MEM-α Medium (with Nucleosides)
MEM-α 是由 Harry Eagle 于 20 世纪 50 年代研发的 Eagle's Basal Medium (BME) 经改良发展而来的合成细胞培养基之一，是目前应用最广泛的哺乳动物细胞培养基之一。1971年 Stanners 等人在 MEM 基础上进一步提高了氨基酸种类与浓度、增补维生素组分，形成 MEM-α (alpha modification)。MEM-α 在标准 MEM 基础上补充了非必需氨基酸、丙酮酸钠、硫辛酸、维生素 B12、生物素、抗坏血酸等成分，营养更全面，适用细胞谱更广 (涵盖悬浮与贴壁两类细胞)，是目前 MEM 系列中营养强化的改良版本。
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Storage :
2–8°C, 1 year. Protect from light.
- Manual (1946KB)
- COA
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Description & Advantages
MEM-α is a synthetic cell culture medium developed through modifications of Eagle's Basal Medium (BME), which Harry Eagle formulated in the 1950s. It is now among the most widely used media for mammalian cell culture. In 1971, Stanners and colleagues further modified MEM by increasing the range and concentrations of amino acids and adding vitamins, creating MEM-α (alpha modification). Compared with standard MEM, MEM-α contains additional components, including non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. Its broader nutrient profile supports a wider range of cells, including both suspension and adherent cells, making it a nutrient-enriched formulation in the MEM family.
MCE MEM-α Medium (with Nucleosides) is the standard formulation containing ribonucleosides and deoxyribonucleosides. Its nutrient profile provides cells with these components directly, reducing their reliance on de novo nucleotide synthesis. It is widely suitable for the routine culture of various mammalian cells.
| Contains | Does Not Contain |
|---|---|
| Ribonucleosides | HEPES |
| Deoxyribonucleosides | |
| L-Glutamine (2 mM) | |
| Phenol Red (10.0 mg/L) |
Protocols
MCE MEM-α Medium (with Nucleosides) is a basal medium that does not contain proteins, lipids, or growth factors. Add appropriate supplements according to the cell type and experimental requirements. For routine culture, approximately 10% fetal bovine serum (Cat. No.: HY-T1000) may be added. For low-serum or serum-free culture, supplements such as insulin-transferrin-selenium (ITS, Cat. No.: HY-K3018) may be added as needed.
This product contains L-Glutamine, which gradually degrades during storage in liquid medium. If the medium has been stored at 2–8°C for an extended period, assess whether additional L-Glutamine is needed based on the cells’ requirements and culture performance.
Note: This product does not contain antibiotics. Penicillin-Streptomycin Solution (Cat. No.: HY-K1006) may be added if required by the experiment. After adding serum, ITS, antibiotics, or other supplements, store the prepared medium at 2–8°C and use it within one week.
Application
MCE MEM-α Medium (with Nucleosides) is suitable for culturing a wide range of suspension and adherent cells, including HeLa, BHK-21, HEK 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. This product contains ribonucleosides and deoxyribonucleosides, providing cells with readily available nucleoside precursors and reducing the need for de novo synthesis. Its nutrient-rich formulation is particularly suitable as a basal medium for routine culture during cell recovery and expansion.
Storage
2–8°C, 1 year.
Protect from light.
Attention
1. This product has been sterilized by filtration. Use aseptic technique to avoid contamination.
2. When using this product for cell culture, place the cells in a temperature-controlled incubator with 5%–10% CO₂ to maintain an appropriate growth environment.
3. This product is intended for scientific research by trained personnel only. It must not be used for clinical diagnosis or treatment, or in food or pharmaceutical products.
4. For your safety and health, wear a lab coat and disposable gloves when handling this product.
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