1. Dye Reagents
  2. JC-1

JC-1 (Synonyms: CBIC2)

Cat. No.: HY-15534 Purity: ≥99.0%
Handling Instructions

JC-1 (CBIC2) is a fluorescent lipophilic carbocyanine dye used to measure mitochondrial membrane potential. JC-1 forms complexes known as J-aggregates at high ΔΨm. Aggregates of JC-1 emit an orange-red fluorescence (Ex/Em=585/590 nm). While in cells with low ΔΨm, JC-1 remains in the monomeric form. JC-1 monomers emit a green fluorescence (Ex/Em=510/527 nm).

For research use only. We do not sell to patients.

JC-1 Chemical Structure

JC-1 Chemical Structure

CAS No. : 3520-43-2

Size Price Stock Quantity
2 mg USD 106 In-stock
Estimated Time of Arrival: December 31
5 mg USD 198 In-stock
Estimated Time of Arrival: December 31
10 mg USD 330 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1408 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 57 publication(s) in Google Scholar

Top Publications Citing Use of Products

55 Publications Citing Use of MCE JC-1

    JC-1 purchased from MCE. Usage Cited in: New J Chem. 2017 41(23).

    Measurement of mitochondria membrane potential by JC-1 staining. HeLa cells are incubated with or without WOs for 24 h, followed by NIR irradiation for NIR and WOs + NIR groups.

    JC-1 purchased from MCE. Usage Cited in: Mol Cancer. 2019 Apr 10;18(1):85.

    KRA-533 induces caspase 3 activation and reduces mitochondrial membrane potential in NSCLC cells. The measurement of mitochondrial membrane potential by JC-1 staining.
    • Biological Activity

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    Description

    JC-1 (CBIC2) is a fluorescent lipophilic carbocyanine dye used to measure mitochondrial membrane potential. JC-1 forms complexes known as J-aggregates at high ΔΨm. Aggregates of JC-1 emit an orange-red fluorescence (Ex/Em=585/590 nm). While in cells with low ΔΨm, JC-1 remains in the monomeric form. JC-1 monomers emit a green fluorescence (Ex/Em=510/527 nm).

    In Vitro

    Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
    Labeling of Cells:
    1. Culture cells in 6-, 12- , 24-, or 96-well plates at a density of 5× 105 cells/mL. Incubate the cells according to your normal protocol.
    2. Ensure that the JC-1 and DMSO has equilibrated to room temperature, and then prepare a 200 μM stock solution by dissolving the contents of one vial in DMSO provided.
    3. For the control tube, allow the vial of CCCP has come to room temperature, add 1 μL of CCCP (50 mM). Incubate cells at 37°C for 5 minutes.
    4. Add 10 μL JC-1 (200 μM) per well to make the final concentration at 2 μM. Incubate cells at 37°C, 5% CO2, for 15-20 minutes. If additional labeling followed, for example with an annexin V, begin with step 2.a. If not, proceed with step 1.e.
    5. After incubation, centrifuge cells for 3-4 minutes at 400× g at 4°C, carefully aspirate the supernant.
    6. Wash cells twice with PBS (1×): add 2 mL PBS (1×) to suspend cells and vortex to mix thoroughly. Centrifuge cells for 3-4 minutes at 400× g at 4°C, carefully aspirate the supernant.
    7. Add 500 μL PBS (1×) to suspend cells. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    652.23

    Formula

    C25H27Cl4IN4

    CAS No.
    SMILES

    ClC1=C(Cl)C=C([N+](CC)=C(/C=C/C=C2N(C(C=C(Cl)C(Cl)=C3)=C3N\2CC)CC)N4CC)C4=C1.[I-]

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 5 mg/mL (7.67 mM; ultrasonic and warming and heat to 60°C)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5332 mL 7.6660 mL 15.3320 mL
    5 mM 0.3066 mL 1.5332 mL 3.0664 mL
    10 mM --- --- ---
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: 1.25 mg/mL (1.92 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 1.25 mg/mL (1.92 mM); Suspended solution; Need ultrasonic

    *All of the co-solvents are available by MCE.
    Purity & Documentation
    References
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    Product Name:
    JC-1
    Cat. No.:
    HY-15534
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