Butyl Agarose 4FF  

Hydrophobic Interaction Chromatography (HIC) media are designed to purify biomolecules based on differences in surface hydrophobicity. Common ligands such as butyl or phenyl are covalently attached to agarose or synthetic resins. Proteins bind in high-salt conditions and are gently eluted by decreasing salt concentration, preserving structure and activity. HIC is widely used in downstream processing of peptides, antibodies, and protein therapeutics, from lab-scale to industrial applications.

MCE Butyl Agarose 4FF is a hydrophobic chromatography medium formed by covalently coupling butyl ligands to agarose. It features high mechanical strength, fast flow rate, strong selectivity, high recovery of biological activity, and environmental safety. The medium is resistant to strong acids and bases, allowing for in-place cleaning (CIP) and high-temperature sterilization. Compared to reverse-phase chromatography media, it has a lower ligand density and milder elution conditions, which better preserve the biological activity of target molecules. It is suitable for both laboratory use and large-scale industrial purification.

Each 1 mL of total volume contains 0.5 mL of resin. Thoroughly resuspend the resin before use and mix well before aliquoting.

The specifications of the product correspond to the actual resin volume, with the resin content of 50%.

4°C, 5 years

Do not dry or freeze

1. Ensure the resin is thoroughly resuspended before use.

2. It is recommended to replace the storage buffer every 2-3 months to prevent microbial contamination due to ethanol evaporation.

3. This product is for R&D use only, not for drug, household, or other uses.

4. For your safety and health, please wear a lab coat and disposable gloves to operate.