Kinase Assay Buffer
Based on 3 publication(s) in Google Scholar
MCE Kinase Assay Buffer can be used for assaying kinase protein. The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
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Storage :
-20°C, 2 years. Protect from light.
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HY-B2176
ATP -
HY-K0013
Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O) -
HY-K0022
Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) -
HY-K0021
Phosphatase Inhibitor Cocktail I (100× in DMSO) -
HY-K0010
Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) -
HY-K0301
Cell Counting Kit-8 -
HY-K0030
Deacetylase Inhibitor Cocktail (100× in 70% DMSO) -
HY-K0012
Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO)
Description & Advantages
MCE Kinase Assay Buffer can be used for assaying kinase protein. This assay buffer is primarily used for Biochemicals applications.
This assay buffer is supplied as a ready-to-use solution in ddH2O. The individual components have specific inhibitory properties. MOPS are commonly used in biology as buffers that maintain the pH of mammalian cell culture media. β-glycerophosphate disodium salt pentahydrate inhibits phosphatase. EGTA is a special calcium chelator. DL-dithiothreitol (DTT) is a strong reducing agent.
Protocol
Thaw at room temperature, add at 1:5 (v/v) dilutions to 1 solution samples and mix thoroughly. It is recommended to prepare solutions of this product fresh when needed.
Publications
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Journal Impact Factor
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Most Recent
Storage
-20°C, 2 years.
Protect from light.
Components
| Components | HY-K0016-1 mL | HY-K0016-10 mL | HY-K0016-50 mL |
|---|---|---|---|
| Kinase Assay Buffer (5×, in ddH2O) | 1 mL | 1 mL × 10 | 1 mL × 50 |
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