Tissue Direct PCR Kit (with Dye) is specifically designed for direct amplification from a variety of animal tissue samples. It enables rapid release of genomic DNA from animal tissues (such as insect legs/wings, mouse tails, ears, toes, skin, and internal organs) in a single step, allowing the lysate to be used directly for downstream PCR amplification without the need for conventional DNA extraction and purification procedures, thereby significantly simplifying the workflow.

The kit is supplied with a ready-to-use PCR master mix containing all components required for amplification, including Taq DNA polymerase, dNTPs, optimized buffer system, and tracking dyes. Only primers and template need to be added to initiate the PCR reaction. The amplified products can be directly analyzed by agarose gel electrophoresis without the addition of loading buffer, effectively reducing operational complexity and the risk of contamination. This kit is suitable for applications such as animal genotyping and transgenic identification.

Features of MCE Animal Tissue Direct PCR Kit (with Dye):

1. Fast and Convenient: Tissue samples do not require cutting or grinding. Genomic DNA can be prepared in a single step, with template preparation completed in as little as 10 min and PCR amplification in approximately 50 min.

2. Low Sample Requirement: Only a small amount of tissue is required for effective lysis.

3. High Specificity and Efficiency: Optimized Taq polymerase system with strong template affinity and high amplification specificity, ideal for genotyping and transgenic analysis.

4. User-Friendly Operation: The master mix contains tracking dyes, allowing PCR products to be directly loaded for electrophoresis.

5. High-Throughput Compatibility: Both lysis and amplification steps can be performed in a 96-well plate format, making it suitable for high-throughput screening.

Lysis Buffer: 4°C, 1 year. If not be used for an extended period, it is recommended to aliquot it for storage.

Proteases, Direct PCR Master Mix (2×, with Dye), PCR Enhancer (5×): -20°C, 1 year. Avoid repeated freeze-thaw cycles.

1. To prevent cross-contamination between samples, sampling tools should be thoroughly cleaned after each use. It is recommended to immerse the cutting edge or any part in direct contact with the sample in 2% sodium hypochlorite solution, rinse repeatedly, and then wipe dry with clean tissue before reuse. For improved efficiency, multiple sets of sampling tools may be prepared and cleaned collectively to ensure that each sample is handled with contamination-free instruments.

2. Freshly collected animal tissues are recommended for optimal results. For long-term frozen samples, repeated freeze–thaw cycles should be avoided, as they may lead to genomic DNA degradation and consequently affect PCR amplification efficiency and data reliability.

3. This product is for R&D use only, not for drug, household, or other uses.

4. For your safety and health, please wear a lab coat and disposable gloves to operate.