β-glucuronidase

GUSB

β-glucuronidase is a glycoside hydrolase widely distributed in mammalian tissues, body fluids, microbiota, plants, fish, insects and mollusks. β-glucuronidase belongs to GH family 1, 2, 30, 79, 154 and GH-A. β-glucuronidase can hydrolyze estrogen glucuronides, regulate the enterohepatic circulation and reabsorption of estrogen, and affect estrogen metabolism. β-glucuronidase can also hydrolyze the extracellular sugar residues on the TRPV5 channel, trapping it in the plasma membrane, maintaining calcium channel activity and membrane calcium permeability. This target is related to many disease areas. For example, in cancer, its increased activity is related to the occurrence and development of colorectal cancer, breast cancer, ovarian cancer, etc.; in metabolic diseases, it is related to diabetes and its induced periodontitis; in inflammatory diseases, it involves bacterial peritonitis, bacterial meningitis, pelvic inflammatory disease, etc.; in addition, it is also related to diseases such as endometriosis^[1]^[2]^[3]^[4]^[5]^[6]^[7].

References:

[1]. Wei Y, et al. Gut dysbiosis-derived β-glucuronidase promotes the development of endometriosis. Fertil Steril. 2023 Sep;120(3 Pt 2):682-694. [Content Brief]

[2]. Awolade P, et al. Therapeutic significance of β-glucuronidase activity and its inhibitors: A review. European Journal of Medicinal Chemistry, 2020, 187: 111921.

[3]. Kim DH, et al. Intestinal bacterial beta-glucuronidase activity of patients with colon cancer. Arch Pharm Res. 2001 Dec;24(6):564-7. [Content Brief]

[4]. Chang Q, et al. The beta-glucuronidase klotho hydrolyzes and activates the TRPV5 channel. Science. 2005 Oct 21;310(5747):490-3. [Content Brief]

[5]. Sui Y, et al. The Role of Gut Microbial β-Glucuronidase in Estrogen Reactivation and Breast Cancer. Front Cell Dev Biol. 2021 Aug 12;9:631552. [Content Brief]

[6]. Liu Z, et al. A novel allosteric driver mutation of β-glucuronidase promotes head and neck squamous cell carcinoma progression through STT3B-mediated PD-L1 N-glycosylation. MedComm (2020). 2025 Jan 19;6(2):e70062. [Content Brief]

[7]. Paigen K. Mammalian beta-glucuronidase: genetics, molecular biology, and cell biology. Prog Nucleic Acid Res Mol Biol. 1989;37:155-205. [Content Brief]

β-glucuronidase Related Products (46):

By Effects:	Inhibitors (25) Substrates (10) Activators (2) Chemical (1)

Cat. No.	Product Name	Effect	Purity	Chemical Structure

HY-134453A

D-Saccharic acid 1,4-lactone hydrate	Inhibitor	99.97%
D-Saccharic acid 1,4-lactone hydrate is an orally active β-glucuronidase inhibitor (IC₅₀=48.4 μM). D-Saccharic acid 1,4-lactone hydrate can be used as a standard agent compared with novel β-glucuronidase inhibitors. D-Saccharic acid 1,4-lactone hydrate possesses anticarcinogenic, detoxifying, and antioxidant properties.

HY-D0935A

4-Methylumbelliferyl-β-D-glucuronide hydrate	Substrate	99.92%
4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λ_ex=362 nm, λ_em=445 nm). 4-Methylumbelliferyl-β-D-glucuronide hydrate has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli.

HY-P2803

Beta-glucuronidase (Escherichia coli)
Beta-glucuronidase is a glycosyl hydrolase that hydrolyzes β-glucuronic acid and sulfate esters in urine and other biological fluids, and then releases β-glucuronate.

HY-W345885

Saccharic acid	Inhibitor	98.73%
Saccharic acid is a competitive and potent inhibitor of β-glucuronidase. Saccharic acid inhibits glucuronide synthesis. Saccharic acid as an efficient iron chelate to enhance photo-Fenton degradation of organic contaminants.

HY-W039892

4-Nitrophenyl β-D-glucuronide	Substrate	99.87%
4-Nitrophenyl β-D-glucuronide (pNPG_1) is aderivative of 4-Nitrophenol. 4-Nitrophenyl β-D-glucuronide is the chromogenic substrate for β-glucuronidase.

HY-P2803D

β-Glucuronidase, Abalone
β-Glucuronidase, Abalone is a glycosyl hydrolase that hydrolyzes β-glucuronic acid and sulfate esters in urine and other biological fluids, and then releases β-glucuronate.

HY-153875

Saccharolactone	Inhibitor
Saccharolactone is a potent orally active β-glucuronidase inhibitor. Saccharolactone markedly lowers biliary endogenous β-glucuronidase activity in the rat bile. Saccharolactone can stabilize glucuronide metabolites in vitro. Saccharolactone is also a strong inhibitor of CYP1A2, 2D6, 3A4 and 2C8 isoforms (IC₅₀ < 4 mM).

HY-W472153

β-Glucuronidase-IN-5	Inhibitor
β-Glucuronidase-IN-5 (Compound 19; Compound 5c) is a β-glucuronidase inhibitor with an IC₅₀ of 39.8 μM. β-Glucuronidase-IN-5 does not show significant cytotoxicity towards PC-3 cells, with its IC₅₀ > 30 μM. β-Glucuronidase-IN-5 shows no affinity for adenosine receptors (A₁ and A₂A receptors). β-Glucuronidase-IN-5 can be used to study diseases related to excessive expression of β-glucuronidase (such as colon cancer, arthritis, and AIDS complications).

HY-P2803A

Beta-glucuronidase (bovine liver)
Beta-glucuronidase (bovine liver) is a glycosyl hydrolase that hydrolyzes β-glucuronic acid and sulfate esters in urine and other biological fluids, and then releases β-glucuronate.

HY-103081

β-Glucuronidase-IN-1	Inhibitor	98.21%
β-Glucuronidase-IN-1 is a potent, selective, uncompetitive, and orally active E. coli bacterial β-glucuronidase inhibitor, exhibiting an IC₅₀ and a K_i of 283 nM and 164 nM, respectively.

HY-15935B

X-Gluc cyclohexanamine		99.84%
X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue).

HY-P2803B

Beta-glucuronidase (helix pomatia)	Chemical
Beta-glucuronidase (helix pomatia) is a glycosyl hydrolase that hydrolyzes β-glucuronic acid and sulfate esters in urine and other biological fluids, and then releases β-glucuronate.

HY-148044

UNC10201652	Inhibitor	99.33%
UNC10201652 is a potent Loop 1 (L1)-specific gut bacterial β-glucuronidase (GUSs) inhibitor with an IC₅₀ value of 0.117 μM for E. coli GUS. UNC10201652 can block SN-38 glucuronide (HY-126373) processing only in individuals whose fecal gut microbiota is highly abundant in L1 GUS enzymes.

HY-N1733

2′-Hydroxydaidzein	Inhibitor
2′-Hydroxydaidzein is a metabolite. 2′-Hydroxydaidzein inhibits the release of chemical mediator from inflammatory cells. 2′-Hydroxydaidzein significantly inhibits lysozyme and β-glucuronidase release from rat neutrophils, which is stimulated with fMLP/CB, respectively.

HY-15935C

X-Gluc sodium		98.0%
X-Gluc sodium is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue).

HY-151168

3-Chlorogentisyl alcohol	Inhibitor	99.70%
3-Chlorogentisyl alcohol is a potent E. coli β-glucuronidase inhibitor with an IC₅₀ value of 0.74 μM, an K_i value of 0.58 μM. 3-Chlorogentisyl alcohol shows antiproliferative activity. 3-Chlorogentisyl alcohol has the potential for the research of anti-cancer and anti-inflammatory therapies.

HY-D1460

Fluorescein Di-β-D-Glucuronide
Fluorescein Di-β-D-Glucuronide is an orally active fluorescent probe substrate and an indicator of β-glucuronidase (βG) activity in intestinal bacteria. Fluorescein Di-β-D-Glucuronide can be used for non-invasive time-lapse optical imaging of βG activity in intestinal bacteria in nude mice. Fluorescein Di-β-D-Glucuronide supports the quantitative analysis of bacterial βG enzyme activity and inhibition.

HY-D0164

Naphthol AS-BI	Substrate	99.86%
Naphthol AS-BI is a substrate of β-glucuronidase and produces a bright red effect after staining biological tissues.

HY-N1735

2′-Hydroxygenistein	Inhibitor	99.58%
2′-Hydroxygenistein is a natural product that can be isolated from Crotalaria pallida and C. assamica. 2′-Hydroxygenistein shows anti-inflammatory activity. 2′-Hydroxygenistein shows significant concentration-dependent inhibitory effects on the release of β-glucuronidase and lysozyme from rat neutrophils, with IC₅₀ values of 5.9 ± 1.4 and 9.7 ± 3.5 μM, respectively.

HY-D0935

4-Methylumbelliferyl-β-D-glucuronide	Substrate	99.92%
4-Methylumbelliferyl-β-D-glucuronide is a fluorogenic substrat (λ_ex=362 nm, λ_em=445 nm). 4-Methylumbelliferyl-β-D-glucuronide has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli.

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