Human Brain (Differentiation) Organoid Kit
Based on 1 Customer Validation
MCE Human Brain (Differentiation) Organoid Kit includes a basic culture medium and differentiation supplements, designed for the induction and culture of human brain organoids (hFBs).
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Storage :
HY-K6142-A: 4°C, 1 year. Shipping with blue ice.
HY-K6142-B: -20°C, 1 year. Avoid repeated freeze/thaw cycles. Shipping with dry ice.
- Manual (2088KB)
- COA
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
MCE Human Brain (Differentiation) Organoid Kit includes a basic culture medium and differentiation supplements, designed for the induction and culture of human brain organoids (hFBs).
During the expansion phase, hFBs are primarily composed of neural stem cells characterized by the expression of stem cell markers such as Sox2. Upon switching from the expansion medium to the differentiation medium, hFBs undergo differentiation into mature neuronal cells, exhibiting the expression of lineage-specific markers including Gfap, Map2, Cux1, and Dcx.
Protocol
Preparation of Human Brain Organoid Differentiation Medium
Prepare the differentiation medium according to the table below, mix thoroughly, and keep on ice. Perform all procedures under sterile conditions.
| Reagent | 10 mL | 20 mL | 50 mL | Final concentration |
|---|---|---|---|---|
| Human Brain Organoid Differentiation Basal Medium A | 9.5 mL | 19 mL | 47.5 mL | 1× |
| Human Brain Organoid Differentiation Culture Supplement B (25×) | 400 μL | 800 μL | 2 mL | 1× |
| Human Brain Organoid Differentiation Culture Supplement C (100×) | 100 μL | 200 μL | 500 μL | 1× |
Differentiation of Human Brain Organoids
1. Initial expansion culture: Culture the organoids with the corresponding Human Brain Organoid (Expansion) Kit (MCE Cat. No.: HY-K6141) for at least 2-5 d to support healthy growth and tissue integrity.
2. Medium pre-warming: Pre-warm the differentiation medium to 37°C before use.
3. Organoid washing: Carefully remove the expansion medium and wash the organoids once with Basal Medium A.
4. Distribution and medium addition: Transfer 4-8 organoids into each well of a 6-well plate and carefully add pre-warmed differentiation medium. Avoid bubbles and excessive mechanical stress.
5. Differentiation culture: Continue culture for 7-9 d and completely replace the medium with fresh pre-warmed differentiation medium every other day.
6. Completion: At the end of the differentiation period, the organoids display neuronal morphology and differentiation characteristics and are ready for downstream neuron-marker analysis.
Storage
HY-K6142-A: 4°C, 1 year. Shipping with blue ice.
HY-K6142-B: -20°C, 1 year. Avoid repeated freeze/thaw cycles. Shipping with dry ice.
Attention
1. This product is for R&D use only, not for drug, household, or other uses.
2. For your safety and health, please wear a lab coat and disposable gloves to operate.
Components
| Cat. No. | Product List | Components | HY-K6142 -100 mL |
HY-K6142 -500 mL |
Storage |
|---|---|---|---|---|---|
| HY-K6142-A | Human Brain Organoid Differentiation Basal Medium |
Basal Medium A | 100 mL | 500 mL | 4°C, 1 year. |
| HY-K6142-B | Human Brain Organoid Differentiation Culture Supplement |
Supplement B (25×) |
4 mL | 10 mL × 2 | -20°C, 1 year. Avoid repeated freeze/thaw cycles. |
| Supplement C (100×) |
1 mL | 5 mL |
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