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N-2 Serum-free Supplement (100×)

Customer Review

Based on 1 Customer Validation

MCE N-2 Serum-free Supplement (100×) can be used in conjunction with growth factors such as bFGF and EGF in serum-free neuronal cell culture mediums, DMEM and the DMEM/F12. This product is formulated with basal culture medium. The 5 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

  • Storage :
    -20℃,2 years. Keep away from light. Shipping with dry ice.
  • Experimental Validation Results for N-2 Serum-free Supplement (100×)
  • Experimental Validation Results for N-2 Serum-free Supplement (100×)
  • N-2 Serum-free Supplement (100×) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Description & Advantages

N-2 Supplement promotes cell growth and survival. It has been associated with the growth and expression of neuroblastoma, and with post-mitotic neuronal survival and expression in primary cultures of both the peripheral nervous system (PNS) and central nervous system (CNS). It is primarily used in the cultivation of neural cell lines and in the culture and directed differentiation of pluripotent stem cells. It can be used in conjunction with growth factors such as bFGF and EGF in serum-free neuronal cell culture mediums, DMEM and the DMEM/F12.

MCE N-2 Serum-free Supplement (100×) is an upgraded version of Bottenstein’s N-1 formulation and is a serum-free additive with clearly defined chemical components. It contains transferrin, recombinant insulin, progesterone, putrescine, and selenite, among others. The recommended working concentration in the cell culture medium is 1×.

Protocol

Cell Culture Supplementation

1. Completely thaw N-2 Serum-free Supplement (100×) and mix gently before use.

2. Under sterile conditions, add the supplement to sterile cell culture medium at 1 : 100 (v/v) to obtain a final working concentration of 1×.

3. Mix thoroughly and use directly for the appropriate cell culture.

Storage

-20℃,2 years.

Keep away from light.

Shipping with dry ice.

Components

Components HY-K3012A-5 mL HY-K3012A-10 mL HY-K3012A-50 mL
N-2 Serum-free Supplement (100×) 5 mL 2 × 5 mL 10 × 5 mL

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100 mg

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  • Experimental Validation Results for N-2 Serum-free Supplement (100×)
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    MedChemExpress Validation
    Cell line: B104 cells (rat neuroblastoma cells)
    Culture conditions: 37℃, 5% CO2 incubator
    Initial inoculation cell volume: 2 × 104/well (48-well plate)
    Culture medium: DMEM + 0.5% FBS + N2 (50×) + 1% PS
    Culture time: 5-6 Days
    The results showed that N-2 Serum-free Supplement (100×) (HY-K3012A ) can significantly promote the growth of B104 cells, with no significant difference compared with Competitor G.

  • Experimental Validation Results for N-2 Serum-free Supplement (100×)
    1/16

    MedChemExpress Validation
    Cell line: SD rat cortical astrocyte primary cells
    Culture conditions: 37℃, 5% CO2 incubator
    Initial inoculation cell amount: 3 × 104/well (48-well plate)
    Culture medium: DMEM + 0.5% FBS + N2 (50×) + 1% PS + EGF (2 ng/mL)
    Culture time: 7 Days
    The results showed that N-2 Serum-free Supplement (100×) (HY-K3012A) can significantly promote the growth of SD rat cortical astrocyte primary cells, and has certain advantages over Competitor G.

  • N-2 Serum-free Supplement (100×) Product Image
    1/16
  • Experimental Validation Results for N-2 Serum-free Supplement (100×)
  • Experimental Validation Results for N-2 Serum-free Supplement (100×)
  • N-2 Serum-free Supplement (100×) Product Image