1. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (947):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-D0940
    H2DCFDA 4091-99-0 ≥98.0%
    H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm)[1].
  • HY-15534
    JC-1 3520-43-2 ≥99.0%
    JC-1 (CBIC2) is a fluorescent lipophilic carbocyanine dye used to measure mitochondrial membrane potential.
  • HY-D0938
    CFDA-SE 150347-59-4
    CFDA-SE (5(6)-Carboxyfluorescein diacetate succinimidyl ester) is an intracellular fluorescent dye which can track proliferating cells.
  • HY-D0718
    Nile Red 7385-67-3 98.02%
    Nile Red (Nile Blue A oxazone) is a selective and hydrophobic fluorescent stain for intracellular lipid droplets and neutral lipids.
    Nile Red
  • HY-15924
    Thiazolyl Blue 298-93-1 ≥98.0%
    Thiazolyl Blue (MTT) is a colorimetric agent widely used to measure cell proliferation.
    Thiazolyl Blue
  • HY-D0815
    Propidium Iodide 25535-16-4 ≥98.0%
    Propidium Iodide is a red-fluorescent dye that can be used to stain cells.
    Propidium Iodide
  • HY-66019
    FITC 3326-32-7 ≥98.0%
    FITC (Fluorescein 5-isothiocyanate) is a fluorescence probe for the labeling of amines.
  • HY-12591B
    D-Luciferin potassium 115144-35-9 ≥98.0%
    D-Luciferin (D-(-)-Luciferin) potassium is the substrate of luciferases that catalyze the production of light in bioluminescent insects[1].
    D-Luciferin potassium
  • HY-D0041
    Calcein-AM 148504-34-1 ≥98.0%
    Calcein-AM is cell-permeable fluorescent dye used to determine the cell viability.
  • HY-N6716
    Filipin complex 11078-21-0
    Filipin complex is a potent broad spectrum 28-membered ring pentaene macrolide antifungal antibiotic.
    Filipin complex
  • HY-128868A
    FITC-Dextran (MW 4000) 60842-46-8
    FITC-Dextran (MW 4000) is a marker consisting of coupling fluorescein-isothiocyanate to dextran (4 kDa).
    FITC-Dextran (MW 4000)
  • HY-128868
    FITC-Dextran (MW 10000) 60842-46-8
    FITC-Dextran (MW 10000) is a marker consisting of coupling fluorescein-isothiocyanate to dextran (10 kDa).
    FITC-Dextran (MW 10000)
  • HY-D0083
    DiI 41085-99-8 ≥98.0%
    DiI (DiIC18(3)) is a lipophilic carbocyanine fluorescent dye for membrane labeling.
  • HY-12591A
    D-Luciferin 2591-17-5 99.87%
    D-Luciferin (D-(-)-Luciferin) is the substrate of luciferases that catalyze the production of light in bioluminescent insects[1].
  • HY-D0814
    DAPI dihydrochloride 28718-90-3 ≥98.0%
    DAPI dihydrochloride (4',6-diamidino-2-phenylindole) is a fluorescent stain by binding in the minor grove of A-T rich sequences of DNA.
    DAPI dihydrochloride
  • HY-D0079
    Dihydroethidium 104821-25-2
    Dihydroethidium(Hydroethidine; PD-MY 003) is a superoxide indicator; exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red (Ex/Em=518/616 nm).
  • HY-D0985A
    TMRE 115532-52-0 ≥98.0%
    TMRE is a mitochondria specific dye (λex=550 nm, λem=575 nm).
  • HY-D0218
    Thioflavin T 2390-54-7 ≥98.0%
    Thioflavin T is a cationic Benzothiazole dye that shows enhanced fluorescence upon binding to amyloid in tissue sections.
    Thioflavin T
  • HY-D0711
    Indocyanine green 3599-32-4 ≥98.0%
    Indocyanine green (Foxgreen) is a low toxicic fluorescent agent that has been widely used in medical diagnostics, such as determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography.
    Indocyanine green
  • HY-101896
    Fluo-4 AM 273221-67-3 ≥99.0%
    Fluo-4 AM is a cell-permeable Ca2+ indicator[1].
    Fluo-4 AM