Human Bladder Cancer Organoid Kit 

MCE Human Bladder Cancer Organoid Kit contains human bladder cancer organoid basal medium and culture supplement. This product can be used to efficiently construct Human Bladder Cancer organoid.

MCE Human Bladder Cancer Organoid Kit enables the precise reconstruction of the three-dimensional architecture and tumor microenvironment of bladder cancer, providing a stable and reliable platform for in vitro studies of tumor cell proliferation, migration, and drug response. It is well-suited for evaluating the efficacy and toxicity of anticancer drugs, supporting applications in personalized medicine and novel drug screening for bladder cancer.

Basal Medium A: 4°C, 1 year. Shipping with blue ice.

Supplement B (20×) & Supplement C (100×): -20°C, 1 year. Avoid repeated freeze/thaw cycles. Shipping with dry a ice.

1. Tumor cells need to be kept sterile when extracted from primary tumor tissue to avoid contamination from subsequent experiments.

2. Observe the fragmentation status of the organoid during passaged dissociation, and terminate the dissociation when small cell clusters (10-50 cells) appear to avoid prolonging the subsequent growth viability of the organoid.

3. Operations involving MCE Basement Membrane Matrix need to be kept at low temperature throughout. MCE Basement Membrane Matrix should be injected rapidly into the cell culture wells after resuspension with the cells, while avoiding air bubbles.

4. This product is for R&D use only, not for drug, household, or other uses.

5. For your safety and health, please wear a lab coat and disposable gloves to operate.