Mycoplasma Detection Plus Kit (PCR) 

Mycoplasma are a class of prokaryotic microorganisms characterized by extremely small size, simple structure, and the absence of a cell wall, and they represent one of the most common and harmful sources of contamination in cell culture systems. Mycoplasma contamination is highly insidious; even at concentrations as high as 10⁸ organisms/mL, no obvious changes in medium turbidity or pH are usually observed, making it difficult to detect by visual inspection or routine observation of culture conditions. Mycoplasma infection exerts significant cytotoxic effects on cultured cells and can induce a series of adverse changes, including reduced proliferation rate, abnormal morphology, chromosomal aberrations, and metabolic disorders. In severe cases, it may lead to abnormal differentiation or even cell death, thereby markedly interfering with normal cellular functions. Mycoplasma contamination not only seriously compromises the reliability, reproducibility, and consistency of experimental data, but may also directly result in experimental failure. Therefore, timely and accurate detection of mycoplasma contamination is essential to ensure cell culture quality and experimental validity.

MCE Mycoplasma Detection Plus Kit (PCR) employs polymerase chain reaction (PCR) technology for rapid and sensitive detection of mycoplasma contamination in cultured cells and related biological samples. The kit contains a PCR premix and mycoplasma-specific primers designed against conserved regions of the 16S rRNA gene. Cell culture supernatant or cell lysates can be used directly as templates for specific amplification of mycoplasma DNA, enabling rapid identification of mycoplasma contamination.

Features of MCE Mycoplasma Detection Plus Kit (PCR):

1. High sensitivity: capable of detecting as few as 20 copies of mycoplasma DNA, meeting the requirements for low-level contamination detection.

2. High specificity: the PCR system specifically amplifies mycoplasma DNA without amplifying eukaryotic or common bacterial genomic DNA, effectively minimizing non-specific interference.

3. User-friendly operation: no genomic DNA extraction is required, making it suitable for high-throughput screening of large numbers of cell samples; no heat pretreatment is required for samples such as cell culture supernatants or animal sera.

4. Rapid detection: the entire detection procedure can be completed within approximately 1 h, significantly improving efficiency.

5. Reliable results: the kit is supplied with both positive and negative controls to facilitate quality control and result interpretation, ensuring accuracy and reproducibility.

-20°C, 2 years

Shipping with dry ice.

1. Before the experiment, ensure that all components of the kit are fully thawed and mixed well. After use, store at -20°C.

2. The serum and antibiotics in the cell culture medium do not affect the experimental results.

3. It is recommended to set up a positive control and a negative control for each experiment to ensure the accuracy of the results.

4. To avoid cross-contamination during experimental operations, it is advisable to perform them in a standard PCR area.

5. Since mycoplasmas are present in the oral cavity, wear a mask during operations to prevent sample contamination and false positive results.

6. To ensure the reliability and stability of the experiment, it is recommended to regularly check for mycoplasma contamination in cells and cell culture reagents.

7. This product is for R&D use only, not for drug, household, or other uses.

8. For your safety and health, please wear a lab coat and disposable gloves to operate.