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  4. Sterile Antibiotic Solution
  5. Puromycin, Sterile

Puromycin, Sterile 

Cat. No.: HY-K1057
Manual SDS

MCE Puromycin, Sterile is an aminonucleoside antibiotic produced by Streptomyces alboniger. It inhibits protein synthesis by disrupting peptide transfer on ribosomes, causing premature chain termination during translation. It can kill most gram-positive bacteria and various animal or insect cells.

Puromycin, Sterile
Size Price Stock Quantity
Free Sample (100 μL)   Apply Now  
1 mL USD 71 In-stock
Estimated Time of Arrival: December 31
5 mL USD 254 In-stock
Estimated Time of Arrival: December 31

* Please select Quantity before adding items.

  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

Puromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It inhibits protein synthesis by disrupting peptide transfer on ribosomes, causing premature chain termination during translation. It can kill most gram-positive bacteria and various animal or insect cells.

Resistance to Puromycin is conferred by the puromycin N-acetyl-transferase gene (pac) from Streptomyces alboniger. Therefore, Puromycin is a popular and effective antibiotic for selecting vectors bearing the pac gene in mammalian cell lines and E. coli.

Storage

-20°C; 2 years.

Protocol

1.   The working concentration of Puromycin varies with cell type, media, growth conditions and cell metabolic rate.

2.   Before stable transfected cell lines can be selected, the optimal Puromycin concentration needs to be determined by performing a kill curve titration.

  1)   Seed the parental cell line in a suitable culture plate at a cell density of 20-25% and incubate the cells for 24 hours at 37°C.

  2)   Remove medium and then add medium with various concentrations of Puromycin and incubate at 37°C.

  3)   Refresh the selective medium every 2-3 days and observe the percentage of surviving cells over time.

  4)   Determine the lowest concentration of antibiotic that kills a large majority of the cells within 7-10 days. This concentration should be used for selection of a stable transfected cell line.

Components
Components HY-K1057-1 mL HY-K1057-5 mL
Puromycin, Sterile (10 mg/mL) 1 mL 5 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Puromycin, Sterile
Cat. No.:
HY-K1057
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