1. Dye Reagents
  2. JC-1

JC-1 (Synonyms: CBIC2)

Cat. No.: HY-15534 Purity: 99.46%
Handling Instructions

JC-1 is a fluorescent lipophilic carbocyanine dye used to measure mitochondrial membrane potential.

For research use only. We do not sell to patients.

JC-1 Chemical Structure

JC-1 Chemical Structure

CAS No. : 3520-43-2

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
2 mg USD 96 In-stock
Estimated Time of Arrival: December 31
5 mg USD 180 In-stock
Estimated Time of Arrival: December 31
10 mg USD 300 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1280 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Top Publications Citing Use of Products

    JC-1 purchased from MCE. Usage Cited in: New J Chem. 18 Oct 2017.

    Measurement of mitochondria membrane potential by JC-1 staining. HeLa cells are incubated with or without WOs for 24 h, followed by NIR irradiation for NIR and WOs + NIR groups.
    • Biological Activity

    • Technical Information

    • Purity & Documentation

    • References


    JC-1 is a fluorescent lipophilic carbocyanine dye used to measure mitochondrial membrane potential.

    In Vitro

    JC-1 (2.5 μM) exposed to murine L1210 lymphoblasts, can be detected the presence of both cytoplasmic JC-1 monomer and mitochondrial J-aggregates in these cells. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers[1]. Fluorescent labeling of mitochondria with either JC-1 (1 μg/mL, 15 min), reveals that are distributed irregularly, resulting in regions of high and low mitochondrial content within astrocytes[2]. JC-1 has been shown to interact with α-synuclein at the acidic C-terminal region with a Kd of 2.6 μM. JC-1 itself does not accelerate the protein aggregation of α-synuclein in the absence of iron, insted, it decelerates the aggregation process by extending the lag phase approx[3]. JC-1 is avidly accumulated in sensitive K562 cells where it displays both a green cytoplasmic and red mitochondrial fluorescence. JC-1 is poorly accumulated in resistant K562 cells, which displays only a slight green fluorescence[4].


    4°C, protect from light

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 15 mg/mL (23.00 mM)

    H2O : < 0.1 mg/mL (insoluble)

    *"≥" means soluble, but saturation unknown.

    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5332 mL 7.6660 mL 15.3320 mL
    5 mM 0.3066 mL 1.5332 mL 3.0664 mL
    10 mM 0.1533 mL 0.7666 mL 1.5332 mL
    *Please refer to the solubility information to select the appropriate solvent.
    Molecular Weight




    CAS No.





    Room temperature in continental US; may vary elsewhere

    Purity: 99.46%

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product name



    Applicant name *


    Email address *

    Phone number *


    Organization name *

    Country or Region *


    Requested quantity *


    Bulk Inquiry

    Inquiry Information

    Product Name:
    Cat. No.:


    Cat. No.: HY-15534