1. Dye Reagents
  2. JC-1

JC-1 (Synonyms: CBIC2)

Cat. No.: HY-15534 Purity: ≥99.0%
Handling Instructions

JC-1 (CBIC2) is a fluorescent lipophilic carbocyanine dye used to measure mitochondrial membrane potential. JC-1 forms complexes known as J-aggregates at high ΔΨm. Aggregates of JC-1 emit an orange-red fluorescence (Ex/Em=585/590 nm). While in cells with low ΔΨm, JC-1 remains in the monomeric form. JC-1 monomers emit a green fluorescence (Ex/Em=510/527 nm).

For research use only. We do not sell to patients.

JC-1 Chemical Structure

JC-1 Chemical Structure

CAS No. : 3520-43-2

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5 mg USD 180 In-stock
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50 mg USD 1280 In-stock
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Customer Review

Based on 28 publication(s) in Google Scholar

Top Publications Citing Use of Products

    JC-1 purchased from MCE. Usage Cited in: New J Chem. 2017 41(23).

    Measurement of mitochondria membrane potential by JC-1 staining. HeLa cells are incubated with or without WOs for 24 h, followed by NIR irradiation for NIR and WOs + NIR groups.

    JC-1 purchased from MCE. Usage Cited in: Mol Cancer. 2019 Apr 10;18(1):85.

    KRA-533 induces caspase 3 activation and reduces mitochondrial membrane potential in NSCLC cells. The measurement of mitochondrial membrane potential by JC-1 staining.
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    Description

    JC-1 (CBIC2) is a fluorescent lipophilic carbocyanine dye used to measure mitochondrial membrane potential. JC-1 forms complexes known as J-aggregates at high ΔΨm. Aggregates of JC-1 emit an orange-red fluorescence (Ex/Em=585/590 nm). While in cells with low ΔΨm, JC-1 remains in the monomeric form. JC-1 monomers emit a green fluorescence (Ex/Em=510/527 nm).

    In Vitro

    Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
    Labeling of Cells:
    1. Culture cells in 6-, 12- , 24-, or 96-well plates at a density of 5× 105 cells/mL. Incubate the cells according to your normal protocol.
    2. Ensure that the JC-1 and DMSO has equilibrated to room temperature, and then prepare a 200 μM stock solution by dissolving the contents of one vial in DMSO provided.
    3. For the control tube, allow the vial of CCCP has come to room temperature, add 1 μL of CCCP (50 mM). Incubate cells at 37°C for 5 minutes.
    4. Add 10 μL JC-1 (200 μM) per well to make the final concentration at 2 μM. Incubate cells at 37°C, 5% CO2, for 15-20 minutes. If additional labeling followed, for example with an annexin V, begin with step 2.a. If not, proceed with step 1.e.
    5. After incubation, centrifuge cells for 3-4 minutes at 400× g at 4°C, carefully aspirate the supernant.
    6. Wash cells twice with PBS (1×): add 2 mL PBS (1×) to suspend cells and vortex to mix thoroughly. Centrifuge cells for 3-4 minutes at 400× g at 4°C, carefully aspirate the supernant.
    7. Add 500 μL PBS (1×) to suspend cells. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    652.23

    Formula

    C₂₅H₂₇Cl₄IN₄

    CAS No.
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 15 mg/mL (23.00 mM)

    H2O : < 0.1 mg/mL (insoluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5332 mL 7.6660 mL 15.3320 mL
    5 mM 0.3066 mL 1.5332 mL 3.0664 mL
    10 mM 0.1533 mL 0.7666 mL 1.5332 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: 1.25 mg/mL (1.92 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 1.25 mg/mL (1.92 mM); Suspended solution; Need ultrasonic

    *All of the co-solvents are provided by MCE.
    References
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    • Molarity Calculator

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    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

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    Product Name:
    JC-1
    Cat. No.:
    HY-15534
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