2. MCE Kits
  3. Molecular Biology
  4. PCR & qPCR
  5. Real-Time PCR

Real-Time PCR

FAQ

qPCR (Quantitative PCR) kits are high-sensitivity products designed for quantitative nucleic acid analysis. By monitoring fluorescence signals in real time during amplification, they enable accurate quantification of target nucleic acids.

 

MCE qPCR kits utilize optimized enzyme systems and fluorescence detection chemistry (dye-based), delivering high amplification efficiency with low background signals to ensure data accuracy and reproducibility. They are well suited for applications such as gene expression analysis, pathogen detection, and copy number variation studies.

Cat. No.
Name
Description
  • HY-K0501 SYBR Green qPCR Master Mix

    MCE qPCR Master Mix is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water. The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
    HY-K0501A SYBR Green qPCR Master Mix (Universal)

    MCE SYBR Green qPCR Master Mix (Universal) is compatible with all qPCR instruments. 2× ready-to-use formulations which consist of antibody-mediated hot start polymerase, MgCl2, dNTPs and a proprietary reaction buffer, produce optimal results in qPCR experiments. The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
    HY-K0521 SYBR Green qPCR Master Mix (High ROX)

    MCE SYBR Green qPCR Master Mix (High ROX) is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water, in which High ROX Reference Dye is included. The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
    HY-K0522 SYBR Green qPCR Master Mix (Low ROX)

    MCE SYBR Green qPCR Master Mix (Low ROX) is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water, in which Low ROX Reference Dye is included. The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
    HY-K0523 SYBR Green qPCR Master Mix (No ROX)

    MCE SYBR Green qPCR Master Mix (No ROX) is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water, in which no ROX Reference Dye is included. The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
    HY-K0524 2-Color Tracking SYBR qPCR Master Mix (Universal)

    MCE 2-Color Tracking SYBR qPCR Master Mix (Universal) is a 2× concentration premixed reagent utilizing a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR (qPCR). The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.