ITCH/AIP4 Protein, Human (solution)
Based on 1 Customer Validation
ITCH/AIP4 Protein, Human (solution) is the recombinant human-derived ITCH/AIP4 protein, expressed by E. coli, with tag free tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
ITCH/AIP4 Protein, Human (solution) is the recombinant human-derived ITCH/AIP4 protein, expressed by E. coli, with tag free tag.
The ITCH/AIP4 protein, a member of the Nedd4 family of HECT domain E3 ubiquitin ligases, is involved in the transfer of ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, leading to their degradation in lysosomes. This protein has diverse functions, including the regulation of erythroid and lymphoid cell differentiation as well as immune responses. Mutations in this gene have been associated with syndromic multisystem autoimmune disease. Additionally, alternative splicing results in the production of multiple isoforms of this protein. The ITCH/AIP4 protein exhibits ubiquitous expression, with notable levels in tissues such as the testis, esophagus, and 25 other tissues.
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
NP_113671.3 (N-G&P, R485-E862)
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Molecular Construction
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N-term
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Gly-Pro
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ITCH (R485-E862)
Accession # NP_113671.3 -
C-term
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Protein Length
Partial
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Synonyms
ITCH; Itchy E3 Ubiquitin Protein Ligase Homolog; Itchy E3 Ubiquitin Protein Ligase; HECT-Type E3 Ubiquitin Transferase; AIP4; Atrophin-1 Interacting Protein 4; HECT-Type E3 Ubiquitin Transferase Itchy Homolog; Atrophin-1-Interacting Protein 4; E3 Ubiquiti
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Molecular Weight
Approximately 40 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris, 200 mM NaCl, 10% Glycerol, pH 8.0, 5% trehalose, 5% mannitol and 0.01% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (232 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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