X-Gluc cyclohexanamine
Based on 1 Customer Validation
X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue).
For research use only. We do not sell to patients.
- Purity: 99.64%
- CAS No.: 114162-64-0
- Formula: C20H26BrClN2O7
- Molecular Weight:521.79
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolve 20 mg X-Gluc in 1mL Dimethylformamide (DMF) to prepare X-Gluc master mix.
2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization.
3. Allow the plates to air dry and be used to inoculate the organisms.
4. Incubate the plates at 35°C and observe 16-24 h after inoculation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 114162-64-0
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Appearance Solid
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Molecular Weight 521.79
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Formula C20H26BrClN2O7
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Color White to off-white
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SMILES
NC1CCCCC1.O[C@H]([C@@H](O)[C@@H]2O)[C@@H](O[C@@H]2C(O)=O)OC3=CNC4=C3C(Cl)=C(Br)C=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL (191.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9165 mL | 9.5824 mL | 19.1648 mL | 47.9120 mL |
| 5 mM | 0.3833 mL | 1.9165 mL | 3.8330 mL | 9.5824 mL | |
| 10 mM | 0.1916 mL | 0.9582 mL | 1.9165 mL | 4.7912 mL | |
| 15 mM | 0.1278 mL | 0.6388 mL | 1.2777 mL | 3.1941 mL | |
| 20 mM | 0.0958 mL | 0.4791 mL | 0.9582 mL | 2.3956 mL | |
| 25 mM | 0.0767 mL | 0.3833 mL | 0.7666 mL | 1.9165 mL | |
| 30 mM | 0.0639 mL | 0.3194 mL | 0.6388 mL | 1.5971 mL | |
| 40 mM | 0.0479 mL | 0.2396 mL | 0.4791 mL | 1.1978 mL | |
| 50 mM | 0.0383 mL | 0.1916 mL | 0.3833 mL | 0.9582 mL | |
| 60 mM | 0.0319 mL | 0.1597 mL | 0.3194 mL | 0.7985 mL | |
| 80 mM | 0.0240 mL | 0.1198 mL | 0.2396 mL | 0.5989 mL | |
| 100 mM | 0.0192 mL | 0.0958 mL | 0.1916 mL | 0.4791 mL |
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