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Protein ubiquitination is a refined post-translational modification ubiquitous in all eukaryotes that is required for many cellular processes, including protein degradation by the proteasome, cell cycle progression, transcriptional regulation, DNA repair and signal transduction. Ubiquitination is catalyzed by ubiquitin-activating (E1), ubiquitin-conjugating (E2) and ubiquitin ligase (E3) enzymes. E3s mediate the transfer of ubiquitin from an E2 enzyme to specific substrate proteins at the end of a three-enzyme cascade.
E3s are the most heterogeneous class of enzymes in the ubiquitination pathway, as they mediate substrate specificity. They can be classified into two groups: 1) the single-subunit group, including several subfamilies based upon their mechanisms of action and the presence of specific domains: HECT (Homology to E6-AP C Terminus), RING (Really Interesting New Gene) and U-box type E3s; 2) the multi-subunit group, Cullin-RING box1-Ligases (CRLs), are further divided into four subfamilies: SCF (S phase kinase-associated protein 1–Cullin 1–F-box), BTB (Bric-a-brac–Tramtrack–Broad complex), DDB (DNA Damage-Binding domain-containing) and APC (anaphase-promoting complex). To date, >650 E3 ubiquitin ligases have been described in humans.
XIAP protein is a multifunctional regulator that controls apoptosis, inflammation, immunity, copper homeostasis, and various signaling pathways. As a caspase inhibitor, it blocks CASP3 and CASP7 substrate access, keeping CASP9 inactive. XIAP Protein, Human (Avi) is the recombinant human-derived XIAP protein, expressed by E. coli , with C-Avi labeled tag. The total length of XIAP Protein, Human (Avi) is 236 a.a., with molecular weight of ~29.1 kDa.
XIAP protein is a multifunctional regulator that controls apoptosis, inflammation, immunity, copper homeostasis, and various signaling pathways. As a caspase inhibitor, it blocks CASP3 and CASP7 substrate access, keeping CASP9 inactive. XIAP Protein, Human (His) is the recombinant human-derived XIAP protein, expressed by E. coli , with N-His labeled tag. The total length of XIAP Protein, Human (His) is 105 a.a., with molecular weight of ~14.8 kDa.
Tripartite motif-containing protein 5; RING finger protein 88; TRIM5; RNF88
Human
E. coli
The TRIM5 protein is a capsid-specific restriction factor that prevents non-host adapted retroviral infection. TRIM5 acts as a pattern recognition receptor that activates innate immune signaling by triggering E3 ubiquitin ligase activity upon binding to viral capsids. TRIM5 restricts various retroviruses and regulates autophagy. TRIM5 Protein, Human (His) is the recombinant human-derived TRIM5 protein, expressed by E. coli , with N-6*His labeled tag. The total length of TRIM5 Protein, Human (His) is 248 a.a., with molecular weight of ~33.0 kDa.
The ITCH/AIP4 protein, a member of the Nedd4 family of ubiquitin ligases, transfers ubiquitin to protein substrates for lysosomal degradation. It regulates cell differentiation and immune responses and is associated with autoimmune disease. Alternative splicing produces multiple isoforms. ITCH/AIP4 is expressed ubiquitously, with notable levels in the testis, esophagus, and 25 other tissues. ITCH/AIP4 Protein, Human (solution) is the recombinant human-derived ITCH/AIP4 protein, expressed by E. coli , with tag free. and with residual Gly-Pro in N-terminal (not related to functional changes) mutation. The total length of ITCH/AIP4 Protein, Human (solution) is 378 a.a., with molecular weight of ~40 kDa.
E3 ubiquitin-protein ligase RNF43; RING finger protein 43; RNF43
Human
HEK293
RNF43 protein is encoded by the RNF43 gene and is a RING-type E3 ubiquitin ligase. It negatively regulates Wnt signaling by increasing ubiquitination of Frizzled receptors, resulting in reduced surface levels of these receptors. RNF43 Protein, Human (HEK293, His) is the recombinant human-derived RNF43 protein, expressed by HEK293 , with C-His labeled tag. The total length of RNF43 Protein, Human (HEK293, His) is 174 a.a., with molecular weight of ~25-38 kDa.
NEDD4-like E3 ubiquitin-protein ligase WWP2; Atrophin-1-interacting protein 2; AIP2; WWP2
Human
Sf9 insect cells
The WWP2 protein is an E3 ubiquitin protein ligase that coordinates multiple ubiquitination events, including “Lys-63”-linked polyubiquitination of POU5F1, targeting it for proteasomal degradation in embryonic stem cells (ESCs). WWP2 also ubiquitinates EGR2, inhibits activation-induced cell death in T cells, and targets SLC11A2, which is enhanced by NDFIP1 and NDFIP2. WWP2 Protein, Human (sf9, His-GST) is the recombinant human-derived WWP2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of WWP2 Protein, Human (sf9, His-GST) is 870 a.a., with molecular weight of ~120 kDa.
E3 ubiquitin-protein ligase RNF43; RING finger protein 43; RING-type E3 ubiquitin transferase RNF43; Rnf43
Mouse
HEK293
RNF43 Protein, a receptor tyrosine kinase, regulates the Wnt signaling pathway by promoting the degradation of Frizzled receptors. It plays a crucial role in embryonic development and tissue homeostasis. Dysregulation of RNF43 Protein is associated with various cancers, making it a potential therapeutic target in cancer treatment. RNF43 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived RNF43 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of RNF43 Protein, Mouse (HEK293, Fc) is 174 a.a., with molecular weight of 53-60 kDa.
RNF114; E3 ubiquitin-protein ligase RNF114; RING finger protein 114; RING-type E3 ubiquitin transferase RNF114; Zinc finger protein 228; Zinc finger protein 313
Human
E. coli
The RNF114 protein is an E3 ubiquitin protein ligase that critically regulates a variety of cellular processes by ubiquitinating various substrates. RNF114 is involved in cell cycle progression, apoptosis, osteoclastogenesis and immunity, serving as a negative regulator of NF-kappa-B-dependent transcription, affecting immune responses by ubiquitinating TNFAIP3 and potentially TRAF6. RNF114 Protein, Human is the recombinant human-derived RNF114 protein, expressed by E. coli , with tag free. The total length of RNF114 Protein, Human is 227 a.a., .
MDM2 protein is a key E3 ubiquitin protein ligase that coordinates cellular processes by ubiquitinating and degrading p53/TP53 and inhibiting its tumor suppressor function. In addition to p53/TP53 regulation, MDM2 inhibits p53/TP53- and p73/TP73-mediated responses, self-ubiquitinates, and targets ARRB1 for degradation. MDM2 Protein, Human is the recombinant human-derived MDM2 protein, expressed by E. coli , with tag free. The total length of MDM2 Protein, Human is 95 a.a., with molecular weight of 11.1 kDa.
Tripartite motif-containing protein 5; RING finger protein 88; TRIM5; RNF88
Human
E. coli
The TRIM5 protein is a capsid-specific restriction factor that prevents non-host adapted retroviral infection. TRIM5 acts as a pattern recognition receptor that activates innate immune signaling by triggering E3 ubiquitin ligase activity upon binding to viral capsids. TRIM5 restricts various retroviruses and regulates autophagy. TRIM5 Protein, Human (N-His) is the recombinant human-derived TRIM5 protein, expressed by E. coli , with N-6*His labeled tag. The total length of TRIM5 Protein, Human (N-His) is 248 a.a., with molecular weight of ~30 kDa.
ITCH/AIP4 Protein, an E3 ubiquitin-protein ligase, catalyzes 'Lys-29,' 'Lys-48,' and 'Lys-63'-linked ubiquitin conjugation, influencing inflammatory signaling pathways. It forms a ubiquitin-editing complex, promoting RIPK1 degradation and termination of TNF- or LPS-mediated NFKB1 activation. It regulates transcription factors, immune response, and hematopoietic lineage development. ITCH/AIP4 targets JUN, JUNB, DTX1, and MAVS for degradation, modulates Wnt receptor sorting, and controls CXCR4 trafficking. It also regulates apoptosis, reactive oxygen species levels, and inhibits influenza A virus replication. ITCH/AIP4 Protein, Human is the recombinant human-derived ITCH/AIP4 protein, expressed by E. coli , with tag free. The total length of ITCH/AIP4 Protein, Human is 378 a.a., with molecular weight of ~45 kDa.
Kelch-like protein 2; Actin-binding protein Mayven; KLHL2
Human
E. coli
KLHL2 is a substrate-specific linker in the BCR E3 ubiquitin ligase complex that directs ubiquitination and proteasomal degradation of targets such as NPTXR, WNK1, WNK3, and WNK4. KLHL2 is critical for cellular homeostasis and catalyzes NPTXR ubiquitination, emphasizing its role in targeted protein turnover. KLHL2 Protein, Human (His) is the recombinant human-derived KLHL2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of KLHL2 Protein, Human (His) is 306 a.a., with molecular weight of ~63 KDa.
The HACE1 protein is an E3 ubiquitin protein ligase that regulates Golgi membrane fusion and small GTPases. HACE1 Protein, Human (His) is the recombinant human-derived HACE1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of HACE1 Protein, Human (His) is 908 a.a., .
E3 ubiquitin-protein ligase RNF43; RING finger protein 43; RNF43
Human
HEK293
RNF43 is an E3 ubiquitin protein ligase and a key negative regulator in the Wnt signaling pathway. Its role involves mediating the ubiquitination, endocytosis, and subsequent degradation of components within the Wnt receptor complex, particularly Frizzled. RNF43 Protein, Human (HEK293, Fc) is the recombinant human-derived RNF43 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of RNF43 Protein, Human (HEK293, Fc) is 174 a.a., with molecular weight of ~52 kDa.
CBLC Protein, an E3 ubiquitin-protein ligase, crucially regulates the ubiquitin-proteasome system by accepting ubiquitin from specific E2 enzymes and promoting substrate degradation. It forms a functional complex with UB2D1, UB2D2, and UB2D3. In EGFR-mediated signaling, CBLC ubiquitinates EGFR upon EGF activation, leading to its degradation. Isoform 1 inhibits EGF-stimulated MAPK1 activation, while CBLC, along with CD2AP, regulates RET degradation, transforming from an inhibitor to a promoter, limiting GDNF's potency on neuronal survival during Ret signaling. CBLC Protein, Human (sf9, His-GST) is the recombinant human-derived CBLC protein, expressed by Sf9 insect cells, with N-His, N-GST labeled tag. The total length of CBLC Protein, Human (sf9, His-GST) is 474 a.a., with molecular weight of ~66 kDa.
Pellino-1 protein is an E3 ubiquitin ligase that links ubiquitin to substrate proteins. Pellino-1 Protein, Human (sf9, His-GST) is the recombinant human-derived Pellino-1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of Pellino-1 Protein, Human (sf9, His-GST) is 418 a.a., with molecular weight of ~74.1 kDa.
TRIM24 protein is a multifunctional coactivator that dynamically interacts with nuclear receptors and coactivators to influence target gene transcription. It binds preferentially to unmodified H3K4me0 and acetylated H3K23. TRIM24 Protein, Human (P. pastoris, His) is the recombinant human-derived TRIM24 protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of TRIM24 Protein, Human (P. pastoris, His) is 122 a.a., with molecular weight of 16.5 kDa.
SMURF2 protein is an E3 ubiquitin protein ligase that interacts with SMAD7 to induce ubiquitin-dependent degradation of TGF-β receptors and downregulate TGF-β signaling. This interaction triggers autocatalytic degradation of SMURF2 and is antagonized by AIMP1. SMURF2 Protein, Human (His) is the recombinant human-derived SMURF2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of SMURF2 Protein, Human (His) is 747 a.a., .
SMURF2 protein is an E3 ubiquitin protein ligase that interacts with SMAD7 to induce ubiquitin-dependent degradation of TGF-β receptors and downregulate TGF-β signaling. This interaction triggers autocatalytic degradation of SMURF2 and is antagonized by AIMP1. SMURF2 Protein, Human is the recombinant human-derived SMURF2 protein, expressed by E. coli , with tag free. The total length of SMURF2 Protein, Human is 747 a.a., .
HLTF; Helicase-like transcription factor; DNA-binding protein/plasminogen activator inhibitor 1 regulator; HIP116; RING finger protein 80; RING-type E3 ubiquitin transferase HLTF; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; Sucrose nonfermenting protein 2-like 3
Human
E. coli
HLTF protein has helicase and E3 ubiquitin ligase activities and has intrinsic ATP-dependent nucleosome remodeling ability. It is critical for the transcriptional regulation of specific promoters such as SERPINE1, HIV-1 and SV40. HLTF Protein, Human is the recombinant human-derived HLTF protein, expressed by E. coli , with tag free. The total length of HLTF Protein, Human is 117 a.a., .
