Polyamine Supplement (1000×)
Based on 1 Customer Validation
MCE Polyamine Supplement (1000×) can be used as a component of the CEPT Cocktail in combination with Emricasan, Chroman 1, and trans-ISRIB to enhance the survival of pluripotent stem cells (PSCs) and promote single-cell clonal expansion. In addition, this supplement improves the viability of PSCs and their differentiated derivatives during cryopreservation, organoid culture, single-cell cloning, and genome editing, thereby enhancing the robustness and reproducibility of downstream applications.
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Storage :
According to the experimental requirements, add Polyamine Supplement (1000×) to sterile cell culture medium at a 1:1000 (v/v) dilution ratio and mix thoroughly before use. For example, add 1 mL of Polyamine Supplement (1000×) to 1 L of cell culture medium.
- Manual (945KB)
- COA
Description & Advantages
MCE Polyamine Supplement (1000×) is a cell culture medium supplement composed of putrescine, spermine, and spermidine.
MCE Polyamine Supplement (1000×) can be used as a component of the CEPT Cocktail in combination with Emricasan, Chroman 1, and trans-ISRIB to enhance the survival of pluripotent stem cells (PSCs) and promote single-cell clonal expansion. In addition, this supplement improves the viability of PSCs and their differentiated derivatives during cryopreservation, organoid culture, single-cell cloning, and genome editing, thereby enhancing the robustness and reproducibility of downstream applications.
Protocol
-20°C, 2 years
Storage
According to the experimental requirements, add Polyamine Supplement (1000×) to sterile cell culture medium at a 1:1000 (v/v) dilution ratio and mix thoroughly before use. For example, add 1 mL of Polyamine Supplement (1000×) to 1 L of cell culture medium.
Attention
1. This product has been sterile-filtered. Pay attention to aseptic operation to avoid contamination.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.