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  4. Protein Purification
  5. Agarose Beads
  6. Protein G Agarose

Protein G Agarose 

Cat. No.: HY-K0214
Manual COA SDS Technical Support
Magnetic Beads

MCE Protein G Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein G, effectively purifies mammalian monoclonal and polyclonal antibodies, such as human IgG3 and rat IgG2a.

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    Protein G Agarose purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2025 Nov 30:e11683.  [Abstract]

    ITM2B truncation or its point mutants and TSPAN4 were expressed in 786‐O cells, their interactions were detected. Protein G Agarose was used in this study (3 h).

    Protein G Agarose purchased from MCE. Usage Cited in: Int J Biol Macromol. 2025 Sep 9;328(Pt 1):147576.  [Abstract]

    DPV-BAC-CHv50 and DPV-BAC-UL13C3⁎HA were used to infect DEFs, and silver staining was performed 36 h later to detect specific bands. Protein G Agarose (HY-K0214, 2 h) was used in this experiment.

    Protein G Agarose purchased from MCE. Usage Cited in: Int J Biol Macromol. 2025 Oct 16;331(Pt 2):148358.  [Abstract]

    Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) analysis of purified monoclonal antibodies. The results demonstrated that all seven mAbs were purified from mouse ascites via protein G affinity chromatography, yielding highly pure antibodies. In this experiment, Protein G Agarose (HY-K0214) was used for the purification of mouse ascites.

    Protein G Agarose purchased from MCE. Usage Cited in: Int J Biol Macromol. 2025 Sep 9;328(Pt 1):147576.  [Abstract]

    pCAGGS-IFIT5-Flag and pCAGGS-UL13-HA were transfected into duck embryo fibroblasts, and the interaction between IFIT5-Flag and UL13-HA was verified by co-immunoprecipitation. Protein G Agarose (HY-K0214, 2 h) was used in this experiment.

    Protein G Agarose purchased from MCE. Usage Cited in: Int Immunopharmacol. 2025 May 23:159:114913.  [Abstract]

    Immunoprecipitation (IP) was performed using a PKCα or a EP300 antibody to observe the interaction of PKCα and EP300. PKCα and EP300 protein levels in the lysates of HCECs were detected using WB. Protein G Agarose (HY-K0214) was used in this experiment.

    • Description

    • Storage

    • Components

    • Documentation

    Description
    & Advantages

    Protein G is a bacterial cell wall protein isolated from group G Streptococci and binds to most mammalian IgGs mainly through Fc regions. Native Protein G contains 3 IgG binding domains and sites for albumin and cell surface binding. The latter have been eliminated from recombinant Protein G to reduce nonspecific binding.


    MCE Protein G Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein G, effectively purifies mammalian monoclonal and polyclonal antibodies, such as human IgG3 and rat IgG2a.


    Characteristics
    Composition 4% cross-linked agarose
    Degree of Substitution 2 mg Recombinant Protein G/mL Settled Resin
    Bead Diameter 45-165 μm
    Binding Capacity 20 mg Human IgG/mL Settled Resin
    Storage Solution 50% slurry in 1× PBS containing 20% ethanol

     

    The specifications of the product correspond to the actual resin volume, with the resin content of 50%.
    Storage

    Store at 2-8°C, and is stable for at least 2 years.


    Do not dry or freeze.
    Components
    Components HY-K0214-5 mL HY-K0214-10 mL HY-K0214-50 mL
    Protein G Agarose (Settled Resin) 5 mL 10 mL 50 mL
    Documentation

    Manual (307 KB) SDS (251 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Protein G affinity resin

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