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  6. ATP Assay Kit

ATP Assay Kit 

Cat. No.: HY-K0314
Manual COA SDS Technical Support

MCE ATP Assay Kit provides a rapid method to measure intracellular ATP. The kit is suitable for ATP determination in cells, tissue, and other biological samples.

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100 T In-stock
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    ATP Assay Kit purchased from MCE. Usage Cited in: Adv Mater. 2025 Apr;37(13):e2419149.  [Abstract]

    ATP content in HaCaT cells.

    ATP Assay Kit purchased from MCE. Usage Cited in: J Hazard Mater. 2025 Jun 27:495:139088.  [Abstract]

    Changes in ATP levels in cells were detected using the luciferase method after 24 h of MK2206 and 6PPDQ treatments on A549 and H1299 cells.

    ATP Assay Kit purchased from MCE. Usage Cited in: Nano Today. 2025 Jun.

    Results of extracellular ATP released by 4T1 cells incubated with different formulations for 8 h.

    ATP Assay Kit purchased from MCE. Usage Cited in: Mater Today Bio. 2025 Nov 13:35:102547.  [Abstract]

    The ATP levels in the T24 cells after treated by Gox, Gox-Mn NPs, Cys-Gox-Mn NPs, and M@Cys-Gox-Mn NPs, respectively. Gox-equivalent dose: 0.2 μg/ml, 2 h incubation.

    ATP Assay Kit purchased from MCE. Usage Cited in: Innov Food Sci Emerg Technol. 2025 Nov 21;107:104375.

    ATP level of A. niger during heat and RF treatment.

    • Description

    • Storage

    • Components

    • Documentation

    Description
    & Advantages

    MCE ATP Assay Kit provides a rapid method to measure intracellular ATP. The principle is that ATP reacts with the substrate luciferin under the action of Firefly Luciferase to produce fluorescence. When both Luciferase and luciferin are in excess, the fluorescence intensity is proportional to the ATP concentration within a certain range. By measuring the fluorescence intensity, the ATP concentration in the sample can be determined.

    The kit is suitable for ATP determination in cells, tissue, and other biological samples. A single package of this kit (100T) is sufficient to test at least 100 samples.
    Storage

    -20℃, 6 months; -80℃, 1 year

    Keep away from light and avoid repeat freeze-thaw cycles
    Components
    Components HY-K0314-100 T
    ATP Detection Reagent A (100×) 100 μL
    ATP Detection Reagent B (10×) 1 mL
    ATP Detection Reagent C (10×) 3 mL
    ATP Detection Reagent D (100×) 100 μL
    ATP Standard Solution (0.5 mM) 250 μL
    ATP Detection Solution (20×) 1.8 mL
    Documentation

    Manual (3487 KB) SDS (251 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Extracellular ATP AssayATP Content Detection kitATP Detection

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