15 kb DNA Marker
Based on 1 Customer Validation
The 15 kb DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis.The 250 μL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
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Storage :
-20°C, 2 years.
Avoid repetitive freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
The 15 kb DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis. The Marker contains 8 double-stranded DNA fragments ranging from 500 bp to 15 kb. 5 μL of this product contains about 80 ng for the 4000 bp band, and about 30 ng for the other bands.
Protocol
General Protocol
1. This product is supplied in 1× DNA Loading Buffer and can be loaded directly for agarose gel electrophoresis without adding loading buffer.
2. Completely thaw 15 kb DNA Marker, mix gently, and briefly centrifuge to collect the solution at the bottom of the tube.
3. Load 5 μL Marker per lane as a recommended starting amount. Select an appropriate agarose concentration according to the DNA fragment size of interest and perform electrophoresis under standard conditions.
4. The Marker contains 8 double-stranded DNA fragments ranging from 500 bp-15 kb. In each 5 μL, the 4000 bp contain approximately 80 ng DNA, while the other bands contain approximately 30 ng DNA, providing prominent reference bands for size estimation.
5. After electrophoresis, image the gel according to the nucleic acid stain used and compare the migration of the sample bands with the Marker.
Note: Store at -20°C and avoid repeated freeze-thaw cycles. Aliquoting is recommended for frequent use.
Storage
-20°C, 2 years.
Avoid repetitive freeze-thaw cycles.
Components
| Components | HY-K0808-250 μL | HY-K0808-500μL | HY-K0808-1 mL |
|---|---|---|---|
| 15 kb DNA Marker | 250 μL | 250 μL × 2 | 250 μL × 4 |
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