1. Dye Reagents

JC-1 (Synonyms: CBIC2)

Cat. No.: HY-15534 Purity: >95.0%
Data Sheet SDS Handling Instructions

JC-1, a fluorescent lipophilic carbocyanine dye, that can be used to measure mitochondrial membrane potential.

For research use only. We do not sell to patients.
JC-1 Chemical Structure

JC-1 Chemical Structure

CAS No. : 3520-43-2

Size Price Stock Quantity
10 mM * 1 mL in DMSO $215 In-stock
2 mg $80 In-stock
5 mg $150 In-stock
10 mg $250 In-stock
50 mg $1067 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Customer Validation

    JC-1 purchased from MCE. Usage Cited in: New J Chem. 18 Oct 2017.

    Measurement of mitochondria membrane potential by JC-1 staining. HeLa cells are incubated with or without WOs for 24 h, followed by NIR irradiation for NIR and WOs + NIR groups.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    JC-1, a fluorescent lipophilic carbocyanine dye, that can be used to measure mitochondrial membrane potential.

    In Vitro

    JC-1 (2.5 μM) exposed to murine L1210 lymphoblasts, can be detected the presence of both cytoplasmic JC-1 monomer and mitochondrial J-aggregates in these cells. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers[1]. Fluorescent labeling of mitochondria with either JC-1 (1 μg/mL, 15 min), reveals that are distributed irregularly, resulting in regions of high and low mitochondrial content within astrocytes[2]. JC-1 has been shown to interact with α-synuclein at the acidic C-terminal region with a Kd of 2.6 μM. JC-1 itself does not accelerate the protein aggregation of α-synuclein in the absence of iron, insted, it decelerates the aggregation process by extending the lag phase approx[3]. JC-1 is avidly accumulated in sensitive K562 cells where it displays both a green cytoplasmic and red mitochondrial fluorescence. JC-1 is poorly accumulated in resistant K562 cells, which displays only a slight green fluorescence[4].

    References
    Molecular Weight

    652.23

    Formula

    C₂₅H₂₇Cl₄IN₄

    CAS No.

    3520-43-2

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    10 mM in DMSO

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    Purity: >95.0%

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    Product Name:
    JC-1
    Cat. No.:
    HY-15534
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