1. MCE Kits
  2. Cell Biology
  3. Cell Analysis
  4. Cell Apoptosis and Necrosis
  5. VF 488 Caspase 3 Assay Kit for Live Cells

VF 488 Caspase 3 Assay Kit for Live Cells 

Cat. No.: HY-K1088
Manual SDS

MCE VF 488 Caspase 3 Assay Kit for Live Cells uses a fluorescent substrate with cell membrane permeability to detect Caspase 3 activity in live cells.

VF 488 Caspase 3 Assay Kit for Live Cells
Size Price Stock Quantity
25 T USD 120 In-stock
100 T USD 360 In-stock

* Please select Quantity before adding items.

  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

Cysteine-dependent aspartate-directed proteases (Caspases) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation.

Caspase 3, a cysteine protease also known as CPP32, Yama or apopain, belongs to the CED-3 subfamily. Caspase 3 can cleave procaspase 2, 6, 7, and 9, and can directly and specifically cleave many caspase substrates, including PARP, ICAD, gelsolin, and fodrin. In addition, Caspase 3 also plays a key role in the process of cell apoptosis, including chromatin condensation and DNA fragmentation.

MCE VF 488 Caspase 3 Assay Kit for Live Cells uses a fluorescent substrate with cell membrane permeability to detect Caspase 3 activity in live cells. The principle is that the substrate peptide DEVD coupled to a fluorogenic DNA dye is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase3 cleaves the substrate, releasing DNA dye, which migrates to the cell nucleus and stains DNA with bright green fluorescence, allowing real-time detection of apoptosis in living cells.

Features of MCE VF 488 Caspase 3 Assay Kit for Live Cells

Bifunctional: It can be used to detect caspase-3 activity and observe the morphological changes in the nucleus during apoptosis.

Compatibility: It can be assayed by fluorescence microscopy, flow cytometry or Fluorence microplate reader. After detection, the sample can be fixed with paraformaldehyde and subsequently a variety of immunostaining such as immunofluorescence and immunohistochemistry can be performed for further analysis.

Simple and Fast: The reagents can be added directly to the cell culture medium and the assay can be completed in 15-30 min.

Storage

4℃, 3 years

Protect from light

Components
Components HY-K1088-25T HY-K1088-100T
Substrate (0.2 mM VF 488 Caspase 3, DMSO) 125 μL 500 μL
Inhibitor (2 mM Ac-DEVD-CHO, DMSO) 20 μL 100 μL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
VF 488 Caspase 3 Assay Kit for Live Cells
Cat. No.:
HY-K1088
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