Exosome-Specific 293 Cell Medium (Serum-Free)
Based on 1 Customer Validation
MCE Exosome-Specific 293 Cell Medium (Serum-Free) is specifically optimized for exosome production from HEK293 cells.
-
Storage :
4°C, 1 year.
Protect from light
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
MCE Exosome-Specific 293 Cell Medium (Serum-Free) is specifically optimized for exosome production from HEK293 cells.
It features a chemically defined formulation, free of animal-derived components (Xeno-free), growth factors, and proteins. No serum supplementation is required during cell culture. When used in combination with the Exosome-Specific 293 Cell Medium Supplement (MCE Cat. No.: HY-K3114), it significantly enhances exosome secretion from 293 cells, achieving a 5-10 fold increase in yield compared to conventional HEK293 culture media.
Protocol
1. Pre-warm the Exosome-Specific 293 Cell Medium (Serum-Free) to 37°C.
2. Prepare HEK293 cells in the logarithmic growth phase with ≥ 95% viability at a density of 2 × 106 - 1 × 107 cells/mL.
3. Inoculate the cells into the pre-warmed Exosome-Specific 293 Cell Medium (Serum-Free) at a seeding density of 3 × 105 - 5 × 105 cells/mL.
4. Culture the cells in sterile shake flasks or a bioreactor.
Shake flask culture conditions: 37°C, 80% humidity, 5%-10% CO2, 140 rpm.
Bioreactor culture conditions: 37°C, pH 7.0, DO 40%, stirring speed 160 rpm.
5. Expand the HEK293 suspension cells until the density reaches 6 × 106 - 8 × 106 cells/mL.
Note: To further enhance exosome production, use the Exosome-Specific 293 Cell Medium Supplement (MCE Cat. No.: HY-K3114). Add the supplement at 25% of the culture volume. Continue culturing for 6-16 h, and collect the culture supernatant before cell viability drops below 90%.
6. The collected culture supernatant can be used for exosome isolation and purification.
Storage
4°C, 1 year.
Protect from light
Attention
1. This product is a sterile solution, pay attention to aseptic operation to avoid contamination.
2. This product contains phenol red, L-glutamine, and sodium bicarbonate.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.
Please enter the correct Lot Number
