Mouse Tissue Direct PCR Kit (with Dye) 

MCE Mouse Tissue Direct PCR Kit (with Dye) is specifically designed for direct PCR amplification from tissue samples. It enables rapid, one-step release of genomic DNA from various mouse tissues (such as tail, ear, toe, and muscle), which can be directly used for downstream PCR amplification and analysis without the need for conventional DNA extraction and purification steps, thereby significantly simplifying the experimental workflow.

This kit utilizes an optimized lysis system, requiring only 15 min for tissue digestion to generate PCR-ready DNA templates. In addition, the provided PCR Master Mix contains all essential components for PCR amplification, including Taq DNA polymerase, dNTPs, reaction buffer, and loading dye. Users only need to add the template and primers to perform amplification. The PCR products can be directly analyzed by agarose gel electrophoresis without the need for additional loading buffer, effectively reducing operational complexity and the risk of contamination. It is particularly suitable for mouse genotyping and transgenic identification, and recommended for amplification of targets within 1 kb.

Features of MCE Mouse Tissue Direct PCR Kit (with Dye):

1. Fast and Convenient: One-step DNA preparation from mouse tissues; template preparation completed within 10–15 min, and PCR amplification within 1 h.

2. Low Sample Requirement: Only ~5 mg tissue or 1–5 mm mouse tail is sufficient.

3. High Specificity and Efficiency: Optimized Taq polymerase system with strong template affinity and high amplification specificity, ideal for genotyping and transgenic analysis.

4. User-Friendly Operation: The master mix contains tracking dyes, allowing PCR products to be directly loaded for electrophoresis.

5. High-Throughput Compatibility: Lysis and PCR can be performed in 96-well plates, suitable for large-scale mouse sample screening.

Lysis Buffer: 4°C, 1 year. If not used for an extended period, it is recommended to aliquot and store at low temperature to avoid repeated freeze–thaw cycles.

Lysis Stop Solution, Direct PCR Master Mix (2×, with Dye): -20°C, 1 year. Avoid repeated freeze-thaw cycles.

1. To prevent cross-contamination between samples, sampling tools should be thoroughly cleaned after each use. It is recommended to immerse the cutting edge or any part in direct contact with the sample in 2% sodium hypochlorite solution, rinse repeatedly, and then wipe dry with clean tissue before reuse. For improved efficiency, multiple sets of sampling tools may be prepared and cleaned collectively to ensure that each sample is handled with contamination-free instruments.

2. Freshly collected animal tissues are recommended for optimal results. For long-term frozen samples, repeated freeze–thaw cycles should be avoided, as they may lead to genomic DNA degradation and consequently affect PCR amplification efficiency and data reliability.

3. This product is for R&D use only, not for drug, household, or other uses.

4. For your safety and health, please wear a lab coat and disposable gloves to operate.