Molecular Biology

Molecular Biology enables scientists to research the molecular basis of biological activity. From PCR & qPCR & RT-PCR, vector construction, to nucleic acid gel electrophoresis, MCE provides a series of high-quality products to drive your research forward.

Nucleic Acid Gel Electrophoresis (15)

Nucleic Acid Gel Stain (2)

Cat. No.: HY-K1004 SYBR Green I Nucleic Acid Gel Stain

MCE SYBR Green I Nucleic Acid Gel Stain is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels.

Cat. No.: HY-K1007 Red Nucleic Acid Gel Stain (10,000×)

MCE Red Nucleic Acid Gel Stain (10,000×) is a nucleic acid stain that can be used as a safer alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acids in agarose gels or polyacrylamide gels.

Agarose (2)

Cat. No.: HY-K1029 Agarose With TAE Powder (1%)

MCE Agarose With TAE Powder (1%) mainly consists of proportionally mixed agarose and TAE Powder, and can be directly made into 1% agarose gel. The 20 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K1031 Agarose

MCE Agarose can be made into 0.5-2.5% agarose gel according to different needs and can resolve DNA and RNA fragments from 50-15,000 bp.

Loading & Running Buffer (3)

Cat. No.: HY-K1015 TAE Powder (1 L of 1×)

MCE TAE Powder (1 L of 1×) consists of Tris-acetate and EDTA-2Na, and is widely used in agarose gel electrophoresis of nucleic acid. The 20 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K1016 TBE Powder (1 L of 1×)

MCE TBE Powder (1 L of 1×) consists of Tris, Boric Acid and EDTA, and is widely used in agarose gel electrophoresis of nucleic acid. The 10 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K1032 6× DNA Loading Buffer with SDS

MCE 6× DNA Loading Buffer with SDS is a modified 6-fold buffer and mainly consists of glycerin, EDTA, SDS, Orange G and Xylene Cyanol FF. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

DNA Marker (8)

Cat. No.: HY-K0801 50 bp DNA Marker

The 50 bp DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis. The 250 μL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0802 100 bp DNA Marker

The 100 bp DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis. The 250 μL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0803 100 bp plus DNA Marker

The 100 bp plus DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis. The 250 μL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0804 500 bp DNA Marker

The 500 bp DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis. The 250 μL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0805 1 kb DNA Marker

The 1 kb DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis. The 250 μL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0806 2 kb DNA Marker

The 2 kb DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis. The 250 μL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0807 5 kb DNA Marker

The 5 kb DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis.The 250 μL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0808 15 kb DNA Marker

The 15 kb DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis.The 250 μL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Vector Construction (7)

Seamless Cloning (2)

Cat. No.: HY-K1041 Seamless DNA Assembly Plus Kit

MCE Seamless DNA Assembly Plus Kit contains an optimized mix of recombinase, reaction buffer, and additional cofactors that significantly improve the cloning efficiency and tolerance to impurities. This product can complete multiple DNA fragments recombination and takes only 5 minutes for single fragment, and the positive rate is more than 95%.

Cat. No.: HY-K1041A Seamless DNA Assembly Ultra Kit

MCE Seamless DNA Assembly Ultra Kit is a next-generation recombinant cloning kit that allows the recombination of single or multiple DNA fragments in a single reaction.

Ligase (2)

Cat. No.: HY-K1049 T4 DNA Ligase (Fast)

MCE T4 DNA Ligase (Fast) is produced by Escherichia coli carrying a T4 phage, catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.1 U=1 Weiss unit

Cat. No.: HY-KE8009 Taq DNA Ligase

This enzyme is a DNA ligase cloned from Thermus thermophilus HB8. It is expressed in E. coli using genetic recombination technology and purified and isolated multiple times.

Endonuclease (3)

Cat. No.: HY-KE8005 T7 Endonuclease I

T7 Endonuclease I can recognize and cleave incompletely paired DNA, cruciform structure DNA, Holliday structure or crossover DNA, heterologous double-stranded DNA, or cleave nicked double-stranded DNA at a slower rate.

Cat. No.: HY-KE8010 Exonuclease I

This product is obtained by expressing the Exo I gene (E. coli) in Escherichia coli and then purifying and isolating it multiple times.

Cat. No.: HY-KE8013 Protein Deglycosylation Kit (for N-Linked & Simple O-Linked Glycans)

Protein Deglycosylation Kit (for N-Linked & Simple O-Linked Glycans) includes the enzymes and reagents necessary for the removal of all N-linked and relatively simple O-linked glycans. The enzymatic reagents are a mixture of three glycosidases, namely PNGase F, O-Glycosidase, and α2-3,6,8,9 Neuraminidase. All enzymes are recombinant enzymes expressed in Escherichia coli BL21 and subsequently purified. The molecular weights of these enzymes are as follows: PNGase F is 36 kDa, O-Glycosidase is 147 kDa, and α2-3,6,8,9 Neuraminidase is 66 kDa.

Restriction Endonuclease (60)

Cat. No.: HY-KE7001 ApaL I

MCE ApaL I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Alw44 I, Vne I.

Cat. No.: HY-KE7006 Bgl II

MCE Bgl II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7007 Bsa I

MCE Bsa I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Eco31 I, Bso31 I, BspTN I.

Cat. No.: HY-KE7008 BstB I

MCE BstB I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Asu II, Bpu14 I, Bsp119 I, BspT104 I, Nsp V, Sfu I.

Cat. No.: HY-KE7010 Cla I

MCE Cla I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: BspD I, Bsa29 I, BseC I, Bsu15 I, BsuTU I.

Cat. No.: HY-KE7009 BstE II

MCE BstE II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: BstP I, Eco91 I, EcoO65 I, PspE I.

Cat. No.: HY-KE7003 Asc I

MCE Asc I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: PalA I, Sgs I.

Cat. No.: HY-KE7002 Avr II

MCE Avr II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: AspA2 I, Bln I, XmaJ I.

Cat. No.: HY-KE7004 BamH I

MCE BamH I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7005 Bcl I

MCE Bcl I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Fba I, Ksp22 I.

Cat. No.: HY-KE7045 Xho I

Xho I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: PaeR7 I, Sfr274 I, Sla I.

Cat. No.: HY-KE7046 Nru I

Nru I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Bsp68 I, BtuM I, Rru I.

Cat. No.: HY-KE7047 Age I

Age I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: AsiG I, CspA I, BshT I, PinA I.

Cat. No.: HY-KE7048 BspQ I

BspQ I is one of the endonucleases of Type IIs that recognize non-palindromic sequences and cut outside of the recognition sequence, and is commonly used in Golden Gate assembly. Isoschizomers: Sap I, Lgu I, PciS I.

Cat. No.: HY-KE7049 Sge I

Sge I restriction enzyme recognizes and cleaves DNA targets containing 5-methylcytosine on one or both DNA strands, and cuts best at 37℃.

Cat. No.: HY-KE7050 BsmB I

BsmB I is one of the endonucleases of Type IIs that recognize non-palindromic sequences and cut outside of the recognition sequence, and is commonly used in Golden Gate assembly. Isoschizomers: Esp3 I, BstGZ53 I, Esp16 I, Esp23 I.

Cat. No.: HY-KE7052 BsmB I, ADCF

BsmB I, ADCF is animal derived component free, and has good redundancy of enzyme activity, which can easily cope with excessive substrate or difficult template enzyme cutting. Isoschizomers: Esp3 I, BstGZ53 I, Esp16 I, Esp23 I.

Cat. No.: HY-KE7053 EcoR I, ADCF

EcoR I, ADCF is animal derived component free, and has good redundancy of enzyme activity, which can easily cope with excessive substrate or difficult template enzyme cutting. Isoschizomers: Fun II.

Cat. No.: HY-KE7054 Xba I, ADCF

Xba I, ADCF is animal derived component free, and has good redundancy of enzyme activity, which can easily cope with excessive substrate or difficult template enzyme cutting.

Cat. No.: HY-KE7011 Dpn I

MCE Dpn I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Mal I.

Cat. No.: HY-KE7012 Dpn II

MCE Dpn II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Mbo I, Sau3A I, Bsp143 I, BstMB I, Nde II, BstKT I.

Cat. No.: HY-KE7013 Eag I

MCE Eag I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: BseX3 I, BstZ I, EclX I, Eco52 I.

Cat. No.: HY-KE7014 EcoR I

MCE EcoR I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Fun II.

Cat. No.: HY-KE7015 EcoR V

MCE EcoR V is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Eco32 I.

Cat. No.: HY-KE7016 Esp3 I

MCE Esp3 I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: BsmB I.

Cat. No.: HY-KE7017 Fsp I

MCE Fsp I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Acc16 I，Nsb I.

Cat. No.: HY-KE7018 Hind III

MCE Hind III is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7019 Hinf I

MCE Hinf I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7020 Hpa I

MCE Hpa I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: KspA I.

Cat. No.: HY-KE7024 Mnl I

MCE Mnl I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7025 Nco I

MCE Nco I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Bsp19 I.

Cat. No.: HY-KE7026 Nde I

MCE Nde I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: FauND I.

Cat. No.: HY-KE7027 Nhe I

MCE Nhe I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Bmt I.

Cat. No.: HY-KE7028 Not I

MCE Not I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: CciN I.

Cat. No.: HY-KE7022 Kpn I

MCE Kpn I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Asp718 I, Acc65 I.

Cat. No.: HY-KE7023 Mlu I

MCE Mlu I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7029 Nsi I

MCE Nsi I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: EcoT22 I, Mph1103 I, Zsp2 I.

Cat. No.: HY-KE7030 Pac I

MCE Pac I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7031 Pst I

MCE Pst I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: BspMA I.

Cat. No.: HY-KE7032 Pvu II

MCE Pvu II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7033 Sac I

MCE Sac I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Psp124B I, Sst I, Ecl136 II, EcoICR I.

Cat. No.: HY-KE7034 Sac II

MCE Sac II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Cfr42 I, Ksp I, Sfr303 I, SgrB I.

Cat. No.: HY-KE7035 Sal I

MCE Sal I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7036 Sbf I

MCE Sbf I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Sda I, Sse8387 I.

Cat. No.: HY-KE7037 Sfi I

MCE Sfi I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7038 Sma I

MCE Sma I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Cfr9 I, Xma I.

Cat. No.: HY-KE7039 Spe I

MCE Spe I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Ahl I, Bcu I.

Cat. No.: HY-KE7040 Sph I

MCE Sph I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Pae I.

Cat. No.: HY-KE7041 Ssp I

MCE Ssp I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7042 Stu I

MCE Stu I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Eco147 I, Pce I, SseB I.

Cat. No.: HY-KE7043 Taq I

Taq I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7044 Xba I

Xba I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Cat. No.: HY-KE7057 O-Glycosidase

O-Glycosidase is highly specific and can release Galβ1-3GalNAc from serine, threonine residues or as part of a glycopeptide or glycoprotein. Applied to glycoprotein biosynthesis analysis, O-glycan bioactive protein O-glycosylation detection and binding site analysis.

Cat. No.: HY-KE7056 RNase III

RNase III is a specific exonuclease expressed in E.coli that can cleave dsRNA into 12-15 bp dsRNA fragments.

Cat. No.: HY-KE7055 RNase R

RNase R is a magnesium ion-dependent 3'-5' exoribonuclease that can increase the abundance of circular RNA by degrading linear RNA in mixed RNA, thereby enriching circular RNA.

Cat. No.: HY-KE7058 RNase H

RNase H is an endoribonuclease expressed and purified from Escherichia coli and can specifically hydrolyze RNA in DNA-RNA hybrid strands.

Cat. No.: HY-KE7066 FnCas12a

FnCas12a(Cpf1), is an RNA-guided, DNA-editable recombinant endonuclease that can be used for gene editing and detection.

Cat. No.: HY-KE7067 Lambda DNA

Lambda DNA (λ DNA) is the DNA in λ phages and is used as a substrate for restriction endonucleases. This product is methylated Lambda DNA.

Cat. No.: HY-KE8012 Protein Deglycosylation Kit(for O-linked Glycans)

Protein Deglycosylation Kit (for O-linked Glycans)contains O-Glycosidase and a2-3,6,8,9 Neuraminidase.Both enzymes are crucial tool enzymes for glycosylation research and are used in combination to enzymatically release O-linked glycans from glycoproteins.O-Glycosidase is capable of cleaving and releasing Core 1 (Gal-B(1-3)-GalNAc-)and Core 3(GIcNAc-B(1-3)-GalNAc-)O-linked disaccharides,which are linked to the hydroxyl groups of Ser or Thr residues in glycoproteins.a2-3,6,8,9 Neuraminidase can remove a(2,3)-,a(2,6)-,a(2,8)-,and a(2,9)-sialic acid residues from the non-reducing termini of O-linked glycans.

Cat. No.: HY-KE8014 Protein Deglycosylation Kit (for N-linked & Complex O-linked Glycans)

Protein Deglycosylation Kit (for N-linked & Complex O-linked Glycans) includes the enzymes and reagents reuired for the removal of all N-linked and some complex O-linked glycans. The enzyme reagent in this kit is a mixture of five glycosidases, namely PNGase F, O-Glycosidase, α2-3,6,8,9 Neuraminidase, β1-4 Galactosidase, and β-N-Acetylhexosaminidase. All enzymes in this kit are recombinant enzymes expressed in Escherichia coli BL21 and subsequently purified. The molecular weights of these enzymes are as follows: PNGase F is 36 kDa, O-Glycosidase is 14 kDa,α2-3,6,8,9 Neuraminidase is 66 kDa, β1-4 Galactosidase is 94 kDa, and β-N-Acetylhexosaminidase is 55 kDa.

Materials (1)

Cat. No.: HY-E0001 Cooling Rack

MCE® Cooling Rack can be used to prevent enzymes from becoming inactive as well as for PCR sample preparation, sample transport, and to slow thawing of frozen samples throughout your entire lab workflow.

PCR & qPCR (23)

Polymerase Chain Reaction (PCR) (4)

Cat. No.: HY-K0531 2× PCR Master Mix (with Dye)

MCE 2× PCR Master Mix (with Dye) is provided as a simple-to-use, stabilized 2× formulation that includes all components for PCR except sample DNA, primers and water, in which bromophenol blue dye is included. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0532 2× Fast PCR Master Mix (with Dye)

MCE 2× Fast PCR Master Mix (with Dye) is provided as a simple-to-use, stabilized 2× formulation that includes all components for fast PCR except sample DNA, primers and water, in which bromophenol blue dye is included. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0533 2× High-Fidelity PCR Master Mix

MCE 2× High-Fidelity PCR Master Mix is provided as a simple-to-use, stabilized 2× formulation that includes all components for PCR except sample DNA, primers and water. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0533A 2× High-Fidelity PCR Master Mix (with Dye)

MCE 2× High-Fidelity PCR Master Mix (with Dye) is a new type of ready-to-use high-fidelity PCR amplification master mix.During the reaction, only template and primers need to be added to quickly and efficiently complete the PCR process, reducing the risk of contamination during operation. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Real-Time PCR (6)

Cat. No.: HY-K0501 SYBR Green qPCR Master Mix

MCE qPCR Master Mix is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water. The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0521 SYBR Green qPCR Master Mix (High ROX)

MCE SYBR Green qPCR Master Mix (High ROX) is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water, in which High ROX Reference Dye is included. The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0522 SYBR Green qPCR Master Mix (Low ROX)

MCE SYBR Green qPCR Master Mix (Low ROX) is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water, in which Low ROX Reference Dye is included. The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0523 SYBR Green qPCR Master Mix (No ROX)

MCE SYBR Green qPCR Master Mix (No ROX) is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water, in which no ROX Reference Dye is included. The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0501A SYBR Green qPCR Master Mix (Universal)

MCE SYBR Green qPCR Master Mix (Universal) is compatible with all qPCR instruments. 2× ready-to-use formulations which consist of antibody-mediated hot start polymerase, MgCl2, dNTPs and a proprietary reaction buffer, produce optimal results in qPCR experiments. The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0524 2-Color Tracking SYBR qPCR Master Mix (Universal)

MCE 2-Color Tracking SYBR qPCR Master Mix (Universal) is a 2× concentration premixed reagent utilizing a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR (qPCR). The 500 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

DNA Polymerase (6)

Cat. No.: HY-KE8001 Phi29 DNA Polymerase

Phi29 DNA Polymerase is a mesophilic DNA polymerase cloned from Bacillus subtilis phage Phi29.

Cat. No.: HY-KE8002 Bst DNA Polymerase, Full Length

Bst DNA Polymerase is derived from Bacillus stearothermophilus and has 5'→3' DNA polymerase activity and double-stranded specific 5'→3' exonuclease activity.

Cat. No.: HY-KE8003 Bst DNA Polymerase, Large Fragment

Bst DNA Polymerase large fragment is a part of Bacillus stearothermophilus DNA polymerase, which is derived from E. coli strain. It is expressed in E. coli and purified and isolated multiple times.

Cat. No.: HY-KE8007 DNA Polymerase I(E.coli)

This product is a DNA polymerase obtained by expressing DNA Polymerase I (E. coli) in E. coli and purifying and isolating it multiple times.

Cat. No.: HY-KE8008 T4 DNA Polymerase

T4 DNA polymerase has 5′→3′ DNA polymerase activity and can catalyze the synthesis of DNA along the 5′→3′ direction with the presence of templates and primers.

Cat. No.: HY-KE8011 Phi29 DNA Polymerase（mutant）

Phi29 DNA polymerase is a protein-engineered Phi29 DNA polymerase expressed in E. coli and then purified and isolated multiple times. Compared with wild-type Phi29 DNA polymerase, it has higher amplification efficiency and sensitivity, and can greatly shorten the reaction time.

Direct PCR (7)

Cat. No.: HY-K0536 Mouse Tissue Direct PCR Kit – Pro (with Dye)

MCE Mouse Tissue Direct PCR Kit – Pro (with Dye) is specifically designed for direct PCR amplification from tissue samples. It enables rapid, one-step release of genomic DNA from various mouse tissues (such as tail, ear, toe, and muscle), which can be directly used for downstream PCR amplification and analysis without the need for conventional DNA extraction and purification steps, thereby significantly simplifying the experimental workflow.

Cat. No.: HY-K0534 Mouse Tissue Direct PCR Kit (with Dye)

MCE Mouse Tissue Direct PCR Kit (with Dye) is specifically designed for direct PCR amplification from tissue samples. It enables rapid, one-step release of genomic DNA from various mouse tissues (such as tail, ear, toe, and muscle), which can be directly used for downstream PCR amplification and analysis without the need for conventional DNA extraction and purification steps, thereby significantly simplifying the experimental workflow.

Cat. No.: HY-K0535 Mouse Tissue Direct PCR Plus Kit

MCE Mouse Tissue Direct PCR Plus Kit is specifically designed for direct PCR amplification from tissue samples. It enables rapid, one-step release of genomic DNA from various mouse tissues (such as tail, ear, toe, and muscle), which can be directly used for downstream PCR amplification and analysis without the need for conventional DNA extraction and purification steps, thereby significantly simplifying the experimental workflow.

Cat. No.: HY-K0537 Blood Direct PCR Kit (with Dye)

MCE Blood Direct PCR Kit (with Dye) is specifically designed for direct amplification from blood samples. It enables PCR amplification directly from whole blood without the need for conventional DNA extraction and purification steps, thereby significantly simplifying the workflow and improving detection efficiency.

Cat. No.: HY-K0538 Animal Tissue Direct PCR Kit (with Dye)

MCE Animal Tissue Direct PCR Kit (with Dye) is specifically designed for direct amplification from a variety of animal tissue samples. It enables rapid release of genomic DNA from animal tissues (such as insect legs/wings, mouse tails, ears, toes, skin, and internal organs) in a single step, allowing the lysate to be used directly for downstream PCR amplification without the need for conventional DNA extraction and purification procedures, thereby significantly simplifying the workflow.

Cat. No.: HY-K0539 Plant Tissue Direct PCR Kit (with Dye)

MCE Plant Tissue Direct PCR Kit (with Dye) is specifically designed for direct amplification from various plant leaf samples. The kit enables rapid release of genomic DNA from plant tissues (such as rice, maize, tobacco, rapeseed, etc.) in a single step. The resulting lysate can be directly used for downstream PCR amplification without the need for conventional DNA extraction and purification, thereby significantly simplifying the experimental workflow.

Cat. No.: HY-K0005 Mouse Tissue Lysis Buffer

MCE Mouse Tissue Lysis Buffer formulated with a highly efficient lysis buffer system, enabling rapid disruption of various mouse tissue samples (e.g., tail, ear, toe, muscle) and efficient release of genomic DNA. The lysate can be directly used as a template in PCR reactions without the need for additional extraction or purification steps, ensuring a simple and streamlined workflow.

RT-PCR (7)

Reverse transcription PCR (6)

Cat. No.: HY-K0510A RT Master Mix for qPCR II

MCE RT Master Mix for qPCR II is a convenient, ready-to-use formulation for reverse transcription. With advanced reverse transcriptase, this kit can synthesize cDNA more rapidly, more specifically. The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0511A RT Master Mix for qPCR II (gDNA digester plus)

MCE RT Master Mix for qPCR II is a convenient, ready-to-use formulation for reverse transcription and can eliminate genomic DNA (gDNA) contaminations in RNA samples. With updated reverse transcriptase, this kit can synthesize cDNA more rapidly, more specifically. The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0511 RT Master Mix for qPCR (gDNA digester plus)

MCE RT Master Mix for qPCR (gDNA digester plus) is a convenient, ready-to-use formulation for reverse transcription and can eliminate genomic DNA (gDNA) contaminations in RNA samples. The cDNA product can be directly applied as a template in a standard PCR and real time quantitative PCR (qPCR). The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0512 microRNA RT Master Mix for qPCR Ⅲ (poly A)

MCE microRNA RT Master Mix for qPCR Ⅲ (poly A) uses poly(A)-tailing method to perform reverse transcription from miRNA first-strand to cDNA.

Cat. No.: HY-KE8004 Reverse Transcriptase

Reverse Transcriptase is a reverse transcriptase that clones and expresses the deletion mutant RNase H- of M-MuLV through genetic recombination technology.

Cat. No.: HY-K0513 RT Master Mix for qPCR III

MCE MCE Reverse Transcription Kit III is suitable for the conventional synthesis of cDNA from RNA and the stem-loop method for the synthesis of cDNA from microRNA (miRNA).

RNase Inhibitor (1)

Cat. No.: HY-K1033 RNase Inhibitor

RNase Inhibitor is a 50 kDa recombinant protein expressed in E.coli, and can specifically inhibit RNase A, B and C. The 10 KU volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Sequencing (1)

Enzymes (1)

Cat. No.: HY-KE8006 Terminal Deoxynucleotidyl Transferase (TdT)

Terminal Deoxynucleotidyl Transferase (TdT) is a template-independent DNA polymerase that catalyzes the binding of deoxynucleotides to the 3' hydroxyl end of oligonucleotides, single-stranded DNA, or double-stranded DNA.