Mycoplasma Detection Kit (PCR)

Mycoplasma are small, structurally simple bacteria which lack a cell wall and represent one of the most prevalent and serious sources of cell line contamination. Mycoplasma contamination is invisible, as it causes no discernible change in turbidity or pH even at densities as high as 10⁸ organisms/mL, making it particularly insidious. Mycoplasma infections are highly toxic and can induce a range of changes in cell cultures, including reduced growth rates, abnormal morphology, chromosomal abnormalities, and metabolic disturbances. These changes can lead to slowed cell growth, aberrant differentiation, or even cell death, severely disrupting normal cell functions. Mycoplasma contamination not only significantly affects the reliability, repeatability, and consistency of experimental results but can also lead to experimental failure. Therefore, timely and accurate detection of mycoplasma contamination is crucial for ensuring the quality of cell cultures and the accuracy of experimental outcomes.

MCE Mycoplasma Detection Kit (PCR) is a diagnostic kit that utilizes the PCR method to detect mycoplasma contamination in biological materials such as cultured cells. The kit includes PCR premix and specific primers targeting the conserved regions of the mycoplasma 16S rRNA gene, allowing for the specific amplification of mycoplasma DNA directly using cell culture fluid as a template, thereby enabling the detection of mycoplasma.

Features of MCE Mycoplasma Detection Kit (PCR)

1. High Sensitivity: Capable of detecting as low as 20 copies of mycoplasma.

2. High specificity: PCR amplifies only mycoplasma DNA, without amplifying eukaryotic or bacterial DNA.

3. Easy to Operate: No need for mycoplasma genomic DNA extraction, making it suitable for testing a large number of cell samples.

4. Fast: Detection process can be completed within 2 h.

5. Accurate: Equipped positive and negative controls to ensure the accuracy of PCR results.