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X-Gluc cyclohexanamine 

Cat. No.: HY-15935B
Handling Instructions

X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue).

For research use only. We do not sell to patients.

X-Gluc cyclohexanamine Chemical Structure

X-Gluc cyclohexanamine Chemical Structure

CAS No. : 114162-64-0

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Description

X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue)[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolve 20 mg X-Gluc cyclohexanamine in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix.
2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization.
3. Allow the plates to air dry and be used to inoculate the organisms.
4. Incubate the plates at 35°C and observe 16-24 h after inoculation.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

521.79

Formula

C20H26BrClN2O7

CAS No.
SMILES

NC1CCCCC1.O[[email protected]]([[email protected]@H](O)[[email protected]@H]2O)[[email protected]@H](O[[email protected]@H]2C(O)=O)OC3=CNC4=C3C(Cl)=C(Br)C=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
X-Gluc cyclohexanamine
Cat. No.:
HY-15935B
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