ITCH/AIP4 Protein, Human (solution)

The ITCH/AIP4 protein, a member of the Nedd4 family of ubiquitin ligases, transfers ubiquitin to protein substrates for lysosomal degradation. It regulates cell differentiation and immune responses and is associated with autoimmune disease. Alternative splicing produces multiple isoforms. ITCH/AIP4 is expressed ubiquitously, with notable levels in the testis, esophagus, and 25 other tissues. ITCH/AIP4 Protein, Human (solution) is the recombinant human-derived ITCH/AIP4 protein, expressed by E. coli , with tag free. and with residual Gly-Pro in N-terminal (not related to functional changes) mutation. The total length of ITCH/AIP4 Protein, Human (solution) is 378 a.a., with molecular weight of ~40 kDa.

The ITCH/AIP4 protein, a member of the Nedd4 family of HECT domain E3 ubiquitin ligases, is involved in the transfer of ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, leading to their degradation in lysosomes. This protein has diverse functions, including the regulation of erythroid and lymphoid cell differentiation as well as immune responses. Mutations in this gene have been associated with syndromic multisystem autoimmune disease. Additionally, alternative splicing results in the production of multiple isoforms of this protein. The ITCH/AIP4 protein exhibits ubiquitous expression, with notable levels in tissues such as the testis, esophagus, and 25 other tissues.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

Tag Free

NP_113671.3 (N-G&P, R485-E862)

83737  [NCBI]

Approximately 40 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 200 mM NaCl, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.