VF 488 Caspase 3 Assay Kit for Live Cells 

Cysteine-dependent aspartate-directed proteases (Caspases) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation.

Caspase 3, a cysteine protease also known as CPP32, Yama or apopain, belongs to the CED-3 subfamily. Caspase 3 can cleave procaspase 2, 6, 7, and 9, and can directly and specifically cleave many caspase substrates, including PARP, ICAD, gelsolin, and fodrin. In addition, Caspase 3 also plays a key role in the process of cell apoptosis, including chromatin condensation and DNA fragmentation.

MCE VF 488 Caspase 3 Assay Kit for Live Cells uses a fluorescent substrate with cell membrane permeability to detect Caspase 3 activity in live cells. The principle is that the substrate peptide DEVD coupled to a fluorogenic DNA dye is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase3 cleaves the substrate, releasing DNA dye, which migrates to the cell nucleus and stains DNA with bright green fluorescence, allowing real-time detection of apoptosis in living cells.

Features of MCE VF 488 Caspase 3 Assay Kit for Live Cells

1. Bifunctional: It can be used to detect caspase-3 activity and observe the morphological changes in the nucleus during apoptosis.

2. Compatibility: It can be assayed by fluorescence microscopy, flow cytometry or Fluorence microplate reader. After detection, the sample can be fixed with paraformaldehyde and subsequently a variety of immunostaining such as immunofluorescence and immunohistochemistry can be performed for further analysis.

3. Simple and Fast: The reagents can be added directly to the cell culture medium and the assay can be completed in 15-30 min.

4℃, 3 years

Protect from light