Cell Culture Supplements

Cell Culture Supplements are essential components of cell culture systems, providing nutrients and bioactive factors required for cell growth, proliferation, differentiation, and maintenance of cellular functions. These supplements include L-glutamine, L-alanyl-L-glutamine, D-glucose, sodium pyruvate, MEM Vitamins, Non-Essential Amino Acids (NEAA), and other culture additives. Appropriate supplementation according to specific cell types and culture requirements helps optimize culture conditions, improve cell viability and stability, and enhance experimental reproducibility.

MCE offers a comprehensive portfolio of Cell Culture Supplements for a wide range of applications, including stem cell culture, primary cell culture, neuronal cell culture, immune cell culture, organoid culture, and other mammalian cell culture systems, providing reliable support for cell culture optimization and life science research.

Cat. No.: HY-K3011 MEM Non-essential Amino Acid Solution (100×)

MEM Non-essential Amino Acid Solution (100×) has been filtered and can be used directly. It adds nutrients and reduces the biosynthetic burden on cell in vitro. The 100 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K1046 L-Glutamine (100×), Sterile

L-glutamine is an important amino acid supplement commonly added to mammalian cell culture media. L-glutamine serves as an auxiliary energy source, especially when cells are rapidly dividing. L-glutamine is also important in the production of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, other amino acids, and plays a role in protein synthesis and glucose production.

Cat. No.: HY-K3012 N-2 Supplement (100×), Sterile

This product is serum-free and supports cell growth and survival. It has been associated with the growth and expression of neuroblastoma, and with post-mitotic neuronal survival and expression in primary cultures of both the peripheral nervous system (PNS) and central nervous system (CNS). This product is formulated with water-for-injection. The 5 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K3013 Bi-27 Supplement (50×), Sterile

This product is the serum-free B-27 Supplement, the most widely cited neuronal culture supplement. It is an optimized formulation designed to support both low- and high-density growth, as well as short- and long-term viability of embryonic neurons. This product is formulated with water-for-injection. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K3014 Bi-27 Supplement (50×), Vitamin A-Free, Sterile

This product is serum-free and vitamin A-free.It is a customized variant of the standard Bi-27 formulation with vitamin A removed as a supplement for neuronal cell culture to support the low- or high-density growth and short- or long-term viability of embryonic. This product is formulated with water-for-injection. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K3012A N-2 Serum-free Supplement (100×)

MCE N-2 Serum-free Supplement (100×) can be used in conjunction with growth factors such as bFGF and EGF in serum-free neuronal cell culture mediums, DMEM and the DMEM/F12. This product is formulated with basal culture medium. The 5 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K3013A Bi-27 Serum-free Supplement (50×)

MCE Bi-27 Serum-free Supplement (50×) can be used to support the low- or high-density growth and short- or long-term viability of embryonic. This product is formulated with basal culture medium. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K3014A Bi-27 Serum-free Supplement (50×), Vitamin A-Free

MCE Bi-27 Serum-free Supplement (50×), Vitamin A-Free can be used to support the low- or high-density growth and short- or long-term viability of embryonic. This product is formulated with basal culture medium. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K3016 HAT Supplement (50×)

MCE HAT Supplement (50×) can be added to cell culture medium as a selection agent for post-fusion selection of HGPRT-myeloma hybridoma cells used for antibody production. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K3017 HT Supplement (50×)

MCE HT Supplement (50×) is a liquid mixture of hypoxanthine (5 mM) and thymidine (0.8 mM), and can be added to cell culture medium as an additive for the DNA salvage synthesis pathway. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K3018 ITS Supplement (100×)

MCE ITS Supplement (100×) can be used as a cell culture supplement and added to the medium for serum-free or low-serum cell culture systems.

Cat. No.: HY-K3019 Lipid Concentrate (Chemically Defined)

MCE Lipid Concentrate (Chemically Defined) is a concentrated lipid solution with a clearly defined chemical composition, which can be added to cell culture media as an additive for serum-free or low-serum cell culture systems.

Cat. No.: HY-K3114 Exosome-Specific 293 Cell Medium Supplement

MCE Exosome-Specific 293 Cell Medium Supplement is designed as a feed supplement for use with the Exosome-Specific 293 Cell Medium (Serum-Free) (MCE Cat. No.: HY-K3113).

Cat. No.: HY-K3022 Recombinant Human Serum Albumin Solution (rHSA, 20%)

MCE Recombinant Human Serum Albumin Solution (rHSA, 20%) can be widely used in various cell culture systems and is particularly suitable for maintaining the undifferentiated growth and expansion of human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC) under both feeder-dependent and feeder-free conditions.

Cat. No.: HY-K3014C Bi-27 Serum-free Supplement (50×), Insulin-Free

MCE Bi-27 Serum-free Supplement (50×), Insulin-Free is a customized formulation developed by removing insulin from the standard Bi-27 formulation. As a neuronal cell culture supplement, it is specifically designed to support the growth and maintenance of hippocampal neurons and other central nervous system (CNS) neurons in applications where the presence of insulin may interfere with experimental outcomes.

Cat. No.: HY-K3014D Bi-27 Serum-free Supplement (50×), Antioxidant-Free

MCE Bi-27 Serum-free Supplement (50×), Antioxidant-Free is a customized neuronal culture supplement developed by removing antioxidants components (such as vitamins, superoxide dismutase, catalase, etc.) from the standard Bi-27 formulation. It is specifically designed for investigating the roles of reactive oxygen species (ROS) in aging, cytotoxicity, apoptosis, and chronic neurological diseases. This formulation provides a stable culture environment for mechanistic studies without the interference of exogenous antioxidants.

Cat. No.: HY-K1046P L-Alanyl-L-Glutamine (100×)

MCE L-Alanyl-L-Glutamine (100×) contains 200 mM L-alanyl-L-glutamine and is formulated in 0.85% NaCl. It serves as a stable dipeptide substitute for L-glutamine. Compared with free L-glutamine, it exhibits superior stability and significantly reduces the accumulation of toxic ammonia during culture, thereby improving cell viability and growth performance.

Cat. No.: HY-K3024 Sodium Pyruvate Solution (100 mM)

MCE Sodium Pyruvate Solution (100 mM) contains 100 mM (11 g/L) sodium pyruvate and is ready for direct use in cell culture applications. The recommended working concentration is 1 mM, corresponding to a 1:100 dilution prior to use.

Cat. No.: HY-K3025 D-Glucose Solution (200 g/L)

MCE D-Glucose Solution (200 g/L) can be used as a serum-free supplement for cell culture media and is suitable for a variety of culture systems, including but not limited to DMEM, RPMI 1640, DMEM/F-12, IMDM, and MEM.

Cat. No.: HY-K3026 MEM Vitamine Solution (100×)

MCE MEM Vitamin Solution (100×) contains eight vitamins, including choline chloride, D-calcium pantothenate, folic acid, nicotinamide, pyridoxal hydrochloride, riboflavin, thiamine hydrochloride, and inositol. It can be used as a serum-free supplement for cell culture media to enhance cell viability and promote cell growth.

Cat. No.: HY-K1054B HEPES (1 M, Cell Culture Grade)

MCE HEPES (1 M, Cell Culture Grade) is prepared using high-purity HEPES and sterilized by 0.22 μm membrane filtration. It can be added directly to sterile cell culture media for cell culture and other related applications.