2. Academic Validation
  3. Eugenol Alleviates TGEV-Induced Intestinal Injury via Suppressing ROS/NLRP3/GSDMD-Dependent Pyroptosis

Eugenol Alleviates TGEV-Induced Intestinal Injury via Suppressing ROS/NLRP3/GSDMD-Dependent Pyroptosis

  • J Agric Food Chem. 2023 Jan 15. doi: 10.1021/acs.jafc.2c05833.
Kang Wang 1 2,  Daiwen Chen 1 2,  Bing Yu 1 2,  Jun He 1 2,  Xiangbing Mao 1 2,  Zhiqing Huang 1 2,  Hui Yan 1 2,  Aimin Wu 1 2,  Yuheng Luo 1 2,  Ping Zheng 1 2,  Jie Yu 1 2,  Junqiu Luo 1 2
Affiliations

Affiliations

  • 1 Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan Province 611130, People's Republic of China.
  • 2 Key Laboratory of Animal Disease-resistant Nutrition, Chengdu, Sichuan Province 611130, People's Republic of China.
PMID: 36642968 DOI: 10.1021/acs.jafc.2c05833
Abstract

Transmissible gastroenteritis virus (TGEV), a coronavirus, is one of the main causative agents of diarrhea in piglets and significantly impacts the global swine industry. Pyroptosis is involved in the pathogenesis of coronavirus, but its role in TGEV-induced intestinal injury has yet to be fully elucidated. Eugenol, an essential plant oil, plays a vital role in Antiviral innate immune responses. We demonstrate the preventive effect of eugenol on TGEV Infection. Eugenol alleviates TGEV-induced intestinal epithelial cell Pyroptosis and reduces intestinal injury in TGEV-infected piglets. Mechanistically, eugenol reduces the activation of NLRP3 inflammasome, thereby inhibiting TGEV-induced intestinal epithelial cell Pyroptosis. In addition, eugenol scavenges TGEV-induced Reactive Oxygen Species (ROS) increase, which in turn prevents TGEV-induced NLRP3 inflammasome activation and Pyroptosis. Overall, eugenol protects the intestine by reducing TGEV-induced Pyroptosis through inhibition of NLRP3 inflammasome activation, which may be mediated through intracellular ROS levels. These findings propose that eugenol may be an effective strategy to prevent TGEV Infection.

Keywords

NLRP3 inflammasome; coronavirus; eugenol; inflammation; pyroptosis; reactive oxygen species; transmissible gastroenteritis virus.

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