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Cat. No.: HY-K2035

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Fluc mRNA (N1-Me-pUTP)

MCE Fluc mRNA (N1-Me-pUTP) is widely used for evaluating lipid nanoparticle (LNP) delivery efficiency, validating mRNA transfection reagents, assessing in vitro and in vivo mRNA expression, IVIS bioluminescence imaging, and screening novel nucleic acid delivery systems. It is commonly used as a positive control or reporter mRNA.

Storage :
-20°C, 1 year.

Manual (1166KB)
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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Description & Advantages

Firefly Luciferase (Fluc) mRNA (N1-methyl-pseudouridine-modified) is an in vitro transcribed (IVT) mRNA encoding Firefly Luciferase (Fluc), in which uridine triphosphate (UTP) is completely or partially replaced with N1-methyl-pseudouridine triphosphate (N1-Me-pUTP, also referred to as m1ΨTP). Incorporation of N1-methyl-pseudouridine reduces the innate immunogenicity of mRNA while enhancing its stability and protein expression.

Protocol

1. Before opening the vial, briefly centrifuge at 2,000–4,000 g for 3 min to collect the lyophilized mRNA at the bottom of the tube.

2. Reconstitute the mRNA with Nuclease-Free Water to prepare a stock solution at a concentration of ≥1 μg/μL. Aliquot the stock solution according to the amount required for each experiment.

Note: a. The reconstituted mRNA stock solution may be stored at −80°C for up to 6 months. Repeated freeze-thaw cycles should be avoided to preserve mRNA integrity and biological activity.

b. Throughout the procedure, use only RNase-free reagents, consumables, and laboratory equipment to prevent RNase contamination and subsequent mRNA degradation.