Dilution Calculator

In cell culture experiments, to calculate the stock solution volume (V1) given the stock concentration (C1), working concentration (C2), and working volume (V2):

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

C1
Concentration (start)
×
V1
Volume (start)
=
C2
Concentration (final)
×
V2
Volume (final)
An example of how to use the Dilution Calculator Equation

You purchased LY294002 and prepared a 10 mM stock solution for cell experiments. The working concentration for the cell experiments is 10 μM, and you plan to prepare 10 mL of working solution. How much stock solution should you take each time?

  1. Enter 10 in the C1 field and select the unit (mM)
  2. Enter 10 in the C2 field and select the unit (μM)
  3. Enter 10 in the V2 field and select the unit (mL)
  4. Click "Calculate"
  5. The result is 10 μL. It is recommended to take 10 μL of the stock solution, add it to 9990 μL of culture medium, mix well, and then add to the cells via a medium change.
How do I dilute the compound solution for cell assay?
Stock solution using ddH2O as a solvent can be directly diluted with medium to prepare the working solution. When DMSO is used to prepare the stock solution, the stock solution is diluted in the culture medium to prepare a working solution. Make sure the concentration of DMSO is <0.5% to avoid poisoning the cells. A negative control in the experiment is usually the culture medium with DMSO at the same concentration. It is recommended that the process of dilution is be performed in a stepwise manner to avoid compound precipitating caused by fast change of concentration.