1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite

Acetylcysteine (Synonyms: N-Acetyl-L-cysteine; LNAC; NAC)

Cat. No.: HY-B0215 Purity: >98.0%
Handling Instructions

Acetylcysteine is used mainly as a mucolytic, which protects against acetaminophen overdose-induced hepatotoxicity by maintaining or restoring hepatic concentrations of glutathione.

For research use only. We do not sell to patients.

Acetylcysteine Chemical Structure

Acetylcysteine Chemical Structure

CAS No. : 616-91-1

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
5 g USD 60 In-stock
Estimated Time of Arrival: December 31
10 g USD 72 In-stock
Estimated Time of Arrival: December 31
50 g   Get quote  

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    Acetylcysteine purchased from MCE. Usage Cited in: Sci Rep. 2016 Oct 27;6:35995.

    Pretreatment of NAC, a free radical scavenger, largely decreases the expression of caspase-3 and almost abolishes the H2O2 induced upregulation of PRX1. Western blot analysis shows the pretreatment of osteoblast cells with E2 partially abrogates the increased PRX1 and caspase-3 expressions induced by H2O2.

    Acetylcysteine purchased from MCE. Usage Cited in: Fundam Clin Pharmacol. 2017 Feb;31(1):64-74.

    Advanced oxidation production product (AOPP)-triggered apoptosis-related signals are triggered by ROS production. Human chondrocytes are pretreated with N-Acetylcysteine (NAC; 5 mM) for 60 min, and then, chondrocyte are co-incubated with AOPP and NAC for 24 h. (a) Pretreatment of ROS scavenger NAC markedly alleviates AOPP-triggered mitochondria-mediated apoptosis proteins. (b) Pretreatment of chondrocyte with NAC abrogates the intracellular Ca2+ mobilization. (c) NAC pretreatment atte

    Acetylcysteine purchased from MCE. Usage Cited in: Cell Death Discov. 2018 Apr 27;4:47.

    Wild-type, Prx1KO, and Prx1OE cells are each divided into three groups, which are control groups, groups treated with 100 ng/mL LPS, and groups treated with 100 ng/mL LPS and NAC. Western blot analysis is then performed with antibodies against Bax, Bcl-2, Cyto-c, caspase 3, and GAPDH, and band intensity is measured with densitometry.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Acetylcysteine is used mainly as a mucolytic, which protects against acetaminophen overdose-induced hepatotoxicity by maintaining or restoring hepatic concentrations of glutathione.

    In Vitro

    N-acetylcysteine prevents apoptotic DNA fragmentation and maintains long-term survival in the absence of other trophic support in serum-deprived PC12 cells. N-acetylcysteine also prevents death of PC12 cells and sympathetic neurons[2]. N-acetylcysteine causes dose-dependent reductions in viability in rat and human aortic smooth muscle cells[3]. N-acetylcysteine activates the Ras-extracellular signal-regulated kinase (ERK) pathway in PC12 cells. N-acetylcysteine protects neuronal cells from death evoked by withdrawal of trophic support. N-acetylcysteine increases nitric oxide (NO) release from protein-bound stores in vascular tissue. N-acetylcysteine pretreatment of PC12 cells interferes with NGF-dependent signaling and neurite outgrowth, and it is suggested that N-acetylcysteine interferes with redox-sensitive steps in the NGF mechanism[4].

    In Vivo

    N-acetylcysteine (150, 300 mg/kg) treatment significantly reduces liver transaminases in all groups of treatment, mostly in group N-acetylcysteine 300. Lung glutathione peroxidase is significantly increases in group N-acetylcysteine 300 (P=0.04), while the other oxidation biomarkers show no significant differences[1]. N-acetylcysteine improves cognition of 12-month-old SAMP8 mice in both the T-maze footshock avoidance paradigm and the lever press appetitive task without inducing non-specific effects on motor activity, motivation to avoid shock, or body weight[5].

    Clinical Trial
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 130 mg/mL (796.62 mM)

    : 50 mg/mL (306.39 mM; Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.1278 mL 30.6391 mL 61.2783 mL
    5 mM 1.2256 mL 6.1278 mL 12.2557 mL
    10 mM 0.6128 mL 3.0639 mL 6.1278 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.17 mg/mL (13.30 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.17 mg/mL (13.30 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.17 mg/mL (13.30 mM); Clear solution

    References
    Cell Assay
    [2]

    For survival experiments, washed cells are resuspended in RPM1 1640 medium and plated in 0.5 mL at a density of 8-10×105 per well in 24 well plastic culture dishes coated with rat tail collagen. To feed, but to avoid loss of floating cells, fresh medium (0.2 mL) is added to the cultures on days 1, 5, and 10. For experiments involving "primed" PC12 cells, cultures are pretreated for l-2 weeks with NGF in RPM1 1640 medium supplemented with 1% heat-iN-acetylcysteinetivated horse serum. The cells are then washed and passaged into serum-free RPM1 1640 medium.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [1]

    Rats: Rats are randomLy allocated into five groups: sham group (n=5), control group with IIR (n=8) and three groups with IIR who are given N-acetylcysteine in different dosages: 150 mg/kg intraperitoneally 5 min before ischemia (n=8, group N-acetylcysteine 150), 300 mg/kg i.p 5 min before ischemia (n=7, group N-acetylcysteine 300), and 150 mg/kg i.p 5 min before ischemia plus 150 mg/kg 5 min before reperfusion (n=7, group N-acetylcysteine 150 + 150). After 4 h of reperfusion, the animals are euthanized by exsanguination from the abdominal aorta.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    163.19

    Formula

    C₅H₉NO₃S

    CAS No.

    616-91-1

    SMILES

    SC[[email protected]@H](C(O)=O)NC(C)=O

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: >98.0%

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    Product Name:
    Acetylcysteine
    Cat. No.:
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    Acetylcysteine

    Cat. No.: HY-B0215